1887

Abstract

A pink-red pigmented, non-motile, coccoid bacterial strain, ST0316, was isolated from dust samples collected from the stratosphere in Japan. Phylogenetic analysis based on 16S rRNA gene sequences showed that it belonged to the genus . DNA G+C content (69.8 mol%), desiccation tolerance, and resistance to gamma-rays [D (dose required to reduce the bacterial population by 10-fold) >8 kGy] and UV radiation (D 1000 J m) supported the affiliation of strain ST0316 to the genus . The major peptidoglycan amino acids were -glutamic acid, glycine, -alanine, -alanine and ornithine. Predominant fatty acids were C 7, C, C and iso-C. Strain ST0316 diverged from recognized species of the genus , showing less than 93.0 % similarity values to its closest relatives , , and . Strain ST0316 also differed from the type strains of closely related species in its polar lipid profile, nitrate reduction and carbon-source assimilation tests. Therefore, we propose a new species of the genus , sp. nov. (type strain, ST0316 =JCM 11751 =DSM 21230).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.010876-0
2010-04-01
2021-04-15
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/60/4/776.html?itemId=/content/journal/ijsem/10.1099/ijs.0.010876-0&mimeType=html&fmt=ahah

References

  1. Battista J. R., Rainey F. A. 2001; Family I. Deinococcaceae Brooks and Murray 1981, 356,VP emend. Rainey, Nobre, Schumann, Stackebrandt and da Costa 1997, 513. In Bergey's Manual of Systematic Bacteriology , 2nd edn. vol 1 pp 395–396 Edited by Boone D. R., Castenholz R. W., Garrity G. M. New York: Springer;
    [Google Scholar]
  2. Callegan R. P., Nobre M. F., McTernan P. M., Battista J. R., Navarro-González R., McKay C. P., da Costa M. S., Rainey F. A. 2008; Description of four novel psychrophilic, ionizing radiation-sensitive Deinococcus species from alpine environments. Int J Syst Evol Microbiol 58:1252–1258 [CrossRef]
    [Google Scholar]
  3. Castresana J. 2000; Selection of conserved blocks from multiple alignments for their use in phylogenetic analysis. Mol Biol Evol 17:540–552 [CrossRef]
    [Google Scholar]
  4. Christensen E. A., Kristensen H. 1981; Radiation-resistance of micro-organisms from air in clean premises. Acta Pathol Microbiol Scand [B] 89:293–301
    [Google Scholar]
  5. Degryse E., Glansdorff N., Pierard A. 1978; A comparative analysis of extreme thermophilic bacteria belonging to the genus Thermus . Arch Microbiol 117:189–196 [CrossRef]
    [Google Scholar]
  6. Embley T. M., O’Donnell A. G., Watt R., Rostron J. 1987; Lipid and cell wall amino acid composition in the classification of members of the genus Deinococcus . Syst Appl Microbiol 10:20–27 [CrossRef]
    [Google Scholar]
  7. Ferreira A. C., Nobre M. F., Rainey F. A., Silva M. T., Wait R., Burghardt J., Chung A. P., da Costa M. S. 1997; Deinococcus geothermalis sp. nov. and Deinococcus murrayi sp. nov., two extremely radiation-resistant and slightly thermophilic species from hot springs. Int J Syst Bacteriol 47:939–947 [CrossRef]
    [Google Scholar]
  8. Hiraishi A., Masamune K., Kitamura H. 1989; Characterization of the bacterial population structure in an anaerobic-aerobic activated sludge system on the basis of respiratory quinone profiles. Appl Environ Microbiol 55:897–901
    [Google Scholar]
  9. Hirsch P., Gallikowski C. A., Siebert J., Peissl K., Kroppenstedt R., Schumann P., Stackebrandt E., Anderson R. 2004; Deinococcus frigens sp. nov., Deinococcus saxicola sp. nov., and Deinococcus marmoris sp. nov., low temperature and draught-tolerating, UV-resistant bacteria from continental Antarctica. Syst Appl Microbiol 27:636–645 [CrossRef]
    [Google Scholar]
  10. Jobb G. 2008; treefinder, version of; June 2008; Distributed by the author. Munich, Germany: http://www.treefinder.de
  11. Kumar S., Tamura K., Nei M. 2004; mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5:150–163 [CrossRef]
    [Google Scholar]
  12. Kuykendall L. D., Roy M. A., O’Neill J. J., Devine T. E. 1988; Fatty acids, antibiotic resistance, and deoxyribonucleic acid homology groups of Bradyrhizobium japonicum . Int J Syst Bacteriol 38:358–361 [CrossRef]
    [Google Scholar]
  13. Lai W. A., Kämpfer P., Arun A. B., Shen F. T., Huber B., Rekha P. D., Young C. C. 2006; Deinococcus ficus sp. nov., isolated from the rhizosphere of Ficus religiosa L. Int J Syst Evol Microbiol 56:787–791 [CrossRef]
    [Google Scholar]
  14. Murray R. G. E., Robinow C. F. 1958; Cytological studies of a tetrad-forming coccus. In Proceedings of the 7th International Congress of Microbiologists, Stockholm pp 427–428
    [Google Scholar]
  15. Posada D., Crandall K. A. 1998; Modeltest: testing the model of DNA substitution. Bioinformatics 14:817–818 [CrossRef]
    [Google Scholar]
  16. Rainey F. A., Ray K., Ferreira M., Gatz B. Z., Nobre M. F., Bagaley D., Rash B. A., Park M. J., Earl A. M. other authors 2005; Extensive diversity of ionizing-radiation-resistant bacteria recovered from Sonoran Desert soil and description of nine new species of the genus Deinococcus obtained from a single soil sample. Appl Environ Microbiol 71:5225–5235 [CrossRef]
    [Google Scholar]
  17. Tatusova T. A., Madden T. L. 1999; blast 2 Sequences, a new tool for comparing protein and nucleotide sequences. FEMS Microbiol Lett 174:247–250 [CrossRef]
    [Google Scholar]
  18. Thompson J. D., Higgins D. G., Gibson T. J. 1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680 [CrossRef]
    [Google Scholar]
  19. Tindall B. J. 1990; Lipid composition of Halobacterium lacusprofundi . FEMS Microbiol Lett 66:199–202 [CrossRef]
    [Google Scholar]
  20. Weon H. Y., Kim B. Y., Schumann P., Son J. A., Jang J., Go S. J., Kwon S. W. 2007; Deinococcus cellulosilyticus sp. nov., isolated from air. Int J Syst Evol Microbiol 57:1685–1688 [CrossRef]
    [Google Scholar]
  21. Yang Y., Itahashi S., Yokobori S., Yamagishi A. 2008; UV-resistant bacteria isolated from upper troposphere and lower stratosphere. Biol Sci Space 22:18–25 [CrossRef]
    [Google Scholar]
  22. Yang Y., Itoh T., Yokobori S., Itahashi S., Shimada H., Satoh K., Ohba H., Narumi I., Yamagishi A. 2009; Deinococcus aerius sp. nov., isolated from the high atmosphere. Int J Syst Evol Microbiol 59:1862–1866 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.010876-0
Loading
/content/journal/ijsem/10.1099/ijs.0.010876-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error