An endophytic actinomycete, strain YIM 65070T, was isolated from surface-sterilized tissue of Osyris wightiana Wall. ex Wight collected from Yunnan province, south-west China, and characterized by using a polyphasic approach. Strain YIM 65070T had morphological and chemotaxonomic markers that were consistent with its classification in the genus Herbidospora. Phylogenetic analysis based on almost complete 16S rRNA gene sequences indicated that strain YIM 65070T was phylogenetically very closely related to Herbidospora cretacea IFO 15474T. DNA–DNA hybridization experiments confirmed the separate genomic status of strains YIM 65070T and H. cretacea DSM 44071T. Moreover, strain YIM 65070T could be distinguished from H. cretacea DSM 44071T by differences in several phenotypic characteristics such as tolerance to NaCl, degradation activity, utilization of sole carbon and nitrogen sources and the cellular fatty acid contents. On the basis of phenotypic and phylogenetic evidence, strain YIM 65070T was identified as a novel species of the genus Herbidospora, for which the name Herbidospora osyris sp. nov. is proposed, with YIM 65070T (=CCTCC AA 208019T=DSM 45214T) as the type strain.
Collins, M. D. & Jones, D.(1980). Lipids in the classification and identification of coryneform bacteria containing peptidoglycans based on 2,4-diaminobutyric acid. J Appl Bacteriol48, 459–470.[CrossRef][Google Scholar]
Collins, M. D., Pirouz, T., Goodfellow, M. & Minnikin, D. E.(1977). Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol100, 221–230.[CrossRef][Google Scholar]
Dong, X.-Z. & Cai, M.-Y. (editors)(2001). Determination of biochemical properties. In Manual for the Systematic Identification of General Bacteria, pp. 370–398. Beijing: Science Press (in Chinese).
Felsenstein, J.(1985). Confidence limits on phylogenies: an approach using the bootstrap. Evolution39, 783–791.[CrossRef][Google Scholar]
Goodfellow, M.(1971). Numerical taxonomy of some nocardioform bacteria. J Gen Microbiol69, 33–80.[CrossRef][Google Scholar]
Gordon, R. E., Barnett, D. A., Handerhan, J. E. & Pang, C. H.-N.(1974).Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. Int J Syst Bacteriol24, 54–63.[CrossRef][Google Scholar]
He, L., Li, W., Huang, Y., Wang, L. M., Liu, Z. H., Lanoot, B. J., Vancanneyt, M. & Swings, J.(2005).Streptomyces jietaisiensis sp. nov., isolated from soil in northern China. Int J Syst Evol Microbiol55, 1939–1944.[CrossRef][Google Scholar]
Kelly, K. L.(1964).Inter-Society Color Council – National Bureau of Standards Color-Name Charts Illustrated with Centroid Colors. Washington, DC: US Government Printing Office.
Kudo, T., Itoh, T., Miyadoh, S., Shomura, T. & Seino, A.(1993).Herbidospora gen. nov., a new genus of the family Streptosporangiaceae Goodfellow et al. 1990. Int J Syst Bacteriol43, 319–328.[CrossRef][Google Scholar]
Lechevalier, M. P., De Bièvre, C. & Lechevalier, H. A.(1977). Chemotaxonomy of aerobic actinomycetes: phospholipid composition. Biochem Syst Ecol5, 249–260.[CrossRef][Google Scholar]
Li, W. J., Xu, P., Schumann, P., Zhang, Y. Q., Pukall, R., Xu, L. H., Stackebrandt, E. & Jiang, C. L.(2007).Georgenia ruanii sp. nov., a novel actinobacterium isolated from forest soil in Yunnan (China) and emended description of the genus Georgenia. Int J Syst Evol Microbiol57, 1424–1428.[CrossRef][Google Scholar]
Li, J., Zhao, G. Z., Qin, S., Zhu, W. Y., Xu, L. H. & Li, W. J.(2009).Streptomyces sedi sp. nov., isolated from a surface-sterilized tissue of Sedum sp. Int J Syst Evol Microbiol59, 1492–1496.[CrossRef][Google Scholar]
Mesbah, M., Premachandran, U. & Whitman, W. B.(1989). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol39, 159–167.[CrossRef][Google Scholar]
Minnikin, D. E., Collins, M. D. & Goodfellow, M.(1979). Fatty acid and polar lipid composition in the classification of Cellulomonas, Oerskovia and related taxa. J Appl Bacteriol47, 87–95.[CrossRef][Google Scholar]
Petrolini, B., Quaroni, S., Sardi, P., Saracchi, M. & Andriollo, N.(1992). A sporangiate actinomycete with unusual morphological features: Streptosporangium claviforme sp. nov. Actinomycetes3, 45–50.
[Google Scholar]
Saitou, N. & Nei, M.(1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol4, 406–425.
[Google Scholar]
Sasser, M.(1990). Identification of bacteria by gas chromatography of cellular fatty acids. USFCC Newsl20, 16[Google Scholar]
Shirling, E. B. & Gottlieb, D.(1966). Methods for characterization of Streptomyces species. Int J Syst Bacteriol16, 313–340.[CrossRef][Google Scholar]
Staneck, J. L. & Roberts, G. D.(1974). Simplified approach to identification of aerobic actinomycetes by thin layer chromatography. Appl Microbiol28, 226–231.
[Google Scholar]
Tamaoka, J., Katayama-Fujimura, Y. & Kuraishi, H.(1983). Analysis of bacterial menaquinone mixtures by high performance liquid chromatography. J Appl Bacteriol54, 31–36.[CrossRef][Google Scholar]
Tamura, T. & Sakane, T.(2004).Planotetraspora silvatica sp. nov. and emended description of the genus Planotetraspora. Int J Syst Evol Microbiol54, 2053–2056.[CrossRef][Google Scholar]
Tamura, T., Suzuki, S. I. & Hatano, K.(2000).Acrocarpospora gen. nov., a new genus of the order Actinomycetales. Int J Syst Evol Microbiol50, 1163–1171.[CrossRef][Google Scholar]
Tamura, K., Dudley, J., Nei, M. & Kumar, S.(2007). MEGA4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol24, 1596–1599.[CrossRef][Google Scholar]
Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G.(1997). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res25, 4876–4882.[CrossRef][Google Scholar]
Williams, S. T., Goodfellow, M., Alderson, G., Wellington, E. M. H., Sneath, P. H. A. & Sackin, M. J.(1983). Numerical classification of Streptomyces and related genera. J Gen Microbiol129, 1743–1813.
[Google Scholar]
Xu, P., Li, W. J., Tang, S. K., Zhang, Y. Q., Chen, G. Z., Chen, H. H., Xu, L. H. & Jiang, C. L.(2005).Naxibacter alkalitolerans gen. nov., sp. nov., a novel member of the family Oxalobacteraceae isolated from China. Int J Syst Evol Microbiol55, 1149–1153.[CrossRef][Google Scholar]