1887

Abstract

A Gram-variable-staining, motile and coccoid-, ovoid- or rod-shaped bacterium, strain ISL-25, was isolated from a marine solar saltern of the Yellow Sea, Korea, and its taxonomic position was investigated by means of a polyphasic study. Strain ISL-25 grew optimally at pH 7.0–8.0 and 30–37 °C. Strain ISL-25 contained -diaminopimelic acid as the cell-wall peptidoglycan, MK-7 as the predominant menaquinone and anteiso-C, anteiso-C and iso-C as the major fatty acids. The DNA G+C content was 49.3 mol%. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain ISL-25 belongs to the genus . The similarity values between the 16S rRNA gene sequence of strain ISL-25 and those of the type strains of the three currently recognized species were 97.6–98.3 %. Mean DNA–DNA relatedness values between strain ISL-25 and the type strains of the genus were 9–15 %. Differential phenotypic properties of strain ISL-25, together with the phylogenetic and genetic distinctiveness, revealed that this strain could be differentiated from other species. Therefore, strain ISL-25 represents a novel species within the genus , for which the name sp. nov. is proposed. The type strain is ISL-25 (=KCTC 13260=CCUG 56755).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.010215-0
2009-11-01
2020-01-21
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/59/11/2839.html?itemId=/content/journal/ijsem/10.1099/ijs.0.010215-0&mimeType=html&fmt=ahah

References

  1. Arahal, D. R., Márquez, M. C., Volcani, B. E., Schleifer, K. H. & Ventosa, A. ( 1999; ). Bacillus marismortui sp. nov., a new moderately halophilic species from the Dead Sea. Int J Syst Bacteriol 49, 521–530.[CrossRef]
    [Google Scholar]
  2. Ezaki, T., Hashimoto, Y. & Yabuuchi, E. ( 1989; ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39, 224–229.[CrossRef]
    [Google Scholar]
  3. Hao, M. V., Kocur, M. & Komagata, K. ( 1984; ). Marinococcus gen. nov., a new genus for motile cocci with meso-diaminopimelic acid in the cell wall; and Marinococcus albus sp. nov. and Marinococcus halophilus (Novitsky and Kushner) comb. nov. J Gen Appl Microbiol 30, 449–459.[CrossRef]
    [Google Scholar]
  4. Leifson, E. ( 1963; ). Determination of carbohydrate metabolism of marine bacteria. J Bacteriol 85, 1183–1184.
    [Google Scholar]
  5. Li, W.-J., Schumann, P., Zhang, Y.-Q., Chen, G.-Z., Tian, X.-P., Xu, L.-H., Stackebrandt, E. & Jiang, C.-L. ( 2005; ). Marinococcus halotolerans sp. nov., isolated from Qinghai, north-west China. Int J Syst Evol Microbiol 55, 1801–1804.[CrossRef]
    [Google Scholar]
  6. Rodriguez-Valera, F., Ruiz-Berraquero, F. & Ramos-Cormenzana, A. ( 1980; ). Isolation of extremely halophilic bacteria able to grow in defined inorganic media with single carbon sources. J Gen Microbiol 119, 535–538.
    [Google Scholar]
  7. Stackebrandt, E. & Goebel, B. M. ( 1994; ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef]
    [Google Scholar]
  8. Ventosa, A., Quesada, E., Rodriguez-Valera, F., Ruiz-Berraquero, F. & Ramos-Cormenzana, A. ( 1982; ). Numerical taxonomy of moderately halophilic Gram-negative rods. J Gen Microbiol 128, 1959–1968.
    [Google Scholar]
  9. Ventosa, A., García, M. T., Kamekura, M., Onishi, H. & Ruiz-Berraquero, F. ( 1989; ). Bacillus halophilus sp. nov., a moderately halophilic Bacillus species. Syst Appl Microbiol 12, 162–166.[CrossRef]
    [Google Scholar]
  10. Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A. D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray, R. G. E. & other authors ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef]
    [Google Scholar]
  11. Yoon, J.-H., Kim, H., Kim, S.-B., Kim, H.-J., Kim, W. Y., Lee, S. T., Goodfellow, M. & Park, Y.-H. ( 1996; ). Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. Int J Syst Bacteriol 46, 502–505.[CrossRef]
    [Google Scholar]
  12. Yoon, J.-H., Lee, S. T. & Park, Y.-H. ( 1998; ). Inter- and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rRNA gene sequences. Int J Syst Bacteriol 48, 187–194.[CrossRef]
    [Google Scholar]
  13. Yoon, J.-H., Kang, K. H. & Park, Y.-H. ( 2003; ). Psychrobacter jeotgali sp. nov., isolated from jeotgal, a traditional Korean fermented seafood. Int J Syst Evol Microbiol 53, 449–454.[CrossRef]
    [Google Scholar]
  14. Yoon, J.-H., Kang, S.-J. & Oh, T.-K. ( 2007; ). Reclassification of Marinococcus albus Hao et al. 1985 as Salimicrobium album gen. nov., comb. nov. and Bacillus halophilus Ventosa et al. 1990 as Salimicrobium halophilum comb. nov., and description of Salimicrobium luteum sp. nov. Int J Syst Evol Microbiol 57, 2406–2411.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.010215-0
Loading
/content/journal/ijsem/10.1099/ijs.0.010215-0
Loading

Data & Media loading...

Most cited articles

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error