1887

Abstract

Two bacterial strains, KMM 3891 and KMM 3892, were isolated from internal tissues of the marine mollusc collected from the Sea of Japan. The novel isolates were Gram-negative, aerobic, faint pink–reddish-pigmented, rod-shaped, non-motile, stenohaline and psychrotolerant bacteria that were unable to degrade most tested complex polysaccharides. Polar lipids consisted of phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. Fatty acid analysis revealed C 6, C, C and C 7 as the dominant components. The major isoprenoid quinone was Q-7. The DNA G+C content of strain KMM 3891 was 51.7 mol%. According to phylogenetic analysis of 16S rRNA gene sequences, strains KMM 3891 and KMM 3892 were positioned within the as a separate branch, sharing <93 % sequence similarity to their phylogenetic relatives including , species, , and . Based on phenotypic characterization and phylogenetic distance, the novel marine isolates KMM 3891 and KMM 3892 represent a new genus and species, for which the name gen. nov., sp. nov. is proposed. The type strain of is KMM 3891 (=NRIC 0753 =JCM 15738).

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2010-03-01
2019-12-11
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References

  1. Baumann, P. & Baumann, L. ( 1981; ).The marine Gram-negative eubacteria: genera Photobacterium, Beneckea, Alteromonas, Pseudomonas and Alcaligenes. In The Prokaryotes, vol. 1, pp. 1302–1330. Edited byM. P. Starr, H. Stolp, H. G. Trüper, A. Balows & H. Schlegel. Berlin:Springer.
  2. Blackall, L. L., Hayward, A. C. & Sly, L. I. ( 1985; ). Cellulolytic and dextranolytic gram-negative bacteria: revivalof the genus Cellvibrio. J Appl Bacteriol 59, 81–97.[CrossRef]
    [Google Scholar]
  3. Bligh, E. G. & Dyer, W. J. ( 1959; ).A rapid method of total lipid extraction and purification. CanJ Biochem Physiol 37, 911–917.
    [Google Scholar]
  4. Distel, D. L., Morrill, W., MacLaren-Toussaint, N., Franks,D. & Waterbury, J. ( 2002; ). Teredinibacterturnerae gen. nov., sp. nov., a dinitrogen-fixing, cellulolytic, endosymbiotic γ-proteobacterium isolated from the gills of wood-boring molluscs (Bivalvia:Teredinidae). Int J Syst Evol Microbiol 52, 2261–2269.[CrossRef]
    [Google Scholar]
  5. Ekborg, N. A., Gonzalez, J. M., Howard, M. B., Taylor, L. E.,Hutcheson, S. W. & Weiner, R. M. ( 2005; ). Saccharophagus degradans gen. nov., sp. nov., versatile marine degraderof complex polysaccharides. Int J Syst Evol Microbiol 55, 1545–1549.[CrossRef]
    [Google Scholar]
  6. Ezaki, T., Hashimoto, Y. & Yabuuchi, E. ( 1989; ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridizationin microdilution wells as an alternative to membrane filter hybridizationin which radioisotopes are used to determine genetic relatedness among bacterialstrains. Int J Syst Bacteriol 39, 224–229.[CrossRef]
    [Google Scholar]
  7. Gerhardt, P., Murray, R. G. E., Wood, W. A. & Krieg, N.R. (editors) ( 1994; ). Methods for Generaland Molecular Bacteriology. Washington, DC: American Society for Microbiology.
  8. Gonzalez, J. M. & Weiner, R. M. ( 2000; ). Phylogenetic characterization of marine bacterium strain 2-40, a degraderof complex polysaccharides. Int J Syst Evol Microbiol 50, 831–834.[CrossRef]
    [Google Scholar]
  9. Gonzalez, J. M., Mayer, F., Moran, A., Hodson, R. E. & Whitman,W. ( 1997; ). Microbulbifer hydrolyticus gen.nov., sp. nov., and Marinobacterium georgiense gen. nov., sp. nov.,two marine bacteria from a lignin-rich pulp mill waste enrichment community. Int J Syst Bacteriol 47, 369–376.[CrossRef]
