1887

Abstract

Five heterotrophic, aerobic, catalase- and oxidase-positive, non-motile strains were characterized from freshwater habitats located in Austria, France, Uganda, P. R. China and New Zealand. The strains shared 16S rRNA gene similarities of ≥99.3 %. The novel strains grew on NSY medium over a temperature range of 10–35 °C (two strains also grew at 5 °C and one strain grew at 38 °C) and a NaCl tolerance range of 0.0–0.3 % (four strains grew up to 0.5 % NaCl). The predominant fatty acids were C, C 7, C 3-OH, and summed feature 3 (including C 7). The DNA G+C content of strain MWH-MoIso2 was 44.9 mol%. Phylogenetic analysis of 16S rRNA gene sequences demonstrated that the five new strains formed a monophyletic cluster closely related to (96–97 % sequence similarity). This cluster also harboured other isolates as well as environmental sequences which have been obtained from several habitats. Investigations with taxon-specific FISH probes demonstrated that the novel bacteria dwell as free-living, planktonic cells in freshwater systems. Based on the revealed phylogeny and pronounced chemotaxonomic differences to (presence of >7 % C 3-OH and absence of C and C 2-OH), the new strains are suggested to represent a novel species, for which the name sp. nov. is proposed. The type strain is MWH-MoIso2 (=DSM 21490=CIP 109840=LMG 25212). The novel species belongs to the minority of described species of free-living bacteria for which both data from their natural environments and culture-based knowledge are available.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.010595-0
2010-01-01
2020-01-20
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/60/1/166.html?itemId=/content/journal/ijsem/10.1099/ijs.0.010595-0&mimeType=html&fmt=ahah

