1887

Abstract

A novel actinomycete strain, ABO, isolated from copper-polluted sediments showed remarkable copper resistance as well as high bioaccumulation abilities. Classical taxonomic methods, including chemotaxonomy and molecular techniques, were used to characterize the isolate. Strain ABO developed a honey-yellow substrate mycelium on all ISP media tested. Abundant, white, aerial mycelium was only formed on ISP 2, 5 and 7 and MM agar. Both types of hyphae fragmented into squarish rod-shaped elements. The aerial mycelium displayed spore-like structures with smooth surfaces in long, straight to flexuous chains. The organism has a type-IV cell wall lacking mycolic acids and type-A whole-cell sugar pattern (-diaminopimelic acid, arabinose and galactose) in addition to a phospholipid type-II profile. 16S rRNA gene sequence studies indicated that this organism is a member of the family and that it forms a monophyletic clade with NT202. The DNA–DNA relatedness of strain ABO to DSM 44348 was 39.5 %. It is evident from these genotypic and phenotypic data that strain ABO represents a novel species in the genus , for which the name proposed is sp. nov. The type strain is ABO (=DSM 45259 =LMG 24814).

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2010-02-01
2019-10-21
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Electron micrograph of the reference strain DSM 44348 , grown on ISP 2 agar for 7 days at 30 °C, showing the fragmenting aerial mycelium with smooth-surfaced, spore-like structures in long, straight to flexuous chains. Bar, 1 µm.

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Extended neighbour-joining tree based on nearly complete 16S rRNA gene sequences (at least 1402 nt) showing the relationships between strain ABO and representatives of the genus . [ PDF] (55 KB)

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