    [Google Scholar]
  10. Humphry, D. R., Black, G. W. & Cummings, S. P. ( 2003; ). Reclassification of ‘Pseudomonas fluorescenssubsp. cellulosa’ NCIMB 10462 (Ueda et al. 1952)as Cellvibrio japonicus sp. nov. and revival of Cellvibrio vulgaris sp. nov., nom. rev. and Cellvibrio fulvus sp. nov., nom. rev. Int J Syst Evol Microbiol 53, 393–400.[CrossRef]
    [Google Scholar]
  11. Kurahashi, M. & Yokota, A. ( 2007; ). Endozoicomonas elysicola gen. nov., sp. nov., a γ-proteobacteriumisolated from the sea slug Elysia ornata. Syst Appl Microbiol 30, 202–206.[CrossRef]
    [Google Scholar]
  12. Leifson, E. ( 1963; ). Determination ofcarbohydrate metabolism of marine bacteria. J Bacteriol 85, 1183–1184.
    [Google Scholar]
  13. Marmur, J. & Doty, P. ( 1962; ). Determinationof the base composition of deoxyribonucleic acid from its thermal denaturationtemperature. J Mol Biol 5, 109–118.[CrossRef]
    [Google Scholar]
  14. Mergaert, J., Lednicka, D., Goris, J., Cnockaert, M. C., DeVos, P. & Swings, J. ( 2003; ). Taxonomic study of Cellvibrio strains and description of Cellvibrio ostraviensissp. nov., Cellvibrio fibrivorans sp. nov. and Cellvibrio gandavensis sp. nov. Int J Syst Evol Microbiol 53, 465–471.[CrossRef]
    [Google Scholar]
  15. Miyazaki, M., Nogi, Y., Ohta, Y., Hatada, Y., Fujiwara, Y.,Ito, S. & Horikoshi, K. ( 2008; ). Microbulbiferagarilyticus sp. nov. and Microbulbifer thermotolerans sp. nov.,agar-degrading bacteria isolated from deep-sea sediment. Int JSyst Evol Microbiol 58, 1128–1133.
    [Google Scholar]
  16. Owen, R. J., Hill, L. R. & Lapage, S. P. ( 1969; ). Determination of DNA base composition from melting profilesin dilute buffers. Biopolymers 7, 503–516.[CrossRef]
    [Google Scholar]
  17. Pearson, W. R. & Lipman, D. J. ( 1988; ). Improved tools for biological sequence comparison. Proc NatlAcad Sci U S A 85, 2444–2448.
    [Google Scholar]
  18. Romanenko, L. A., Schumann, P., Zhukova, N. V., Rohde, M., Mikhailov,V. V. & Stackebrandt, E. ( 2003; ). Oceanisphaeralitoralis gen. nov., sp. nov., a novel halophilic bacterium from marinebottom sediments. Int J Syst Evol Microbiol 53, 1885–1888.[CrossRef]
    [Google Scholar]
  19. Romanenko, L. A., Schumann, P., Rohde, M., Mikhailov, V. V. &Stackebrandt, E. ( 2004; ). Reinekea marinisedimentorum gen. nov., sp. nov., a novel gammaproteobacterium from marine coastalsediments. Int J Syst Evol Microbiol 54, 669–673.[CrossRef]
    [Google Scholar]
  20. Romanenko, L. A., Uchino, M., Falsen, E., Frolova, G. M., Zhukova,N. V. & Mikhailov, V. V. ( 2005; ). Pseudomonaspachastrellae sp. nov., isolated from a marine sponge. IntJ Syst Evol Microbiol 55, 919–924.
    [Google Scholar]
  21. Romanenko, L. A., Uchino, M., Frolova, G. M., Tanaka, N., Kalinovskaya,N. I., Latyshev, N. & Mikhailov, V. V. ( 2007; ). Sphingomonas molluscorum sp. nov., a novel marine isolate with antimicrobialactivity. Int J Syst Evol Microbiol 57, 358–363.[CrossRef]
    [Google Scholar]
  22. Shida, O., Takagi, H., Kadowaki, K., Nakamura, L. K. & Komagata,K. ( 1997; ). Transfer of Bacillus alginolyticus, Bacillus chondroitinus, Bacillus curdlanolyticus, Bacillus glucanolyticus, Bacillus kobensis, and Bacillusthiaminolyticus to the genus Paenibacillus and emended descriptionof the genus Paenibacillus. Int J Syst Bacteriol 47, 289–298.[CrossRef]
    [Google Scholar]
