1887

Abstract

often constitute a large fraction of the bacterioplankton in freshwater systems. Cultivation-independent methods have revealed that the so-called acI lineage frequently represents the most numerous taxon among assemblages of freshwater and even among total freshwater bacterioplankton. Bacteria affiliated with this uncultivated lineage have been detected in freshwater habitats located in various continents and climatic zones but have never been found among terrestrial or offshore marine systems. So far, this ecologically important lineage of freshwater is not represented by a recognized taxon. In this study, we established a stable mixed culture containing a strain affiliated with the acI lineage from a freshwater lake in Austria. The proportion of the strain in the culture could be increased by manipulation of the medium composition by more than one order of magnitude, however all subsequent attempts to isolate this strain into pure culture were unsuccessful. Some of the phenotypic traits of this acI strain were determined and its taxonomic position within the was analysed. Phylogenetic analysis of this organism's 16S rRNA gene revealed a distant relationship with cultivated organisms and recognized species (89 % gene sequence similarity with the latter). Furthermore, this analysis did not support a clear assignment of the strain to any of the recognized families within the phylum . It is suggested that a candidate taxon, ‘ Planktophila limnetica’ is established to represent this strain.

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2009-11-01
2019-10-20
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vol. , part 11, pp. 2864 - 2869

Cultivation of ‘ Planktophila limnetica’. Cultivation of the mixed culture containing ‘ Planktophila limnetica’. Maximum-likelihood tree calculated with 16S rRNA gene sequences. Neighbour-joining tree illustrating the phylogenetic position of ‘ Planktophila limnetica’ within the acI clade. . Sequences containing the oligonucleotide sequence 5′-GAAACTTGGTGGCATCRCCG-3′ diagnostic for the acI-AII cluster represented by ‘ Planktophila limnetica’.

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