1887

Abstract

Bacterial strain VA15 was isolated from forest soil of the Changbai Mountains, Heilongjiang Province, China. Cells of strain VA15 were Gram-positive-staining, 0.2–0.3 μm in diameter and 0.3–1.0 μm long, strictly aerobic, non-motile and rod-shaped or coccoid. Growth occurred at 16–34 °C (optimum, 29 °C), at pH 5.5–8.5 (optimum, pH 6.2–6.5) and in the presence of 0–1 % (w/v) NaCl (optimum, 0–0.25 %). Strain VA15 contained MK-8(H) as the major respiratory quinone and -2,6-diaminopimelic acid as the cell-wall diamino acid. The major cellular fatty acids were iso-C (18.1 % of the total), 10-methyl C (11.8 %) and 10-methyl C (10.7 %). The DNA G+C content of strain VA15 was 71.6 mol% ( ). 16S rRNA gene sequence analysis indicated that strain VA15 was phylogenetically related to members of the genus ; levels of similarity to the type strains of recognized species ranged from 92.9 to 97.7 %. Levels of DNA–DNA relatedness between strain VA15 and its closest phylogenetic relatives, DSM 18660 and DSM 18248, were 21 and 16 %, respectively. Based on these results, it is concluded that strain VA15 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is VA15 (=CGMCC 1.7056 =NBRC 104259).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.010785-0
2009-10-01
2021-07-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/59/10/2444.html?itemId=/content/journal/ijsem/10.1099/ijs.0.010785-0&mimeType=html&fmt=ahah

