A Gram-negative, motile, rod-shaped, facultatively anaerobic bacterium was isolated from sediment of Chilika Lagoon, a brackish water lagoon in India. The strain, designated JC5, was able to grow in the presence of 0–8.0 % NaCl and at pH 7.0–10.0. The isolate was positive for oxidase and catalase and exhibited -haemolysis. The major fatty acids were iso-C (18.3 %), C (11.3 %), C 8 (13.1 %) and a summed feature, C 7 and/or C 6 (15.1 %). The genomic DNA G+C content was 54.6 mol%. A phylogenetic tree based on the 16S rRNA gene sequences showed that strain JC5 forms a lineage within the genus and is closely related to DW01 (98.0 %), ATCC 51192 (97.6 %) and C4 (95.8 %). Further, genomic DNA–DNA hybridization of strain JC5 with DW01 and ATCC 51192 showed relatedness of only 42 and 23 %, respectively. On the basis of phenotypic and molecular genetic evidence, strain JC5 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is JC5 (=CCUG 57101 =NBRC 105217 =KCTC 22540).


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vol. , part 12, pp. 3111 - 3115

Biolog GN2 MicroPlate results for sp. nov. JC5 .

Cellular fatty acid compositions of strain JC5 and its closest phylogenetic neighbours.

[PDF file of Supplementary Tables S1 and S2](91 KB)


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