1887

Abstract

Five heterotrophic, aerobic, catalase- and oxidase-positive, non-motile strains were characterized from freshwater habitats located in Austria, France, Uganda, P. R. China and New Zealand. The strains shared 16S rRNA gene similarities of ≥99.3 %. The novel strains grew on NSY medium over a temperature range of 10–35 °C (two strains also grew at 5 °C and one strain grew at 38 °C) and a NaCl tolerance range of 0.0–0.3 % (four strains grew up to 0.5 % NaCl). The predominant fatty acids were C, C 7, C 3-OH, and summed feature 3 (including C 7). The DNA G+C content of strain MWH-MoIso2 was 44.9 mol%. Phylogenetic analysis of 16S rRNA gene sequences demonstrated that the five new strains formed a monophyletic cluster closely related to (96–97 % sequence similarity). This cluster also harboured other isolates as well as environmental sequences which have been obtained from several habitats. Investigations with taxon-specific FISH probes demonstrated that the novel bacteria dwell as free-living, planktonic cells in freshwater systems. Based on the revealed phylogeny and pronounced chemotaxonomic differences to (presence of >7 % C 3-OH and absence of C and C 2-OH), the new strains are suggested to represent a novel species, for which the name sp. nov. is proposed. The type strain is MWH-MoIso2 (=DSM 21490=CIP 109840=LMG 25212). The novel species belongs to the minority of described species of free-living bacteria for which both data from their natural environments and culture-based knowledge are available.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.010595-0
2010-01-01
2021-03-08
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/60/1/166.html?itemId=/content/journal/ijsem/10.1099/ijs.0.010595-0&mimeType=html&fmt=ahah