References

  1. Altschul, S. F., Gish, W., Miller, W., Myers, E. W. & Lipman, D. J.(1990). Basic local alignment search tool. J Mol Biol 215, 403–410.[CrossRef] [Google Scholar]
  2. An, D. S., Im, W. T., Lee, S. T. & Yoon, M. H.(2007).Nocardioides panacihumi sp. nov., isolated from soil of a ginseng field. Int J Syst Evol Microbiol 57, 2143–2146.[CrossRef] [Google Scholar]
  3. Choi, D. H., Kim, H. M., Noh, J. H. & Cho, B. C.(2007).Nocardioides marinus sp. nov. Int J Syst Evol Microbiol 57, 775–779.[CrossRef] [Google Scholar]
  4. Collins, M. D.(1985). Isoprenoid quinone analysis in classification and identification. In Chemical Methods in Bacterial Systematics, pp. 267–287. Edited by M. Goodfellow & D. E. Minnikin. London: Academic Press.
  5. Davis, K. E., Joseph, S. J. & Janssen, P. H.(2005). Effects of growth medium, inoculum size, and incubation time on culturability and isolation of soil bacteria. Appl Environ Microbiol 71, 826–834.[CrossRef] [Google Scholar]
  6. De Ley, J., Cattoir, H. & Reynaerts, A.(1970). The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12, 133–142.[CrossRef] [Google Scholar]
  7. Dong, X.-Z. & Cai, M.-Y.(2001).Determinative Manual for Routine Bacteriology. Beijing: Scientific Press (English translation).
  8. Gerhardt, P., Murray, R. G. E., Wood, W. A. & Krieg, N. R. (editors)(1994).Methods for General and Molecular Bacteriology. Washington, DC: American Society for Microbiology.
  9. Hu, Y.-T., Zhou, P.-J., Zhou, Y.-G., Liu, Z.-H. & Liu, S.-J.(2004).Saccharothrix xingjiangensis sp. nov., a pyrene-degrading actinomycete isolated from Tianchi Lake, Xinjiang, China. Int J Syst Evol Microbiol 54, 2091–2094.[CrossRef] [Google Scholar]
  10. Huß, V. A. R., Festl, H. & Schleifer, K. H.(1983). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4, 184–192.[CrossRef] [Google Scholar]
  11. Kumar, S., Tamura, K. & Nei, M.(2004).mega 3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef] [Google Scholar]
  12. Lee, S. D.(2007).Nocardioides furvisabuli sp. nov., isolated from black sand. Int J Syst Evol Microbiol 57, 35–39.[CrossRef] [Google Scholar]
  13. Lee, D. W., Hyun, C.-G. & Lee, S. D.(2007).Nocardioides marinisabuli sp. nov., a novel actinobacterium isolated from beach sand. Int J Syst Evol Microbiol 57, 2960–2963.[CrossRef] [Google Scholar]
  14. Li, B., Xie, C.-H. & Yokota, A.(2007).Nocardioides exalbidus sp. nov., a novel actinomycete isolated from lichen in Izu-Oshima Island, Japan. Actinomycetologica 21, 22–26.[CrossRef] [Google Scholar]
  15. Marmur, J. & Doty, P.(1962). Determination of the base composition of deoxyribonucleic acid from thermal denaturation temperature. J Mol Biol 5, 109–118.[CrossRef] [Google Scholar]
  16. Prauser, H.(1976).Nocardioides, a new genus of the order Actinomycetales. Int J Syst Bacteriol 26, 58–65.[CrossRef] [Google Scholar]
  17. Reasoner, D. J. & Geldreich, E. E.(1985). A new medium for the enumeration and subculture of bacteria from potable water. Appl Environ Microbiol 49, 1–7. [Google Scholar]
  18. Sait, M., Hugenholtz, P. & Janssen, P. H.(2002). Cultivation of globally distributed soil bacteria from phylogenetic lineages previously only detected in cultivation-independent surveys. Environ Microbiol 4, 654–666.[CrossRef] [Google Scholar]
  19. Staneck, J. L. & Roberts, G. D.(1974). Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 28, 226–231. [Google Scholar]
  20. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G.(1997). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef] [Google Scholar]
  21. Tóth, E. M., Kéki, Z., Homonnay, Z. G., Borsodi, A. K., Márialigeti, K. & Schumann, P.(2008).Nocardioides daphniae sp. nov., isolated from Daphnia cucullata (Crustacea: Cladocera). Int J Syst Evol Microbiol 58, 78–83.[CrossRef] [Google Scholar]
  22. Wu, C., Lu, X., Qin, M., Wang, Y. & Ruan, J.(1989). Analysis of menaquinone compound in microbial cells by HPLC. Microbiology [English translation of Microbiology (Beijing)] 16, 176–178. [Google Scholar]
  23. Yi, H. & Chun, J.(2004a).Nocardioides ganghwensis sp. nov., isolated from tidal flat sediment. Int J Syst Evol Microbiol 54, 1295–1299.[CrossRef] [Google Scholar]
  24. Yi, H. & Chun, J.(2004b).Nocardioides aestuarii sp. nov., isolated from tidal flat sediment. Int J Syst Evol Microbiol 54, 2151–2154.[CrossRef] [Google Scholar]
  25. Yoon, J.-H., Rhee, S.-K., Lee, J.-S., Park, Y.-H. & Lee, S. T.(1997).Nocardioides pyridinolyticus sp. nov., a pyridine-degrading bacterium isolated from the oxic zone of an oil shale column. Int J Syst Bacteriol 47, 933–938.[CrossRef] [Google Scholar]
  26. Yoon, J.-H., Kim, I.-G., Kang, K. H., Oh, T.-K. & Park, Y.-H.(2004).Nocardioides aquiterrae sp. nov., isolated from groundwater in Korea. Int J Syst Evol Microbiol 54, 71–75.[CrossRef] [Google Scholar]
  27. Yoon, J.-H., Kim, I.-G., Lee, M.-H. & Oh, T.-K.(2005).Nocardioides kribbensis sp. nov., isolated from an alkaline soil. Int J Syst Evol Microbiol 55, 1611–1614.[CrossRef] [Google Scholar]
  28. Yoon, J.-H., Lee, C.-H. & Oh, T.-K.(2006a).Nocardioides lentus sp. nov., isolated from an alkaline soil. Int J Syst Evol Microbiol 56, 271–275.[CrossRef] [Google Scholar]
  29. Yoon, J.-H., Lee, J.-K., Jung, S.-Y., Kim, J.-A., Kim, H.-K. & Oh, T.-K.(2006b).Nocardioides kongjuensis sp. nov., an N-acylhomoserine lactone-degrading bacterium. Int J Syst Evol Microbiol 56, 1783–1787.[CrossRef] [Google Scholar]
  30. Yoon, J.-H., Kang, S.-J., Lee, C.-H. & Oh, T.-K.(2007).Nocardioides insulae sp. nov., isolated from soil. Int J Syst Evol Microbiol 57, 136–140.[CrossRef] [Google Scholar]
  31. Zhang, D., Yang, H., Zhang, W., Huang, Z. & Liu, S.-J.(2003).Rhodocista pekingensis sp. nov., a cyst-forming phototrophic bacterium from a municipal wastewater treatment plant. Int J Syst Evol Microbiol 53, 1111–1114.[CrossRef] [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.010785-0
Loading
/content/journal/ijsem/10.1099/ijs.0.010785-0
Loading

Data & Media loading...

Supplements

[PDF file of Supplementary Figs S1 and S2](65 KB)

PDF

Scanning electron micrograph of cells of sp. nov. VA15 grown on R2A agar at 30 °C for 3 days. Bar, 5.0 µm.

IMAGE

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error