1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 60, Issue 3

gen. nov., sp. nov., a marine gammaproteobacterium isolated from the mollusc from the Sea of Japan Free

Abstract

Two bacterial strains, KMM 3891 and KMM 3892, were isolated from internal tissues of the marine mollusc collected from the Sea of Japan. The novel isolates were Gram-negative, aerobic, faint pink–reddish-pigmented, rod-shaped, non-motile, stenohaline and psychrotolerant bacteria that were unable to degrade most tested complex polysaccharides. Polar lipids consisted of phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. Fatty acid analysis revealed C 6, C, C and C 7 as the dominant components. The major isoprenoid quinone was Q-7. The DNA G+C content of strain KMM 3891 was 51.7 mol%. According to phylogenetic analysis of 16S rRNA gene sequences, strains KMM 3891 and KMM 3892 were positioned within the as a separate branch, sharing <93 % sequence similarity to their phylogenetic relatives including , species, , and . Based on phenotypic characterization and phylogenetic distance, the novel marine isolates KMM 3891 and KMM 3892 represent a new genus and species, for which the name gen. nov., sp. nov. is proposed. The type strain of is KMM 3891 (=NRIC 0753 =JCM 15738).

  • Published Online:
SGM
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.010728-0
2010-03-01
2024-04-23
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/60/3/603.html?itemId=/content/journal/ijsem/10.1099/ijs.0.010728-0&mimeType=html&fmt=ahah