  23. Shieh, W. Y., Liu, T. Y., Lin, S. Y., Jean, W. D. & Chen,J. S. ( 2008; ). Simiduia agarivorans gen. nov.,sp. nov., a marine, agarolytic bacterium isolated from shallow coastal waterfrom Keelung, Taiwan. Int J Syst Evol Microbiol 58, 895–900.[CrossRef]
    [Google Scholar]
  24. Smibert, R. M. & Krieg, N. R. ( 1994; ). Phenotypic characterization. In Methods for General and MolecularBacteriology, pp. 607–654. Edited by P. Gerhardt, R. G. E. Murray,W. A. Wood & N. R. Krieg. Washington, DC: American Society for Microbiology.
  25. Tamura, K., Dudley, J., Nei, M. & Kumar, S. ( 2007; ). mega 4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24, 1596–1599.[CrossRef]
    [Google Scholar]
  26. Tang, S. K., Wang, Y., Cai, M., Lou, K., Mao, P. H., Jin, X.,Jiang, C. L., Xu, L. H. & Li, W. J. ( 2008; ). Microbulbifer halophilus sp. nov., a moderately halophilic bacteriumfrom north-west China. Int J Syst Evol Microbiol 58, 2036–2040.[CrossRef]
    [Google Scholar]
  27. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. &Higgins, D. G. ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignmentaided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  28. Vaskovsky, V. E. & Terekhova, T. A. ( 1979; ). HPTLC of phospholipid mixtures containing phosphatidylglycerol. J High Resolut Chromatogr 2, 671–672.[CrossRef]
    [Google Scholar]
  29. Venkateswaran, K., Moser, D. P., Dollhopf, M. E., Lies, D. P.,Saffarini, D. A., MacGregor, B. J., Ringelberg, D. B., White, D. C., Nishijima,M. & other authors ( 1999; ). Polyphasic taxonomyof the genus Shewanella and description of Shewanella oneidensis sp. nov. Int J Syst Bacteriol 49, 705–724.[CrossRef]
    [Google Scholar]
  30. Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A.D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray,R. G. E. & other authors ( 1987; ). InternationalCommittee on Systematic Bacteriology. Report of the ad hoc committee on reconciliationof approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef]
    [Google Scholar]
  31. Yoon, J.-H., Kim, I.-G., Shin, D.-Y., Kang, K. H. & Park,Y.-H. ( 2003a; ). Microbulbifer salipaludissp. nov., a moderate halophile isolated from a Korean salt marsh. Int J Syst Evol Microbiol 53, 53–57.[CrossRef]
    [Google Scholar]
  32. Yoon, J.-H., Kim, H., Kang, K. H., Oh, T.-K. & Park, Y.-H. ( 2003b; ). Transfer of Pseudomonas elongataHumm 1946 to the genus Microbulbifer as Microbulbifer elongatus comb. nov. Int J Syst Evol Microbiol 53, 1357–1361.[CrossRef]
    [Google Scholar]
  33. Yoon, J.-H., Kim, I.-G., Oh, T.-K. & Park, Y.-H. ( 2004; ). Microbulbifer maritimus sp. nov., isolatedfrom an intertidal sediment from the Yellow Sea, Korea. Int J SystEvol Microbiol 54, 1111–1116.
    [Google Scholar]
  34. Yoon, J. H., Jung, S. Y., Kang, S. J. & Oh, T. K. ( 2007; ). Microbulbifer celer sp. nov., isolated froma marine solar saltern of the Yellow Sea in Korea. Int J Syst EvolMicrobiol 57, 2365–2369.
    [Google Scholar]
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Two-dimensional TLC of polar lipids of strain KMM 3891 . PE, Phosphatidylethanolamine; PG, phosphatidylglycerol; DPG, diphosphatidylglycerol.

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Maximum-parsimony phylogenetic tree based on 16S rRNA gene sequences available from the GenBank/EMBL/DDBJ databases showing relationships of isolates KMM 3891 and KMM 3892 and related members of the . [PDF](44 KB)

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