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Abstract

Two Gram-staining-negative bacterial strains, designated 3A10 and ECP37, were isolated from sediment samples collected from an industrially contaminated site in northern Portugal. These two organisms were rod-shaped, non-motile, aerobic, catalase- and oxidase-positive and formed yellow colonies. The predominant fatty acids were iso-C, anteiso-C, iso-C 9 and iso-C 3-OH. The G+C content of the DNA of strains 3A10 and ECP37 was 43 and 34 mol%, respectively. The major isoprenoid quinone of the two strains was MK-6. 16S rRNA gene sequence analysis revealed that strains 3A10 and ECP37 were members of the family and were related phylogenetically to the genus . Strain 3A10 showed 16S rRNA gene sequence similarity values of 97.2 and 96.6 % to the type strains of and , respectively; strain ECP37 showed 97.3 % similarity to the type strain of . DNA–DNA hybridization experiments revealed levels of genomic relatedness of <70 % between strains 3A10 and ECP37 and between these two strains and the type strains of , and , justifying their classification as representing two novel species of the genus . The names proposed for these organisms are sp. nov. (type strain 3A10 =LMG 24685 =NBRC 104928) and sp. nov. (type strain ECP37 =LMG 24684 =NBRC 104927).

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2010-02-01
2020-11-25
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References

