An anaerobic acidophilic, hyperthermophilic archaeon, designated strain 345-15, was isolated from an acidic hot spring of Kamchatka (Russia). Cells of strain 345-15 were regular or irregular cocci, 1–2 μm in diameter, with flagella. Strain 345-15 grew optimally at 80–85 °C and pH 3.5–4.0 and fermented a wide range of carbohydrates, including polysaccharides. Acetate, ethanol and lactate were the fermentation products. Growth was stimulated by elemental sulfur and thiosulfate, which were reduced to hydrogen sulfide. The G+C content of the DNA was 54.5 mol%. The 16S rRNA gene sequence analysis indicated that strain 345-15 belonged to the genus . The level of DNA–DNA hybridization between strain 345-15 and 1904 was 61 %. Thus, strain 345-15 was considered as representing a novel species of the genus , with the name sp. nov. (type strain, 345-15=DSM 16705=VKM B-2471), which shared the main morphological and physiological properties of the genus but differed by the presence of flagella and the spectrum of substrates utilized. Phylogenetic analysis showed that the genus with its species sp. nov. and ‘’, and the genus , represented by and ‘’, formed a separate cluster that adjoins the cluster formed by the species of the order . Members of the cluster are thermophilic, organotrophic anaerobic cocci that can be distinguished from all species of the order on the basis of acidophily Based on these considerations, we propose a new family, fam. nov., to accommodate the subcluster of hyperthermophiles represented by the genus , a new family, fam. nov., for the subcluster of extreme thermophiles represented by the genus and a new order, ord. nov., to accommodate the two new families.


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vol. , part 12, pp. 3116 - 3122

Comparison of the characteristics of strain 345-15 ( sp. nov.) and closely related species. [PDF](21 KB)


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