1887

Abstract

A Gram-positive-staining, non-motile and rod-shaped bacterium, strain ON10, was isolated from soil around a wastewater treatment plant in Korea and its taxonomic position was investigated by using a polyphasic approach. Strain ON10 grew optimally at pH 6.5–7.0 and 30 °C in the presence of 0.5 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain ON10 clustered with the clade comprising species, with which it exhibited 16S rRNA gene sequence similarity values of 98.4–98.8 %. The cell-wall peptidoglycan type was based on -diaminopimelic acid and the cell-wall sugars were glucose, mannose, arabinose and xylose. The predominant menaquinone was MK-8(H). The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, an unidentified phospholipid and an unidentified lipid. The major fatty acids were iso-C and iso-C. The DNA G+C content was 71.6 mol%. Strain ON10 exhibited DNA–DNA relatedness levels of 17–28 % to the type strains of species and could also be differentiated from these species by differences in phenotypic characteristics. On the basis of the data obtained, strain ON10 was considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is ON10 (=KCTC 19602=CCUG 57508).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.010553-0
2009-11-01
2019-12-11
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/59/11/2798.html?itemId=/content/journal/ijsem/10.1099/ijs.0.010553-0&mimeType=html&fmt=ahah

References

  1. Collins, M. D., Dorsch, M. & Stackebrandt, E. ( 1989; ). Transfer of Pimelobacter tumescens to Terrabacter gen. nov. as Terrabacter tumescens comb. nov. and of Pimelobacter jensenii to Nocardioides as Nocardioides jensenii comb. nov. Int J Syst Bacteriol 39, 1–6.[CrossRef]
    [Google Scholar]
  2. Cowan, S. T. & Steel, K. J. ( 1965; ). Manual for the Identification of Medical Bacteria. London: Cambridge University Press.
  3. Ezaki, T., Hashimoto, Y. & Yabuuchi, E. ( 1989; ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39, 224–229.[CrossRef]
    [Google Scholar]
  4. Felsenstein, J. ( 1981; ). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17, 368–376.[CrossRef]
    [Google Scholar]
  5. Felsenstein, J. ( 1993; ). phylip (phylogeny inference package), version 3.5. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle, USA.
  6. Jukes, T. H. & Cantor, C. R. ( 1969; ). Evolution of protein molecules. In Mammalian Protein Metabolism, vol. 3, pp. 21–132. Edited by H. N. Munro. New York: Academic Press.
  7. Kluge, A. G. & Farris, J. S. ( 1969; ). Quantitative phyletics and the evolution of anurans. Syst Zool 18, 1–32.[CrossRef]
    [Google Scholar]
  8. Komagata, K. & Suzuki, K. ( 1987; ). Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19, 161–207.
    [Google Scholar]
  9. Lányí, B. ( 1987; ). Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19, 1–67.
    [Google Scholar]
  10. Lee, J.-E., Seo, J. P., Lee, D. W., Ko, Y.-H. & Lee, S. D. ( 2008; ). Terrabacter lapilli sp. nov., an actinomycete isolated from stone. Int J Syst Evol Microbiol 58, 1084–1088.[CrossRef]
    [Google Scholar]
  11. Minnikin, D. E., O'Donnell, A. G., Goodfellow, M., Alderson, G., Athalye, M., Schaal, A. & Parlett, J. H. ( 1984; ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2, 233–241.[CrossRef]
    [Google Scholar]
  12. Montero-Barrientos, M., Rivas, R., Velázquez, E., Monte, E. & Roig, M. G. ( 2005; ). Terrabacter terrae sp. nov., a novel actinomycete isolated from soil in Spain. Int J Syst Evol Microbiol 55, 2491–2495.[CrossRef]
    [Google Scholar]
  13. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  14. Sasser, M. ( 1990; ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
  15. Stackebrandt, E., Rainey, F. A. & Ward-Rainey, N. L. ( 1997; ). Proposal for a new hierarchic classification system, Actinobacteria classis nov. Int J Syst Bacteriol 47, 479–491.[CrossRef]
    [Google Scholar]
  16. Tamaoka, J. & Komagata, K. ( 1984; ). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25, 125–128.[CrossRef]
    [Google Scholar]
  17. Thompson, J. D., Higgins, D. G. & Gibson, T. J. ( 1994; ). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22, 4673–4680.[CrossRef]
    [Google Scholar]
  18. Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A. D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray, R. G. E. & other authors ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef]
    [Google Scholar]
  19. Weon, H.-Y., Schumann, P., Kroppenstedt, R. M., Kim, B.-Y., Song, J., Kwon, S.-W., Go, S.-J. & Stackebrandt, E. ( 2007; ). Terrabacter aerolatus sp. nov., isolated from an air sample. Int J Syst Evol Microbiol 57, 2106–2109.[CrossRef]
    [Google Scholar]
  20. Yoon, J.-H., Kim, H., Kim, S.-B., Kim, H.-J., Kim, W. Y., Lee, S. T., Goodfellow, M. & Park, Y.-H. ( 1996; ). Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. Int J Syst Bacteriol 46, 502–505.[CrossRef]
    [Google Scholar]
  21. Yoon, J.-H., Lee, S. T. & Park, Y.-H. ( 1998; ). Inter- and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rRNA gene sequences. Int J Syst Bacteriol 48, 187–194.[CrossRef]
    [Google Scholar]
  22. Yoon, J.-H., Kang, K. H. & Park, Y.-H. ( 2003; ). Psychrobacter jeotgali sp. nov., isolated from jeotgal, a traditional Korean fermented seafood. Int J Syst Evol Microbiol 53, 449–454.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.010553-0
Loading
/content/journal/ijsem/10.1099/ijs.0.010553-0
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error