1887

Abstract

A Gram-negative, motile, rod-shaped, facultatively anaerobic bacterium was isolated from sediment of Chilika Lagoon, a brackish water lagoon in India. The strain, designated JC5, was able to grow in the presence of 0–8.0 % NaCl and at pH 7.0–10.0. The isolate was positive for oxidase and catalase and exhibited -haemolysis. The major fatty acids were iso-C (18.3 %), C (11.3 %), C 8 (13.1 %) and a summed feature, C 7 and/or C 6 (15.1 %). The genomic DNA G+C content was 54.6 mol%. A phylogenetic tree based on the 16S rRNA gene sequences showed that strain JC5 forms a lineage within the genus and is closely related to DW01 (98.0 %), ATCC 51192 (97.6 %) and C4 (95.8 %). Further, genomic DNA–DNA hybridization of strain JC5 with DW01 and ATCC 51192 showed relatedness of only 42 and 23 %, respectively. On the basis of phenotypic and molecular genetic evidence, strain JC5 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is JC5 (=CCUG 57101 =NBRC 105217 =KCTC 22540).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.010918-0
2009-12-01
2019-10-17
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/59/12/3111.html?itemId=/content/journal/ijsem/10.1099/ijs.0.010918-0&mimeType=html&fmt=ahah

References

  1. Anzai, Y., Kim, H., Park, J. Y., Wakabayashi, H. & Oyaizu, H. ( 2000; ). Phylogenetic affiliation of the pseudomonads based on 16S rRNA sequence. Int J Syst Evol Microbiol 50, 1563–1589.[CrossRef]
    [Google Scholar]
  2. Benson, H. J. ( 2002; ). Microbiological Applications: A Laboratory Manual in General Microbiology, 8th edn. New York: McGraw-Hill.
  3. Biebl, H. & Pfennig, N. ( 1981; ). Isolation of members of the family Rhodospirillaceae. In The Prokaryotes, vol. 1, pp. 267–273. Edited by M. P. Starr, H. Stolp, H. G. Trüper, A. Balows & H. G. Schlegel. Berlin: Springer.
  4. Bowman, J. P. ( 2005; ). Genus XIII. Shewanella. In Bergey's Manual of Systematic Bacteriology, 2nd edn, vol. 2, part B, pp. 480–491. Edited by D. J. Brenner, N. R. Krieg, J. T. Staley & G. M. Garrity. New York: Springer.
  5. Cappuccino, J. G. & Sherman, N. ( 1999; ). Microbiology: a Laboratory Manual, 5th edn. Menlo Park, CA: Benjamin/Cummings.
  6. Chun, J., Bae, K. S., Moon, E. Y., Jung, S. O., Lee, H. K. & Kim, S. J. ( 2000; ). Nocardiopsis kunsanensis sp. nov., a moderately halophilic actinomycete isolated from a saltern. Int J Syst Evol Microbiol 50, 1909–1913.
    [Google Scholar]
  7. CLSI ( 2003; ). Performance standards for antimicrobial disk susceptibility tests, 8th edn. Approved Standard M2-A8. Wayne, PA: Clinical Laboratory Standards Institute.
  8. Felsenstein, J. ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  9. Fitch, W. M. & Margoliash, E. ( 1967; ). Construction of phylogenetic trees. Science 155, 279–284.[CrossRef]
    [Google Scholar]
  10. Gauthier, G., Gauthier, M. & Christen, R. ( 1995; ). Phylogenetic analysis of the genera Alteromonas, Shewanella, and Moritella using genes coding for small-subunit rRNA sequences and division of the genus Alteromonas into two genera, Alteromonas (emended) and Pseudoalteromonas gen. nov., and proposal of twelve new species combinations. Int J Syst Bacteriol 45, 755–761.[CrossRef]
    [Google Scholar]
  11. Guindon, S. & Gascuel, O. ( 2003; ). A simple fast and accurate algorithm to estimate large phylogenies by maximum likelihood. Syst Biol 52, 696–704.[CrossRef]
    [Google Scholar]
  12. Imhoff, J. F., Süling, J. & Petri, R. ( 1998; ). Phylogenetic relationships among the Chromatiaceae, their taxonomic reclassification and description of the new genera Allochromatium, Halochromatium, Isochromatium, Marichromatium, Thiococcus, Thiohalocapsa and Thermochromatium. Int J Syst Bacteriol 48, 1129–1143.[CrossRef]
    [Google Scholar]
  13. Jukes, T. H. & Cantor, C. R. ( 1969; ). Evolution of protein molecules. In Mammalian Protein Metabolism, vol. 3, pp. 21–132. Edited by H. N. Munro. New York: Academic Press.
  14. Kim, D., Baik, K. S., Kim, M. S., Jung, B. M., Shin, T. S., Chung, G. H., Rhee, M. S. & Seong, C. N. ( 2007; ). Shewanella haliotis sp. nov., isolated from the gut microflora of abalone, Haliotis discus hannai. Int J Syst Evol Microbiol 57, 2926–2931.[CrossRef]
    [Google Scholar]
  15. MacDonell, M. T. & Colwell, R. R. ( 1985; ). Phylogeny of the Vibrionaceae, and recommendation for two new genera, Listonella and Shewanella. Syst Appl Microbiol 6, 171–182.[CrossRef]
    [Google Scholar]
  16. Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  17. Nozue, H., Hayashi, T., Hashimoto, Y., Ezaki, T., Hamasaki, K., Ohwada, K. & Terawaki, Y. ( 1992; ). Isolation and characterization of Shewanella alga from human clinical specimens and emendation of the description of S. alga Simidu et al., 1990, 335. Int J Syst Bacteriol 42, 628–634.[CrossRef]
    [Google Scholar]
  18. Park, S. C., Baik, K. S., Kim, M. S., Kim, D. & Seong, C. N. ( 2009; ). Shewanella marina sp. nov., isolated from seawater. Int J Syst Evol Microbiol 59, 1888–1894.[CrossRef]
    [Google Scholar]
  19. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  20. Sasser, M. ( 1990; ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
  21. Seldin, L. & Dubnau, D. ( 1985; ). Deoxyribonucleic acid homology among Bacillus polymyxa, Bacillus macerans, Bacillus azotofixans, and other nitrogen-fixing Bacillus strains. Int J Syst Bacteriol 35, 151–154.[CrossRef]
    [Google Scholar]
  22. Simidu, U., Kita-Tsukamoto, K., Yasumoto, T. & Yotsu, M. ( 1990; ). Taxonomy of four marine bacterial strains that produce tetrodotoxin. Int J Syst Bacteriol 40, 331–336.[CrossRef]
    [Google Scholar]
  23. Swofford, D. L. ( 1998; ). Phylogenetic Analysis Using Parsimony (paup). Version 4. Sunderland, MA: Sinauer Associates.
  24. Venkateswaran, K., Moser, D. P., Dollhopf, M. E., Lies, D. P., Saffarini, D. A., MacGregor, B. J., Ringelberg, D. B., White, D. C., Nishijima, M. & other authors ( 1999; ). Polyphasic taxonomy of the genus Shewanella and description of Shewanella oneidensis sp. nov. Int J Syst Bacteriol 49, 705–724.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.010918-0
Loading
/content/journal/ijsem/10.1099/ijs.0.010918-0
Loading

Data & Media loading...

Supplements

vol. , part 12, pp. 3111 - 3115

Biolog GN2 MicroPlate results for sp. nov. JC5 .

Cellular fatty acid compositions of strain JC5 and its closest phylogenetic neighbours.

[PDF file of Supplementary Tables S1 and S2](91 KB)



PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error