RT Journal Article SR Electronic(1) A1 Zhang, Jia-Yue A1 Liu, Xing-Yu A1 Liu, Shuang-JiangYR 2009 T1 Nocardioides terrae sp. nov., isolated from forest soil JF International Journal of Systematic and Evolutionary Microbiology, VO 59 IS 10 SP 2444 OP 2448 DO https://doi.org/10.1099/ijs.0.010785-0 PB Microbiology Society, SN 1466-5034, AB Bacterial strain VA15T was isolated from forest soil of the Changbai Mountains, Heilongjiang Province, China. Cells of strain VA15T were Gram-positive-staining, 0.2–0.3 μm in diameter and 0.3–1.0 μm long, strictly aerobic, non-motile and rod-shaped or coccoid. Growth occurred at 16–34 °C (optimum, 29 °C), at pH 5.5–8.5 (optimum, pH 6.2–6.5) and in the presence of 0–1 % (w/v) NaCl (optimum, 0–0.25 %). Strain VA15T contained MK-8(H4) as the major respiratory quinone and ll-2,6-diaminopimelic acid as the cell-wall diamino acid. The major cellular fatty acids were iso-C16 : 0 (18.1 % of the total), 10-methyl C18 : 0 (11.8 %) and 10-methyl C17 : 0 (10.7 %). The DNA G+C content of strain VA15T was 71.6 mol% (T m). 16S rRNA gene sequence analysis indicated that strain VA15T was phylogenetically related to members of the genus Nocardioides; levels of similarity to the type strains of recognized Nocardioides species ranged from 92.9 to 97.7 %. Levels of DNA–DNA relatedness between strain VA15T and its closest phylogenetic relatives, Nocardioides panacihumi DSM 18660T and Nocardioides marinus DSM 18248T, were 21 and 16 %, respectively. Based on these results, it is concluded that strain VA15T represents a novel species of the genus Nocardioides, for which the name Nocardioides terrae sp. nov. is proposed. The type strain is VA15T (=CGMCC 1.7056T =NBRC 104259T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.010785-0