References

  1. Alonso C., Zeder M., Piccini C., Conde D., Pernthaler J. 2009; Ecophysiological differences of betaproteobacterial populations in two hydrochemically distinct compartments of a subtropical lagoon. Environ Microbiol 11:867–876 [CrossRef]
    [Google Scholar]
  2. Beier S., Witzel K. P., Marxsen J. 2008; Bacterial community composition in central European running waters examined by temperature gradient gel electrophoresis and sequence analysis of 16S rRNA genes. Appl Environ Microbiol 74:188–199 [CrossRef]
    [Google Scholar]
  3. Boenigk J., Stadler P., Wiedlroither A., Hahn M. W. 2004; Strain-specific differences in the grazing sensitivities of closely related ultramicrobacteria affiliated with the Polynucleobacter cluster. Appl Environ Microbiol 70:5787–5793 [CrossRef]
    [Google Scholar]
  4. Crump B. C., Hobbie J. E. 2005; Synchrony and seasonality in bacterioplankton communities of two temperate rivers. Limnol Oceanogr 50:1718–1729 [CrossRef]
    [Google Scholar]
  5. Crump B. C., Armbrust E. V., Baross J. A. 1999; Phylogenetic analysis of particle-attached and free-living bacterial communities in the Columbia River, its estuary, and the adjacent coastal ocean. Appl Environ Microbiol 65:3192–3204
    [Google Scholar]
  6. Hahn M. W. 2003; Isolation of strains belonging to the cosmopolitan Polynucleobacter necessarius cluster from freshwater habitats located in three climatic zones. Appl Environ Microbiol 69:5248–5254 [CrossRef]
    [Google Scholar]
  7. Hahn M. W., Höfle M. G. 2001; Grazing of protozoa and its effect on populations of aquatic bacteria. FEMS Microbiol Ecol 35:113–121 [CrossRef]
    [Google Scholar]
  8. Hahn M. W., Stadler P., Wu Q. L., Pöckl M. 2004; The filtration-acclimatization method for isolation of an important fraction of the not readily cultivable bacteria. J Microbiol Methods 57:379–390 [CrossRef]
    [Google Scholar]
  9. Hahn M. W., Pöckl M., Wu Q. L. 2005; Low intraspecific diversity in a Polynucleobacter subcluster population numerically dominating bacterioplankton of a freshwater pond. Appl Environ Microbiol 71:4539–4547 [CrossRef]
    [Google Scholar]
  10. Hahn M. W., Lang E., Brandt U., Wu Q. L., Scheuerl T. 2009 Emended description of the genus Polynucleobacter and the species Polynucleobacter necessarius and proposal of two subspecies, P.necessarius subsp. necessarius subsp.nov. and P. necessarius subsp. asymbioticus subsp. nov. Int J Syst Evol Microbiol 59, 2002–2009 [CrossRef]
  11. Heckmann K., Schmidt H. J. 1987; Polynucleobacter necessarius gen. nov., sp. nov., an obligately endosymbiotic bacterium living in the cytoplasm of Euplotes aediculatus . Int J Syst Bacteriol 37:456–457 [CrossRef]
    [Google Scholar]
  12. Kämpfer P., Kroppenstedt R. M. 1996; Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 42:989–1005 [CrossRef]
    [Google Scholar]
  13. Liao P. C., Huang B. H., Huang S. 2007; Microbial community composition of the Danshui river estuary of northern Taiwan and the practicality of the phylogenetic method in microbial barcoding. Microb Ecol 54:497–507 [CrossRef]
    [Google Scholar]
  14. Salcher M. M., Pernthaler J., Zeder M., Psenner R., Posch T. 2008; Spatio-temporal niche separation of planktonic Betaproteobacteria in an oligo-mesotrophic lake. Environ Microbiol 10:2074–2086 [CrossRef]
    [Google Scholar]
  15. Sasser M. 1990 Identification of bacteria by gas chromatography of cellular fatty acids , MIDI Technical Note 101 Newark, DE: MIDI Inc;
    [Google Scholar]
  16. Shaw A. K., Halpern A. L., Beeson K., Tran B., Venter J. C., Martiny J. B. 2008; It's all relative: ranking the diversity of aquatic bacterial communities. Environ Microbiol 10:2200–2210 [CrossRef]
    [Google Scholar]
  17. Simpson J. M., Santo Domingo J. W., Reasoner D. J. 2004; Assessment of equine fecal contamination: the search for alternative bacterial source-tracking targets. FEMS Microbiol Ecol 47:65–75 [CrossRef]
    [Google Scholar]
  18. Springer N., Amann R., Ludwig W., Schleifer K. H., Schmidt H. 1996; Polynucleobacter necessarius , an obligate bacterial endosymbiont of the hypotrichous ciliate Euplotes aediculatus , is a member of the beta-subclass of Proteobacteria . FEMS Microbiol Lett 135:333–336
    [Google Scholar]
  19. Stackebrandt E., Ebers J. 2006; Taxonomic parameters revisited: tarnished gold standard. Microbiol Today 33:152–155
    [Google Scholar]
  20. Stamatakis A., Hoover P., Rougemont J. A. 2008; A rapid bootstrap algorithm for the RAxML web servers. Syst Biol 57:758–771 [CrossRef]
    [Google Scholar]
  21. Tamura K., Dudley J., Nei M., Kumar S. 2007; mega4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24:1596–1599 [CrossRef]
    [Google Scholar]
  22. Tóth E. M., Kéki Z., Homonnay Z. G., Borsodi A. K., Márialigeti K., Schumann P. 2008; Nocardioides daphniae sp. nov., isolated from Daphnia cucullata (Crustacea: Cladocera. Int J Syst Evol Microbiol 58:78–83 [CrossRef]
    [Google Scholar]
  23. Vannini C., Pöckl M., Petroni G., Wu Q. L., Lang E., Stackebrandt E., Schrallhammer M., Richardson P. M., Hahn M. W. 2007; Endosymbiosis in statu nascendi: close phylogenetic relationship between obligately endosymbiotic and obligately free-living Polynucleobacter strains ( Betaproteobacteria ). Environ Microbiol 9:347–359 [CrossRef]
    [Google Scholar]
  24. Watanabe K., Komatsu N., Ishii Y., Negishi M. 2009; Effective isolation of bacterioplankton genus Polynucleobacter from freshwater environments grown on photochemically degraded dissolved organic matter. FEMS Microbiol Ecol 67:57–68 [CrossRef]
    [Google Scholar]
  25. Wu Q. L., Hahn M. W. 2006; Differences in structure and dynamics of Polynucleobacter communities in a temperate and a subtropical lake revealed at three phylogenetic levels. FEMS Microbiol Ecol 57:67–79 [CrossRef]
    [Google Scholar]
  26. Wu Q. L., Schauer M., Kamst-Van Agterveld M. P., Zwart G., Hahn M. W. 2006; Bacterioplankton community composition along a salinity gradient of sixteen high-mountain lakes located on the Tibetan Plateau, China. Appl Environ Microbiol 72:5478–5485 [CrossRef]
    [Google Scholar]
  27. Wu Q. L., Zwart G., Wu J., Kamst-van Agterveld M. P., Liu S., Hahn M. W. 2007; Submersed macrophytes play a key role in structuring bacterioplankton community composition in the large, shallow, subtropical Taihu Lake, China. Environ Microbiol 9:2765–2774 [CrossRef]
    [Google Scholar]
  28. Yakimov M. M., Golyshin P. N., Lang S., Moore E. R. W., Abraham W.-R., Lünsdorf H., Timmis K. N. 1998; Alcanivorax borkumensis gen. nov., sp. nov., a new hydrocarbon-degrading and surfactant producing marine bacterium. Int J Syst Bacteriol 48:339–348 [CrossRef]
    [Google Scholar]
  29. Zwart G., Crump B. C., Kamst-van Agterveld M. P., Hagen F., Han S.-K. 2002; Typical freshwater bacteria: an analysis of available 16S rRNA gene sequences from plankton of lakes and rivers. Aquat Microb Ecol 28:141–155 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.010595-0
Loading
/content/journal/ijsem/10.1099/ijs.0.010595-0
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error