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Abstract

A Gram-stain-positive, rod-shaped, endospore-forming, halophilic, alkalitolerant bacterium, designated halo-1, was isolated from sediment of Xiarinaoer soda lake, located in the Inner Mongolia Autonomous Region of China. Strain halo-1 grew in the presence of 9–30 % (w/v) NaCl (optimum 19 %) and at pH 5–10 (optimum pH 9). The cell-wall peptidoglycan contained -diaminopimelic acid and the major respiratory isoprenoid quinone was MK-7. The predominant cellular fatty acids of the isolate were anteiso-C (58.35 %), anteiso-C (12.89 %) and C (6.52 %). The polar lipids contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, glycolipid and a phospholipid of unknown structure. The DNA G+C content of the strain was 46.4 mol%. On the basis of 16S rRNA gene sequence similarity, strain halo-1 showed the highest similarity (93.9 %) to CH9d. Strain halo-1 could be clearly differentiated from its closest phylogenetic relative on the basis of several phenotypic, genotypic and chemotaxonomic features. Therefore, strain halo-1 represents a novel species, for which the name sp. nov. is proposed, with the type strain halo-1 (=CGMCC 1.7653 =NBRC 104934).

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2010-06-01
2020-01-27
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vol. , part 6, pp. 1339 - 1343

Maximum-parsimony and minimum-evolution trees based on 16S rRNA gene sequences of strain halo-1 and closely related species. [PDF](44 KB)



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