1887

Abstract

Two novel Gram-positive-staining, acidophilic strains were isolated from soil samples. Both show typical features of filamentous actinomycetes. On the basis of 16S rRNA gene sequence analysis, the strains are members of the family . The two strains contain hydroxydiaminopimelic acid, glycine, alanine and glutamic acid in the peptidoglycan. Fatty acid profiles clearly differentiate the two strains: cyclohexyl C, i-C and ai-C are predominant in Delta1, while the major components for Delta3 are ai-C and i-C. The two strains also differ in their major menaquinones, MK-9(H, H, H) for Delta1 and MK-9(H, H) for Delta3, and in phospholipid patterns; Delta1 displays phosphatidylglycerol, diphosphatidylglycerol, phosphatidylinositol, phosphatidylethanolamine, methyl phosphatidylethanolamine and an unknown aminophospholipid, while Delta3 also contains minor amounts of several unknown phospholipids in addition to these phospholipids. The whole-cell sugars of both strains are galactose, arabinose and xylose. The G+C content of the DNA is 72.7 mol% for Delta1 and 71.9 mol% for Delta3. On the basis of chemotaxonomic, physiological and phylogenetic data, we propose gen. nov. to accommodate the two strains, with the description of gen. nov., sp. nov. (the type species; type strain Delta1 =DSM 45227 =NBRC 104874) and sp. nov. (type strain Delta3 =DSM 45228 =NBRC 104875).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.010231-0
2009-11-01
2020-01-22
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/59/11/2752.html?itemId=/content/journal/ijsem/10.1099/ijs.0.010231-0&mimeType=html&fmt=ahah

