A novel anaerobic, chemo-organotrophic bacterium, designated VNs36, was isolated from a well that collected water from a deep saline aquifer used for underground gas storage at a depth of 830 m in the Paris Basin, France. Cells were curved motile rods or vibrios (3.0–5.0×0.5 μm). Strain VNs36 grew at temperatures between 20 and 50 °C (optimum 37 °C) and at pH values between 5.0 and 9.0 (optimum 6.9). It did not require salt for growth, but tolerated up to 20 g NaCl l (optimum 2 g l). In the presence of sulfate, strain VNs36 used lactate, formate and pyruvate as carbon and energy sources. The main fermentation products from lactate were acetate, H and CO. Sulfate, thiosulfate and sulfite were used as electron acceptors, but not sulfur. The genomic DNA G+C content of strain VNs36 was 67.2 mol%. Phylogenetic analysis of the 16S rRNA gene sequence indicated that strain VNs36 was affiliated with the family within the class . On the basis of 16S rRNA gene sequence comparisons, DNA G+C content and the absence of desulfoviridin in cell extracts, it is proposed that strain VNs36 be assigned to a new genus, gen. nov., as a representative of a novel species, sp. nov. The type species of this genus is with the type strain VNs36 (=DSM 17965=JCM 14038).


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