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Abstract

An aerobic, Gram-staining-negative, motile, rod-shaped bacterium, strain NH52F, was isolated from a sandy sediment sample taken from the South China Sea. On M2 agar medium (a complex medium), colonies were beige in colour. The isolate showed highest 16S rRNA gene sequence similarities to members of the genera (96.7 % similarity), (95.4–96.0 %) and (94.1 %). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain NH52F formed a distinct cluster with MB2 and LMG 24366. Optimal growth was observed at pH 7.0-8.5 and 25 °C and the new isolate required the presence of 1–4 % (w/v) NaCl. The major fatty acids were C 7, C 2-OH, C 3-OH, C 3-OH, C and 11-methyl C 7. The DNA G+C content was 60.5 mol%. The phylogenetic and chemotaxonomic characteristics of strain NH52F were similar to those of the genus . However, the differences in phenotypic properties and the 16S rRNA gene similarity values demonstrated that the new isolate differed from recognized species of the genus . On the basis of phenotypic, chemotaxonomic and phylogenetic data, this organism should be classified as a representative of a novel species in the genus , for which the name sp. nov. is proposed. The type strain is NH52F (=LMG 24841=CCTCC AB 208316=MCCC 1A04178).

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2010-02-01
2019-09-16
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vol. , part 2, pp. 275 - 280

Cellular fatty acid contents (%) of strain NH52F and recognized species of the genera , and . [ PDF] 42 KB



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