References

  1. Alonso, C., Zeder, M., Piccini, C., Conde, D. & Pernthaler, J. ( 2009; ). Ecophysiological differences of betaproteobacterial populations in two hydrochemically distinct compartments of a subtropical lagoon. Environ Microbiol 11, 867–876.[CrossRef]
    [Google Scholar]
  2. Beier, S., Witzel, K. P. & Marxsen, J. ( 2008; ). Bacterial community composition in central European running waters examined by temperature gradient gel electrophoresis and sequence analysis of 16S rRNA genes. Appl Environ Microbiol 74, 188–199.[CrossRef]
    [Google Scholar]
  3. Boenigk, J., Stadler, P., Wiedlroither, A. & Hahn, M. W. ( 2004; ). Strain-specific differences in the grazing sensitivities of closely related ultramicrobacteria affiliated with the Polynucleobacter cluster. Appl Environ Microbiol 70, 5787–5793.[CrossRef]
    [Google Scholar]
  4. Crump, B. C. & Hobbie, J. E. ( 2005; ). Synchrony and seasonality in bacterioplankton communities of two temperate rivers. Limnol Oceanogr 50, 1718–1729.[CrossRef]
    [Google Scholar]
  5. Crump, B. C., Armbrust, E. V. & Baross, J. A. ( 1999; ). Phylogenetic analysis of particle-attached and free-living bacterial communities in the Columbia River, its estuary, and the adjacent coastal ocean. Appl Environ Microbiol 65, 3192–3204.
    [Google Scholar]
  6. Hahn, M. W. ( 2003; ). Isolation of strains belonging to the cosmopolitan Polynucleobacter necessarius cluster from freshwater habitats located in three climatic zones. Appl Environ Microbiol 69, 5248–5254.[CrossRef]
    [Google Scholar]
  7. Hahn, M. W. & Höfle, M. G. ( 2001; ). Grazing of protozoa and its effect on populations of aquatic bacteria. FEMS Microbiol Ecol 35, 113–121.[CrossRef]
    [Google Scholar]
  8. Hahn, M. W., Stadler, P., Wu, Q. L. & Pöckl, M. ( 2004; ). The filtration-acclimatization method for isolation of an important fraction of the not readily cultivable bacteria. J Microbiol Methods 57, 379–390.[CrossRef]
    [Google Scholar]
  9. Hahn, M. W., Pöckl, M. & Wu, Q. L. ( 2005; ). Low intraspecific diversity in a Polynucleobacter subcluster population numerically dominating bacterioplankton of a freshwater pond. Appl Environ Microbiol 71, 4539–4547.[CrossRef]
    [Google Scholar]
  10. Hahn, M. W., Lang, E., Brandt, U., Wu, Q. L. & Scheuerl, T. ( 2009; ). Emended description of the genus Polynucleobacter and the species Polynucleobacter necessarius and proposal of two subspecies, P. necessarius subsp. necessarius subsp. nov. and P. necessarius subsp. asymbioticus subsp. nov. Int J Syst Evol Microbiol 59, 2002–2009.[CrossRef]
    [Google Scholar]
  11. Heckmann, K. & Schmidt, H. J. ( 1987; ). Polynucleobacter necessarius gen. nov., sp. nov., an obligately endosymbiotic bacterium living in the cytoplasm of Euplotes aediculatus. Int J Syst Bacteriol 37, 456–457.[CrossRef]
    [Google Scholar]
  12. Kämpfer, P. & Kroppenstedt, R. M. ( 1996; ). Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 42, 989–1005.[CrossRef]
    [Google Scholar]
  13. Liao, P. C., Huang, B. H. & Huang, S. ( 2007; ). Microbial community composition of the Danshui river estuary of northern Taiwan and the practicality of the phylogenetic method in microbial barcoding. Microb Ecol 54, 497–507.[CrossRef]
    [Google Scholar]
  14. Salcher, M. M., Pernthaler, J., Zeder, M., Psenner, R. & Posch, T. ( 2008; ). Spatio-temporal niche separation of planktonic Betaproteobacteria in an oligo-mesotrophic lake. Environ Microbiol 10, 2074–2086.[CrossRef]
    [Google Scholar]
  15. Sasser, M. ( 1990; ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
  16. Shaw, A. K., Halpern, A. L., Beeson, K., Tran, B., Venter, J. C. & Martiny, J. B. ( 2008; ). It's all relative: ranking the diversity of aquatic bacterial communities. Environ Microbiol 10, 2200–2210.[CrossRef]
    [Google Scholar]
  17. Simpson, J. M., Santo Domingo, J. W. & Reasoner, D. J. ( 2004; ). Assessment of equine fecal contamination: the search for alternative bacterial source-tracking targets. FEMS Microbiol Ecol 47, 65–75.[CrossRef]
    [Google Scholar]
  18. Springer, N., Amann, R., Ludwig, W., Schleifer, K. H. & Schmidt, H. ( 1996; ). Polynucleobacter necessarius, an obligate bacterial endosymbiont of the hypotrichous ciliate Euplotes aediculatus, is a member of the beta-subclass of Proteobacteria. FEMS Microbiol Lett 135, 333–336.
    [Google Scholar]
  19. Stackebrandt, E. & Ebers, J. ( 2006; ). Taxonomic parameters revisited: tarnished gold standard. Microbiol Today 33, 152–155.
    [Google Scholar]
  20. Stamatakis, A., Hoover, P. & Rougemont, J. A. ( 2008; ). A rapid bootstrap algorithm for the RAxML web servers. Syst Biol 57, 758–771.[CrossRef]
    [Google Scholar]
  21. Tamura, K., Dudley, J., Nei, M. & Kumar, S. ( 2007; ). mega4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24, 1596–1599.[CrossRef]
    [Google Scholar]
  22. Tóth, E. M., Kéki, Z., Homonnay, Z. G., Borsodi, A. K., Márialigeti, K. & Schumann, P. ( 2008; ). Nocardioides daphniae sp. nov., isolated from Daphnia cucullata (Crustacea: Cladocera). Int J Syst Evol Microbiol 58, 78–83.[CrossRef]
    [Google Scholar]
  23. Vannini, C., Pöckl, M., Petroni, G., Wu, Q. L., Lang, E., Stackebrandt, E., Schrallhammer, M., Richardson, P. M. & Hahn, M. W. ( 2007; ). Endosymbiosis in statu nascendi: close phylogenetic relationship between obligately endosymbiotic and obligately free-living Polynucleobacter strains (Betaproteobacteria). Environ Microbiol 9, 347–359.[CrossRef]
    [Google Scholar]
  24. Watanabe, K., Komatsu, N., Ishii, Y. & Negishi, M. ( 2009; ). Effective isolation of bacterioplankton genus Polynucleobacter from freshwater environments grown on photochemically degraded dissolved organic matter. FEMS Microbiol Ecol 67, 57–68.[CrossRef]
    [Google Scholar]
  25. Wu, Q. L. & Hahn, M. W. ( 2006; ). Differences in structure and dynamics of Polynucleobacter communities in a temperate and a subtropical lake revealed at three phylogenetic levels. FEMS Microbiol Ecol 57, 67–79.[CrossRef]
    [Google Scholar]
  26. Wu, Q. L., Schauer, M., Kamst-Van Agterveld, M. P., Zwart, G. & Hahn, M. W. ( 2006; ). Bacterioplankton community composition along a salinity gradient of sixteen high-mountain lakes located on the Tibetan Plateau, China. Appl Environ Microbiol 72, 5478–5485.[CrossRef]
    [Google Scholar]
  27. Wu, Q. L., Zwart, G., Wu, J., Kamst-van Agterveld, M. P., Liu, S. & Hahn, M. W. ( 2007; ). Submersed macrophytes play a key role in structuring bacterioplankton community composition in the large, shallow, subtropical Taihu Lake, China. Environ Microbiol 9, 2765–2774.[CrossRef]
    [Google Scholar]
  28. Yakimov, M. M., Golyshin, P. N., Lang, S., Moore, E. R. W., Abraham, W.-R., Lünsdorf, H. & Timmis, K. N. ( 1998; ). Alcanivorax borkumensis gen. nov., sp. nov., a new hydrocarbon-degrading and surfactant producing marine bacterium. Int J Syst Bacteriol 48, 339–348.[CrossRef]
    [Google Scholar]
  29. Zwart, G., Crump, B. C., Kamst-van Agterveld, M. P., Hagen, F. & Han, S.-K. ( 2002; ). Typical freshwater bacteria: an analysis of available 16S rRNA gene sequences from plankton of lakes and rivers. Aquat Microb Ecol 28, 141–155.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.010595-0
Loading
/content/journal/ijsem/10.1099/ijs.0.010595-0
Loading

Data & Media loading...

Most cited articles

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error