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Abstract

A Gram-stain-negative, rod-shaped bacterium, designated strain YC7378 was isolated from vermicompost (VC) collected at Masan, Korea, and its taxonomic position was investigated by using a polyphasic approach. Strain YC7378 grew optimally at 30 °C and at pH 6.5–8.5. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain YC7378 belongs to the genus S in the family . The most closely related strains are KSL-125 (95.7 %), 3-2W4 (95.6 %) and Y2 (95.5 %). Strain YC7378 contained ubiquinone Q-10 as the major respiratory quinone system and -homospermidine as the major polyamine. The major fatty acids of strain YC7378 were C 7, C 7 and/or iso-C 2-OH, C 2-OH and C. The major polar lipids were sphingoglycolipid, diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The total DNA G+C content was 59.4 mol%. The phenotypic, phylogenetic and chemotaxonomic data showed that strain YC7378 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is YC7378 (=KCTC 22446 =DSM 21593).

Keyword(s): VC, vermicompost
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2010-03-01
2019-10-14
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vol. , part 3, pp. 580 - 584

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Transmission electron micrograph of strain YC7378 , 24-h-old culture cultivated in half-strength R2A broth. Bar, 200 nm.

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Phylogenetic tree reconstructed from the comparative analysis of 16S rRNA gene sequences showing the relationship of strain YC7378 , with other related taxa. The phylogenetic tree was reconstructed by using the maximum-likelihood method and data obtained from aligned nucleotides. Bar, 1 substitution per 10 nucleotide positions. [ PDF] (15 KB)

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