References

  1. Aizawa, T., Ve, N. B., Kimoto, K., Iwabuchi, N., Sumida, H., Hasegawa, I., Sasaki, S., Tamura, T., Kudo, T. & other authors ( 2007; ). Curtobacterium ammoniigenes sp. nov., an ammonia-producing bacterium isolated from plants inhabiting acidic swamps in actual acid sulfate soil areas of Vietnam. Int J Syst Evol Microbiol 57, 1447–1452.[CrossRef]
    [Google Scholar]
  2. Ara, I. & Kudo, T. ( 2007a; ). Luedemannella gen. nov., a new member of the family Micromonosporaceae and description of Luedemannella helvata sp. nov. and Luedemannella flava sp. nov. J Gen Appl Microbiol 53, 39–51.[CrossRef]
    [Google Scholar]
  3. Ara, I. & Kudo, T. ( 2007b; ). Krasilnikovia gen. nov., a new member of the family Micromonosporaceae and description of Krasilnikovia cinnamonea sp. nov. Actinomycetologica 21, 1–10.[CrossRef]
    [Google Scholar]
  4. Ara, I., Matsumoto, A., Bakir, M. A., Kudo, T., Omura, S. & Takahashi, Y. ( 2008a; ). Pseudosporangium ferrugineum gen. nov., sp. nov., a new member of the family Micromonosporaceae. Int J Syst Evol Microbiol 58, 1644–1652.[CrossRef]
    [Google Scholar]
  5. Ara, I., Bakir, M. A. & Kudo, T. ( 2008b; ). Transfer of Catellatospora koreensis Lee et al. 2000 as Catelliglobosispora koreensis gen. nov., comb. nov. and Catellatospora tsunoense Asano et al. 1989 as Hamadaea tsunoense gen. nov., comb. nov., and emended description of the genus Catellatospora Asano and Kawamoto 1986 emend. Lee and Hah 2002. Int J Syst Evol Microbiol 58, 1950–1960.[CrossRef]
    [Google Scholar]
  6. Busti, E., Cavaletti, L., Monciardini, P., Schumann, P., Rohde, M., Sosio, M. & Donadio, S. ( 2006; ). Catenulispora acidiphila gen. nov., sp. nov., representative of a new evolutionary line of descent within the filamentous actinomycetes. Int J Syst Evol Microbiol 56, 1741–1746.[CrossRef]
    [Google Scholar]
  7. Cashion, P., Holder-Franklin, M. A., McCully, J. & Franklin, M. ( 1977; ). A rapid method for the base ratio determination of bacterial DNA. Anal Biochem 81, 461–466.[CrossRef]
    [Google Scholar]
  8. De Ley, J., Cattoir, H. & Reynaerts, A. ( 1970; ). The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12, 133–142.[CrossRef]
    [Google Scholar]
  9. Donadio, S., Busti, E., Monciardini, P., Bamonte, R., Mazza, P., Sosio, M. & Cavaletti, L. ( 2005; ). Sources of polyketides and nonribosomal peptides. In Biocombinatorial Approaches for Drug Finding, pp. 19–41. Edited by W. Wohlleben, T. Spellig & B. Müller-Tiemann. Berlin: Springer.
  10. Felsenstein, J. ( 2005; ). phylip (phylogeny inference package) version 3.6. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle, USA.
  11. Gauze, G. F., Preobrazhenskaya, T. P., Kudrina, E. S., Blinov, M. O., Ryabova, I. D. & Sveshnikova, M. A. ( 1957; ). Problems in the Classification of Antagonistic Actinomycetes. Moscow: State Publishing House for Medical Literature (in Russian).
  12. Huß, V. A. R., Festl, H. & Schleifer, K. H. ( 1983; ). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4, 184–192.[CrossRef]
    [Google Scholar]
  13. Jensen, P. R. & Mafnas, C. ( 2006; ). Biogeography of the marine actinomycete Salinispora. Environ Microbiol 8, 1881–1888.[CrossRef]
    [Google Scholar]
  14. Koch, C., Kroppenstedt, R. M., Rainey, F. A. & Stackebrandt, E. ( 1996; ). 16S ribosomal DNA analysis of the genera Micromonospora, Actinoplanes, Catellatospora, Catenuloplanes, Couchioplanes, Dactylosporangium, and Pilimelia and emendation of the family Micromonosporaceae. Int J Syst Bacteriol 46, 765–768.[CrossRef]
    [Google Scholar]
  15. Komagata, K. & Suzuki, K. ( 1987; ). Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19, 161–207.
    [Google Scholar]
  16. Krasil’nikov, N. A. ( 1938; ). Ray Fungi and Related Organisms – Actinomycetales. Moscow: Akademii Nauk SSSR (in Russian).
  17. MacKenzie, S. L. ( 1984; ). Amino acids and peptides. In Gas Chromatography/Mass Spectrometry Applications in Microbiology, pp. 157–204. Edited by G. Odham, L. Larsson & P.-A. Mårdh. New York: Plenum.
  18. Maerz, A. & Paul, M. R. ( 1950; ). Dictionary of Color, 2nd edn. New York: McGraw-Hill.
  19. Maldonado, L. A., Fenical, W., Jensen, P. R., Kauffman, C. A., Mincer, T. J., Ward, A. C., Bull, A. T. & Goodfellow, M. ( 2005; ). Salinispora arenicola gen. nov., sp. nov. and Salinispora tropica sp. nov., obligate marine actinomycetes belonging to the family Micromonosporaceae. Int J Syst Evol Microbiol 55, 1759–1766.[CrossRef]
    [Google Scholar]
  20. Miller, L. T. ( 1982; ). Single derivatization method for routine analysis of bacterial whole-cell fatty acids methyl esters, including hydroxy acids. J Clin Microbiol 16, 584–586.
    [Google Scholar]
  21. Monciardini, P., Cavaletti, L., Schumann, P., Rohde, M. & Donadio, S. ( 2003; ). Conexibacter woesei gen. nov., sp. nov., a novel representative of a deep evolutionary line of descent within the class Actinobacteria. Int J Syst Evol Microbiol 53, 569–576.[CrossRef]
    [Google Scholar]
  22. Qiu, F., Huang, Y., Sun, L., Zhang, X., Liu, Z. & Song, W. ( 2007; ). Leifsonia ginsengi sp. nov., isolated from ginseng root. Int J Syst Evol Microbiol 57, 405–408.[CrossRef]
    [Google Scholar]
  23. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  24. Schumann, P., Prauser, H., Rainey, F. A., Stackebrandt, E. & Hirsch, P. ( 1997; ). Friedmanniella antarctica gen. nov., sp. nov., an ll-diaminopimelic acid-containing actinomycete from Antarctic sandstone. Int J Syst Bacteriol 47, 278–283.[CrossRef]
    [Google Scholar]
  25. Shirling, E. B. & Gottlieb, D. ( 1966; ). Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16, 313–340.[CrossRef]
    [Google Scholar]
  26. Stackebrandt, E., Rainey, F. A. & Ward-Rainey, N. L. ( 1997; ). Proposal for a new hierarchic classification system, Actinobacteria classis nov. Int J Syst Bacteriol 47, 479–491.[CrossRef]
    [Google Scholar]
  27. Suter, M. A. ( 1978; ). Isolierung von Melanin-negativen Mutanten aus Streptomyces glaucescens. PhD thesis, Eidgenössische Technische Hochschule Zürich, Switzerland (no. 6276) (in German).
  28. Suzuki, K. & Komagata, K. ( 1983; ). Taxonomic significance of cellular fatty acid composition in some coryneform bacteria. Int J Syst Bacteriol 33, 188–200.[CrossRef]
    [Google Scholar]
  29. Tamura, T., Hatano, K. & Suzuki, K. ( 2006; ). A new genus of the family Micromonosporaceae, Polymorphospora gen. nov., with description of Polymorphospora rubra sp. nov. Int J Syst Evol Microbiol 56, 1959–1964.[CrossRef]
    [Google Scholar]
  30. Thawai, C., Tanasupawat, S., Itoh, T. & Kudo, T. ( 2006; ). Actinocatenispora thailandica gen. nov., sp. nov., a new member of the family Micromonosporaceae. Int J Syst Evol Microbiol 56, 1789–1794.[CrossRef]
    [Google Scholar]
  31. Uchida, K., Kudo, T., Suzuki, K. & Nakase, T. ( 1999; ). A new rapid method of glycolate test by diethyl ether extraction, which is applicable to a small amount of bacterial cells of less than one milligram. J Gen Appl Microbiol 45, 49–56.[CrossRef]
    [Google Scholar]
  32. Vaz-Moreira, I., Nobre, M. F., Ferreira, A. C., Schumann, P., Nunes, O. C. & Manaia, C. M. ( 2008; ). Humibacter albus gen. nov., sp. nov., isolated from sewage sludge compost. Int J Syst Evol Microbiol 58, 1014–1018.[CrossRef]
    [Google Scholar]
  33. Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A. D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray, R. G. E. & other authors ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef]
    [Google Scholar]
  34. Wiese, J., Jiang, Y., Tang, S., Thiel, V., Schmaljohann, R., Xu, L., Jiang, C. & Imhoff, J. F. ( 2008; ). A new member of the family Micromonosporaceae, Planosporangium flavigriseum gen. nov., sp. nov. Int J Syst Evol Microbiol 58, 1324–1331.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.010231-0
Loading
/content/journal/ijsem/10.1099/ijs.0.010231-0
Loading

Data & Media loading...

Supplements

vol. , part 11, pp. 2752 - 2758

Signature nucleotides that differentiate gen. nov. from other genera of the . [PDF](84 KB)



PDF

Most cited articles

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error