  1. Alexander S. K., Strete D. 2001 Microbiology: a Photographic Atlas for the Laboratory , 2nd edn. San Francisco: Benjamin-Cummings Publishing Company;
    [Google Scholar]
  2. Benson D. A., Karsch-Mizrachi I., Lipman D. J., Ostell J., Wheeler D. L. 2007; GenBank. Nucleic Acids Res 35:21–25
    [Google Scholar]
  3. Bernardet J. F., Nakagawa Y., Holmes B. 2002; Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52:1049–1070 [CrossRef]
    [Google Scholar]
  4. Bernardet J.-F., Hugo C., Bruun B. 2006; The genera Chryseobacterium and Elizabethkingia . In The Prokaryotes: a Handbook on the Biology of Bacteria . , 3rd edn. vol 7 pp 638–676 Edited by Dworkin M., Falkow S., Rosenberg E., Schleifer K. H., Stackebrandt E. New York: Springer;
  5. Carvalho M. F., Alves C. C. T., Ferreira M. I. M., De Marco P., Castro P. M. L. 2002; Isolation and initial characterization of a bacterial consortium able to mineralize fluorobenzene. Appl Environ Microbiol 68:102–105 [CrossRef]
    [Google Scholar]
  6. Cashion P., Holder-Franklin M. A., McCully J., Franklin M. 1977; A rapid method for the base ratio determination of bacterial DNA. Anal Biochem 81:461–466 [CrossRef]
    [Google Scholar]
  7. Comité de l'Antibiogramme de la Société Française de Microbiologie; 1998; Communiqué du Comité de l'Antibiogramme de la Société Française de Microbiologie. Bull Soc Fr Microbiol 13:243–258
    [Google Scholar]
  8. Costa C., Jesus-Rydin C. 2001; Site investigation on heavy metals contaminated ground in Estarreja – Portugal. Eng Geol 60:39–47 [CrossRef]
    [Google Scholar]
  9. De Ley J., Cattoir H., Reynaerts A. 1970; The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12:133–142 [CrossRef]
    [Google Scholar]
  10. Felsenstein J. 1995 phylip (phylogeny inference package) version 3.57c. Distributed by the author. Department of Genome Sciences University of Washington; Seattle, USA:
    [Google Scholar]
  11. Gomori G. 1990; Preparation of buffers. Methods Enzymol 1:138–146
    [Google Scholar]
  12. Hall T. A. 1999; BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98
    [Google Scholar]
  13. Hudson J. A., Morgan H. W., Daniel R. M. 1986; A numerical classification of some Thermus isolates. J Gen Microbiol 132:531–540
    [Google Scholar]
  14. Huß V. A. R., Festl H., Schleifer K. H. 1983; Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4:184–192 [CrossRef]
    [Google Scholar]
  15. Kämpfer P., Lodders N., Vaneechoutte M., Wauters G. 2009; Transfer of Sejongia antarctica , Sejongia jeonii , and Sejongia marina to the genus Chryseobacterium as Chryseobacterium antarcticum comb. nov., Chryseobacterium jeonii comb. nov. and Chryseobacterium marinum comb. nov. Int J Syst Evol Microbiol 59:2238–2240 [CrossRef]
    [Google Scholar]
  16. Kim M. K., Im W.-T., Shin Y. K., Lim J. H., Kim S.-H., Lee B. C., Park M.-Y., Lee K. Y., Lee S.-T. 2004; Kaistella koreensis gen. nov., sp. nov., a novel member of the Chryseobacterium Bergeyella Riemerella branch. Int J Syst Evol Microbiol 54:2319–2324 [CrossRef]
    [Google Scholar]
  17. Kuykendall L. D., Roy M. A., O'Neill J. J., Devine T. E. 1988; Fatty acids, antibiotic resistance, and deoxyribonucleic acid homology groups of Bradyrhizobium japonicum . Int J Syst Bacteriol 38:358–361 [CrossRef]
    [Google Scholar]
  18. Lane D. J. 1991; 16S/23S sequencing. In Nucleic Acid Techniques in Bacterial Systematics pp 171–204 Edited by Stackebrandt E., Goodfellow M. Chichester: Wiley;
    [Google Scholar]
  19. Lee K., Lee H. K., Choi T. H., Cho J. C. 2007; Sejongia marina sp. nov., isolated from Antarctic seawater. Int J Syst Evol Microbiol 57:2917–2921 [CrossRef]
    [Google Scholar]
  20. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [CrossRef]
    [Google Scholar]
  21. Murray R. G. E., Doetsch R. N., Robinow F. 1994; Determinative and cytological light microscopy. In Methods for General and Molecular Bacteriology . pp 21–41 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
  22. Oliveira R. S., Dodd J. C., Castro P. M. L. 2001; The mycorrhizal status of Phragmites australis in several polluted soils and sediments of an industrialised region of Northern Portugal. Mycorrhiza 10:241–247 [CrossRef]
    [Google Scholar]
  23. Rainey F. A., Ward-Rainey N., Kroppenstedt R. M., Stackebrandt E. 1996; The genus Nocardiopsis represents a phylogenetically coherent taxon and a distinct actinomycete lineage: proposal of Nocardiopsaceae fam. nov. Int J Syst Bacteriol 46:1088–1092 [CrossRef]
    [Google Scholar]
  24. Smibert R. M., Krieg N. R. 1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology . pp 611–651 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
  25. Tiago I., Teixeira I., Silva S., Chung P., Veríssimo A., Manaia C. 2004; Metabolic and genetic diversity of mesophilic and thermophilic bacteria isolated from composted municipal sludge on poly-epsilon-caprolactones. Curr Microbiol 49:407–414 [CrossRef]
    [Google Scholar]
  26. Tiago I., Pires C., Mendes V., Morais P. V., da Costa M., Veríssimo A. 2005; Microcella putealis gen. nov., sp. nov. a gram-positive alkaliphilic bacterium isolated from a nonsaline alkaline groundwater. Syst Appl Microbiol 28:479–487 [CrossRef]
    [Google Scholar]
  27. Tiago I., Mendes V., Pires C., Morais P. V., da Costa M., Veríssimo A. 2006; Chimaereicella alkaliphila gen. nov., sp. nov. a Gram-negative alkaliphilic bacterium isolated from a nonsaline alkaline groundwater. Syst Appl Microbiol 29:100–108 [CrossRef]
    [Google Scholar]
  28. Vandamme P., Bernardet J.-F., Segers P., Kersters K., Holmes B. 1994; New perspectives in the classification of the flavobacteria: description of Chryseobacterium gen.nov., Bergeyella gen. nov., and Empedobacter nom. rev. Int J Syst Bacteriol 44827–831 [CrossRef]
    [Google Scholar]
  29. Yi H., Yoon H. I., Chun J. 2005; Sejongia antarctica gen. nov., sp. nov. and Sejongia jeonii sp. nov., isolated from the Antarctic. Int J Syst Evol Microbiol 55:409–416 [CrossRef]
    [Google Scholar]
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