1887

Abstract

An aerobic, Gram-staining-negative, motile, rod-shaped bacterium, strain NH52F, was isolated from a sandy sediment sample taken from the South China Sea. On M2 agar medium (a complex medium), colonies were beige in colour. The isolate showed highest 16S rRNA gene sequence similarities to members of the genera (96.7 % similarity), (95.4–96.0 %) and (94.1 %). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain NH52F formed a distinct cluster with MB2 and LMG 24366. Optimal growth was observed at pH 7.0-8.5 and 25 °C and the new isolate required the presence of 1–4 % (w/v) NaCl. The major fatty acids were C 7, C 2-OH, C 3-OH, C 3-OH, C and 11-methyl C 7. The DNA G+C content was 60.5 mol%. The phylogenetic and chemotaxonomic characteristics of strain NH52F were similar to those of the genus . However, the differences in phenotypic properties and the 16S rRNA gene similarity values demonstrated that the new isolate differed from recognized species of the genus . On the basis of phenotypic, chemotaxonomic and phylogenetic data, this organism should be classified as a representative of a novel species in the genus , for which the name sp. nov. is proposed. The type strain is NH52F (=LMG 24841=CCTCC AB 208316=MCCC 1A04178).

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2010-02-01
2024-03-29
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References

  1. Ausubel F. M., Brent R., Kingston R. E., Moore D. D., Seidman J. G., Smith J. A., Struhl K. (editors) 1995 Short Protocols in Molecular Biology: a Compendium of Methods from Current Protocols in Molecular Biology , 3rd edn. New York: Wiley;
    [Google Scholar]
  2. Cowan S. T., Steel K. J. 1965 Manual for the Identification of Medical Bacteria London: Cambridge University Press;
    [Google Scholar]
  3. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [CrossRef]
    [Google Scholar]
  4. Komagata K., Suzuki K. 1987; Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19:161–207
    [Google Scholar]
  5. Labrenz M., Collins M. D., Lawson P. A., Tindall B. J., Schumann P., Hirsch P. 1999; Roseovarius tolerans gen. nov., sp. nov. a budding bacterium with variable bacteriochlorophyll a production from hypersaline Ekho Lake. Int J Syst Bacteriol 49:137–147 [CrossRef]
    [Google Scholar]
  6. Lafay B., Ruimy R., de Traubenberg C. R., Breittmayer V., Gauthier M. J., Christen R. 1995; Roseobacter algicola sp. nov., a new marine bacterium isolated from the phycosphere of the toxin-producing dinoflagellate Prorocentrum lima . Int J Syst Bacteriol 45:290–296 [CrossRef]
    [Google Scholar]
  7. Lane D. J. 1991; 16S/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics pp 115–175 Edited by Stackebrandt E., Goodfellow M. Chichester: Wiley;
    [Google Scholar]
  8. Liu C., Shao Z. 2005; Alcanivorax dieselolei sp. nov., a novel alkane-degrading bacterium isolated from sea water and deep-sea sediment. Int J Syst Evol Microbiol 55:1181–1186 [CrossRef]
    [Google Scholar]
  9. Martens T., Heidorn T., Pukall R., Simon M., Tindall B. J., Brinkhoff T. 2006; Reclassification of Roseobacter gallaeciensis Ruiz-Ponte et al. 1998 as Phaeobacter gallaeciensis gen. nov., comb. nov., description of Phaeobacter inhibens sp.nov., reclassification of Ruegeria algicola (Lafay et al. 1995)Uchino et al. 1999 as Marinovum algicola gen. nov., comb. nov., and emended descriptions of the genera Roseobacter , Ruegeria and Leisingera . Int J Syst Evol Microbiol 56:1293–1304 [CrossRef]
    [Google Scholar]
  10. Ruiz-Ponte C., Cilia V., Lambert C., Nicolas J. L. 1998; Roseobacter gallaeciensis sp. nov., a new marine bacterium isolated from rearings and collectors of the scallop Pecten maximus . Int J Syst Bacteriol 48:537–542 [CrossRef]
    [Google Scholar]
  11. Ryu E. K., MacCoss M. 1979; Modification of the Dittmer–Lester reagent for the detection of phospholipid derivatives on thin-layer chromatograms. J Lipid Res 20:561–563
    [Google Scholar]
  12. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  13. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: a Laboratory Manual , 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  14. Schaefer J. K., Goodwin K. D., McDonald I. R., Murrell J. C., Oremland R. S. 2002; Leisingera methylohalidivorans gen. nov., sp. nov. a marine methylotroph that grows on methyl bromide. Int J Syst Evol Microbiol 52:851–859 [CrossRef]
    [Google Scholar]
  15. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [CrossRef]
    [Google Scholar]
  16. Stackebrandt E., Liesack W. 1993; Nucleic acids and classification. In Handbook of New Bacterial Systematics pp 152–189 Edited by Goodfellow M., O'Donnell A. G. London: Academic Press;
    [Google Scholar]
  17. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [CrossRef]
    [Google Scholar]
  18. Vandecandelaere I., Segaert E., Mollica A., Faimali M., Vandamme P. 2008; Leisingera aquimarina sp. nov., isolated from a marine electroactive biofilm, and emended descriptions of Leisingera methylohalidivorans Schaefer et al. 2002, Phaeobacter daeponensis Yoon et al. 2007 and Phaeobacter inhibens Martens et al. 2006. Int J Syst Evol Microbiol 58:2788–2793 [CrossRef]
    [Google Scholar]
  19. Vaskovsky V. E., Kostetsky E. Y., Vasendin I. M. 1975; A universal reagent for phospholipid analysis. J Chromatogr 114:129–141 [CrossRef]
    [Google Scholar]
  20. Yoon J.-H., Kang S.-J., Lee S.-Y., Oh T.-K. 2007; Phaeobacter daeponensis sp. nov., isolated from a tidal flat of the Yellow Sea in Korea. Int J Syst Evol Microbiol 57:856–861 [CrossRef]
    [Google Scholar]
  21. Zhang D.-C., Li H.-R., Xin Y.-H., Liu H.-C., Chi Z.-M., Zhou P.-J., Yu Y. 2008; Phaeobacter arcticus sp. nov., a psychrophilic bacterium isolated from the Arctic. Int J Syst Evol Microbiol 58:1384–1387 [CrossRef]
    [Google Scholar]
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