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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 59, Issue 11

sp. nov., a cellulolytic bacterium from Thai soil Free

Abstract

A cellulolytic bacterium, strain P2-1, isolated from soil in Thailand, was characterized using a taxonomic approach based on phenotypic and chemotaxonomic characteristics and the 16S rRNA gene sequence. The novel strain was Gram-positive, facultatively anaerobic, spore-forming and rod-shaped. It contained -diaminopimelic as the diagnostic diamino acid in the cell-wall peptidoglycan. The DNA G+C content was 52.7 mol%. The major isoprenoid quinone was MK-7. Anteiso-C and iso-C were the dominant cellular fatty acids. Phylogenetic analyses using the 16S rRNA gene sequence showed that the novel strain was affiliated to the genus . Strain P2-1 was closely related to KCTC 3998, KCTC 3910 and KCTC 13015 with 96.3–96.5 % gene sequence similarity. DNA–DNA relatedness, physiological characteristics and some biochemical characteristics clearly distinguished strain P2-1 from related species of the genus . Therefore, strain P2-1 represents a novel species of the genus for which the name sp. nov. is proposed. The type strain is P2-1 (=KCTC 13135=PCU 305=TISTR 1888).

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Keyword(s): MP, maximum-parsimony , MR-VP, methyl red/Voges–Proskauer and NJ, neighbour-joining
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2009-11-01
2025-03-24
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Paenibacillus cellulositrophicus sp. nov., a cellulolytic bacterium from Thai soil
Int J Syst Evol Microbiol 59, 2680 (2009); https://doi.org/10.1099/ijs.0.010298-0
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Cellulase activity around colonies of strain P2-1 on CMC medium flooded with Congo red.

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Neighbour-joining tree of 16S rRNA gene sequences showing the phylogenetic relationships between strain P2-1 and all recognized species of the genus . [ PDF] 70 KB

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