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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 59, Issue 10

sp. nov., isolated from forest soil Free

Abstract

Bacterial strain VA15 was isolated from forest soil of the Changbai Mountains, Heilongjiang Province, China. Cells of strain VA15 were Gram-positive-staining, 0.2–0.3 μm in diameter and 0.3–1.0 μm long, strictly aerobic, non-motile and rod-shaped or coccoid. Growth occurred at 16–34 °C (optimum, 29 °C), at pH 5.5–8.5 (optimum, pH 6.2–6.5) and in the presence of 0–1 % (w/v) NaCl (optimum, 0–0.25 %). Strain VA15 contained MK-8(H) as the major respiratory quinone and -2,6-diaminopimelic acid as the cell-wall diamino acid. The major cellular fatty acids were iso-C (18.1 % of the total), 10-methyl C (11.8 %) and 10-methyl C (10.7 %). The DNA G+C content of strain VA15 was 71.6 mol% ( ). 16S rRNA gene sequence analysis indicated that strain VA15 was phylogenetically related to members of the genus ; levels of similarity to the type strains of recognized species ranged from 92.9 to 97.7 %. Levels of DNA–DNA relatedness between strain VA15 and its closest phylogenetic relatives, DSM 18660 and DSM 18248, were 21 and 16 %, respectively. Based on these results, it is concluded that strain VA15 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is VA15 (=CGMCC 1.7056 =NBRC 104259).

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2009-10-01
2025-11-14

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Nocardioides terrae sp. nov., isolated from forest soil
Int J Syst Evol Microbiol 59, 2444 (2009); https://doi.org/10.1099/ijs.0.010785-0
/content/journal/ijsem/10.1099/ijs.0.010785-0
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[PDF file of Supplementary Figs S1 and S2](65 KB)

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Scanning electron micrograph of cells of sp. nov. VA15 grown on R2A agar at 30 °C for 3 days. Bar, 5.0 µm.

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