References

  1. Baumann P., Baumann L. 1981; The marine Gram-negative eubacteria: genera Photobacterium, Beneckea , Alteromonas , Pseudomonas and Alcaligenes . In The Prokaryotes vol. 1 pp 1302–1330 Edited by Starr M. P., Stolp H., Trüper H. G., Balows A., Schlegel H. Berlin: Springer;
     [Google Scholar]
  2. Blackall L. L., Hayward A. C., Sly L. I. 1985; Cellulolytic and dextranolytic gram-negative bacteria: revival of the genus Cellvibrio . J Appl Bacteriol 59:81–97 [CrossRef]
     [Google Scholar]
  3. Bligh E. G., Dyer W. J. 1959; A rapid method of total lipid extraction and purification. Can J Biochem Physiol 37:911–917
     [Google Scholar]
  4. Distel D. L., Morrill W., MacLaren-Toussaint N., Franks D., Waterbury J. 2002; Teredinibacter turnerae gen. nov., sp. nov., a dinitrogen-fixing, cellulolytic, endosymbiotic γ -proteobacterium isolated from the gills of wood-boring molluscs (Bivalvia: Teredinidae). Int J Syst Evol Microbiol 52:2261–2269 [CrossRef]
     [Google Scholar]
  5. Ekborg N. A., Gonzalez J. M., Howard M. B., Taylor L. E., Hutcheson S. W., Weiner R. M. 2005; Saccharophagus degradans gen. nov., sp. nov., versatile marine degrader of complex polysaccharides. Int J Syst Evol Microbiol 55:1545–1549 [CrossRef]
     [Google Scholar]
  6. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [CrossRef]
     [Google Scholar]
  7. Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. (editors) 1994 Methods for General and Molecular Bacteriology Washington, DC: American Society for Microbiology;
     [Google Scholar]
  8. Gonzalez J. M., Weiner R. M. 2000; Phylogenetic characterization of marine bacterium strain 2-40, a degrader of complex polysaccharides. Int J Syst Evol Microbiol 50:831–834 [CrossRef]
     [Google Scholar]
  9. Gonzalez J. M., Mayer F., Moran A., Hodson R. E., Whitman W. 1997; Microbulbifer hydrolyticus gen. nov., sp. nov., and Marinobacterium georgiense gen. nov., sp. nov., two marine bacteria from a lignin-rich pulp mill waste enrichment community. Int J Syst Bacteriol 47:369–376 [CrossRef]
     [Google Scholar]
  10. Humphry D. R., Black G. W., Cummings S. P. 2003; Reclassification of ‘ Pseudomonas fluorescens subsp. cellulosa ’ NCIMB 10462 (Ueda et al . 1952) as Cellvibrio japonicus sp. nov. and revival of Cellvibrio vulgaris sp. , nov., nom. rev. and Cellvibrio fulvus sp. nov., nom. rev. Int J Syst Evol Microbiol 53393–400 [CrossRef]
     [Google Scholar]
  11. Kurahashi M., Yokota A. 2007; Endozoicomonas elysicola gen. nov., sp. nov., a γ -proteobacterium isolated from the sea slug Elysia ornata . Syst Appl Microbiol 30:202–206 [CrossRef]
     [Google Scholar]
  12. Leifson E. 1963; Determination of carbohydrate metabolism of marine bacteria. J Bacteriol 85:1183–1184
     [Google Scholar]
  13. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5:109–118 [CrossRef]
     [Google Scholar]
  14. Mergaert J., Lednicka D., Goris J., Cnockaert M. C., De Vos P., Swings J. 2003; Taxonomic study of Cellvibrio strains and description of Cellvibrio ostraviensis sp.nov., Cellvibrio fibrivorans sp. nov. and Cellvibrio gandavensis sp. nov. Int J Syst Evol Microbiol 53:465–471 [CrossRef]
     [Google Scholar]
  15. Miyazaki M., Nogi Y., Ohta Y., Hatada Y., Fujiwara Y., Ito S., Horikoshi K. 2008; Microbulbifer agarilyticus sp. nov. and Microbulbifer thermotolerans sp. nov., agar-degrading bacteria isolated from deep-sea sediment. Int J Syst Evol Microbiol 58:1128–1133
     [Google Scholar]
  16. Owen R. J., Hill L. R., Lapage S. P. 1969; Determination of DNA base composition from melting profiles in dilute buffers. Biopolymers 7:503–516 [CrossRef]
     [Google Scholar]
  17. Pearson W. R., Lipman D. J. 1988; Improved tools for biological sequence comparison. Proc Natl Acad Sci U S A 85:2444–2448
     [Google Scholar]
  18. Romanenko L. A., Schumann P., Zhukova N. V., Rohde M., Mikhailov V. V., Stackebrandt E. 2003; Oceanisphaera litoralis gen. nov., sp. nov. a novel halophilic bacterium from marine bottom sediments. Int J Syst Evol Microbiol 53:1885–1888 [CrossRef]
     [Google Scholar]
  19. Romanenko L. A., Schumann P., Rohde M., Mikhailov V. V., Stackebrandt E. 2004; Reinekea marinisedimentorum gen. nov., sp. nov. a novel gammaproteobacterium from marine coastal sediments. Int J Syst Evol Microbiol 54:669–673 [CrossRef]
     [Google Scholar]
  20. Romanenko L. A., Uchino M., Falsen E., Frolova G. M., Zhukova N. V., Mikhailov V. V. 2005; Pseudomonas pachastrellae sp. nov., isolated from a marine sponge. Int J Syst Evol Microbiol 55:919–924
     [Google Scholar]
  21. Romanenko L. A., Uchino M., Frolova G. M., Tanaka N., Kalinovskaya N. I., Latyshev N., Mikhailov V. V. 2007; Sphingomonas molluscorum sp. nov., a novel marine isolate with antimicrobial activity. Int J Syst Evol Microbiol 57:358–363 [CrossRef]
     [Google Scholar]
  22. Shida O., Takagi H., Kadowaki K., Nakamura L. K., Komagata K. 1997; Transfer of Bacillus alginolyticus , Bacillus chondroitinus , Bacillus curdlanolyticus , Bacillus glucanolyticus , Bacillus kobensis , and Bacillus thiaminolyticus to the genus Paenibacillus and emended description of the genus Paenibacillus . Int J Syst Bacteriol 47:289–298 [CrossRef]
     [Google Scholar]
  23. Shieh W. Y., Liu T. Y., Lin S. Y., Jean W. D., Chen J. S. 2008; Simiduia agarivorans gen. nov., sp. nov. a marine, agarolytic bacterium isolated from shallow coastal water from Keelung, Taiwan. Int J Syst Evol Microbiol 58:895–900 [CrossRef]
     [Google Scholar]
  24. Smibert R. M., Krieg N. R. 1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology . pp 607–654 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
  25. Tamura K., Dudley J., Nei M., Kumar S. 2007; mega 4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24:1596–1599 [CrossRef]
     [Google Scholar]
  26. Tang S. K., Wang Y., Cai M., Lou K., Mao P. H., Jin X., Jiang C. L., Xu L. H., Li W. J. 2008; Microbulbifer halophilus sp. nov., a moderately halophilic bacterium from north-west China. Int J Syst Evol Microbiol 58:2036–2040 [CrossRef]
     [Google Scholar]
  27. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [CrossRef]
     [Google Scholar]
  28. Vaskovsky V. E., Terekhova T. A. 1979; HPTLC of phospholipid mixtures containing phosphatidylglycerol. J High Resolut Chromatogr 2:671–672 [CrossRef]
     [Google Scholar]
  29. Venkateswaran K., Moser D. P., Dollhopf M. E., Lies D. P., Saffarini D. A., MacGregor B. J., Ringelberg D. B., White D. C., Nishijima M. other authors 1999; Polyphasic taxonomy of the genus Shewanella and description of Shewanella oneidensis sp. nov. Int J Syst Bacteriol 49:705–724 [CrossRef]
     [Google Scholar]
  30. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
     [Google Scholar]
  31. Yoon J.-H., Kim I.-G., Shin D.-Y., Kang K. H., Park Y.-H. 2003a; Microbulbifer salipaludis sp. nov., a moderate halophile isolated from a Korean salt marsh. Int J Syst Evol Microbiol 53:53–57 [CrossRef]
     [Google Scholar]
  32. Yoon J.-H., Kim H., Kang K. H., Oh T.-K., Park Y.-H. 2003b; Transfer of Pseudomonas elongata Humm 1946 to the genus Microbulbifer as Microbulbifer elongatus comb. nov. Int J Syst Evol Microbiol 53:1357–1361 [CrossRef]
     [Google Scholar]
  33. Yoon J.-H., Kim I.-G., Oh T.-K., Park Y.-H. 2004; Microbulbifer maritimus sp. nov., isolated from an intertidal sediment from the Yellow Sea, Korea. Int J Syst Evol Microbiol 54:1111–1116
     [Google Scholar]
  34. Yoon J. H., Jung S. Y., Kang S. J., Oh T. K. 2007; Microbulbifer celer sp. nov., isolated from a marine solar saltern of the Yellow Sea in Korea. Int J Syst Evol Microbiol 57:2365–2369
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.010728-0
Loading
Umboniibacter marinipuniceus gen. nov., sp. nov., a marine gammaproteobacterium isolated from the mollusc Umbonium costatum from the Sea of Japan
Int J Syst Evol Microbiol 60, 603 (2010); https://doi.org/10.1099/ijs.0.010728-0
/content/journal/ijsem/10.1099/ijs.0.010728-0
/content/journal/ijsem/10.1099/ijs.0.010728-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Supplementary material 2

PDF

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error