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An endophytic actinomycete, strain YIM 65070T, was isolated from surface-sterilized tissue of Osyris wightiana Wall. ex Wight collected from Yunnan province, south-west China, and characterized by using a polyphasic approach. Strain YIM 65070T had morphological and chemotaxonomic markers that were consistent with its classification in the genus Herbidospora. Phylogenetic analysis based on almost complete 16S rRNA gene sequences indicated that strain YIM 65070T was phylogenetically very closely related to Herbidospora cretacea IFO 15474T. DNA–DNA hybridization experiments confirmed the separate genomic status of strains YIM 65070T and H. cretacea DSM 44071T. Moreover, strain YIM 65070T could be distinguished from H. cretacea DSM 44071T by differences in several phenotypic characteristics such as tolerance to NaCl, degradation activity, utilization of sole carbon and nitrogen sources and the cellular fatty acid contents. On the basis of phenotypic and phylogenetic evidence, strain YIM 65070T was identified as a novel species of the genus Herbidospora, for which the name Herbidospora osyris sp. nov. is proposed, with YIM 65070T (=CCTCC AA 208019T=DSM 45214T) as the type strain.
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