1887

Abstract

A novel Gram-negative, slightly halophilic, catalase-positive, oxidase-negative, obligately aerobic, non-sporulating rod-shaped bacterium, designated strain JSM 078169, was isolated from a sea urchin () collected from the South China Sea. Growth occurred with 1–20 % (w/v) total salts (optimum, 3–5 %), at pH 6.0–10.5 (optimum, pH 7.5) and at 4–40 °C (optimum, 25–30 °C). The major cellular fatty acids were C 7, C and C 3-OH. The predominant respiratory quinone was Q-9 and the genomic DNA G+C content was 55.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain JSM 078169 should be assigned to the genus . The sequence similarities between the isolate and the type strains of members of the genus were in the range 92.4–97.0 %. The combination of phylogenetic analysis, DNA–DNA relatedness, phenotypic characteristics and chemotaxonomic data supported the view that strain JSM 078169 represents a novel species of the genus , for which the name sp. nov. is proposed, with JSM 078169 (=CCTCC AB 208031=DSM 21076=KCTC 22279) as the type strain.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.010173-0
2009-11-01
2019-10-15
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/59/11/2888.html?itemId=/content/journal/ijsem/10.1099/ijs.0.010173-0&mimeType=html&fmt=ahah

References

  1. Arahal, D. R., Vreeland, R. H., Litchfield, C. D., Mormile, M. R., Tindall, B. J., Oren, A., Bejar, V., Quesada, E. & Ventosa, A. ( 2007; ). Recommended minimal standards for describing new taxa of the family Halomonadaceae. Int J Syst Evol Microbiol 57, 2436–2446.[CrossRef]
    [Google Scholar]
  2. Atlas, R. M. ( 1993; ). Handbook of Microbiological Media. Edited by L. C. Parks. Boca Raton, FL: CRC Press.
  3. Ben Ali Gam, Z., Abdelkafi, S., Casalot, L., Tholozan, J. L., Oueslati, R. & Labat, M. ( 2007; ). Modicisalibacter tunisiensis gen. nov., sp. nov., an aerobic, slightly halophilic bacterium isolated from an oilfieldwater injection sample, and emended description of the family Halomonadaceae Franzmann et al. 1989 emend Dobson and Franzmann 1996 emend. Ntougias et al. 2007. Int J Syst Evol Microbiol 57, 2307–2313.[CrossRef]
    [Google Scholar]
  4. Chen, Y.-G., Cui, X.-L., Pukall, R., Li, H.-M., Yang, Y.-L., Xu, L.-H., Wen, M.-L., Peng, Q. & Jiang, C.-L. ( 2007; ). Salinicoccus kunmingensis sp. nov., a moderately halophilic bacterium isolated from a salt mine in Yunnan, south-west China. Int J Syst Evol Microbiol 57, 2327–2332.[CrossRef]
    [Google Scholar]
  5. Chen, Y.-G., Zhang, Y.-Q., Shi, J.-X., Xiao, H.-D., Tang, S.-K., Liu, Z.-X., Huang, K., Cui, X.-L. & Li, W.-J. ( 2009a; ). Jeotgalicoccus marinus sp. nov., a marine bacterium isolated from a sea urchin. Int J Syst Evol Microbiol 59, 1625–1629.[CrossRef]
    [Google Scholar]
  6. Chen, Y.-G., Zhang, Y.-Q., Xiao, H.-D., Liu, Z.-X., Yi, L.-B., Shi, J.-X., Zhi, X.-Y., Cui, X.-L. & Li, W.-J. ( 2009b; ). Pontibacillus halophilus sp. nov., a moderately halophilic bacterium isolated from a sea urchin. Int J Syst Evol Microbiol 59, 1635–1639.[CrossRef]
    [Google Scholar]
  7. Chun, J., Lee, J.-H., Jung, Y., Kim, M., Kim, S., Kim, B. K. & Lim, Y. W. ( 2007; ). EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57, 2259–2261.[CrossRef]
    [Google Scholar]
  8. Cowan, S. T. & Steel, K. J. ( 1965; ). Manual for the Identification of Medical Bacteria. London: Cambridge University Press.
  9. Cui, X.-L., Mao, P.-H., Zeng, M., Li, W.-J., Zhang, L.-P., Xu, L.-H. & Jiang, C.-L. ( 2001; ). Streptomonospora salina gen. nov., sp. nov., a new member of the family Nocardiopsaceae. Int J Syst Evol Microbiol 51, 357–363.
    [Google Scholar]
  10. Dobson, S. J. & Franzmann, P. D. ( 1996; ). Unification of the genera Deleya (Baumann et al. 1983), Halomonas (Vreeland et al. 1980), and Halovibrio (Fendrich 1988) and the species Paracoccus halodenitrificans (Robinson and Gibbons 1952) into a single genus, Halomonas, and placement of the genus Zymobacter in the family Halomonadaceae. Int J Syst Bacteriol 46, 550–558.[CrossRef]
    [Google Scholar]
  11. Ezaki, T., Hashimoto, Y. & Yabuuchi, E. ( 1989; ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39, 224–229.[CrossRef]
    [Google Scholar]
  12. Felsenstein, J. ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  13. Felsenstein, J. ( 1997; ). An alternative least-squares approach to inferring phylogenies from pairwise distances. Syst Biol 46, 101–111.[CrossRef]
    [Google Scholar]
  14. Fendrich, C. ( 1988; ). Halovibrio variabilis gen. nov. sp. nov., Pseudomonas halophila sp. nov. and a new halophilic aerobic coccoid Eubacterium from Great Salt Lake, Utah, USA. Syst Appl Microbiol 11, 36–43.[CrossRef]
    [Google Scholar]
  15. Franzmann, P. D., Wehmeyer, U. & Stackebrandt, E. ( 1988; ). Halomonadaceae fam. nov., a new family of the class Proteobacteria to accommodate the genera Halomonas and Deleya. Syst Appl Microbiol 11, 16–19.[CrossRef]
    [Google Scholar]
  16. Gregersen, T. ( 1978; ). Rapid method for distinction of Gram-negative from Gram-positive bacteria. Eur J Appl Microbiol Biotechnol 5, 123–127.[CrossRef]
    [Google Scholar]
  17. Groth, I., Schumann, P., Weiss, N., Martin, K. & Rainey, F. A. ( 1996; ). Agrococcus jenensis gen. nov., sp. nov., a new genus of actinomycetes with diaminobutyric acid in the cell wall. Int J Syst Bacteriol 46, 234–239.[CrossRef]
    [Google Scholar]
  18. Hopwood, D. A., Bibb, M. J., Chater, K. F., Kieser, T., Bruton, C. J., Kieser, H. M., Lydiate, D. J., Smith, C. P. & Ward, J. M. ( 1985; ). Preparation of chromosomal, plasmid and phage DNA. In Genetic Manipulation of Streptomyces: a Laboratory Manual, pp. 79–80. Norwich, UK: John Innes Foundation.
  19. Kaye, J. Z., Márquez, M. C., Ventosa, A. & Baross, J. A. ( 2004; ). Halomonas neptunia sp. nov., Halomonas sulfidaeris sp. nov., Halomonas axialensis sp. nov. and Halomonas hydrothermalis sp. nov., halophilic bacteria isolated from deep-sea hydrothermal-vent environments. Int J Syst Evol Microbiol 54, 499–511.[CrossRef]
    [Google Scholar]
  20. Kimura, M. ( 1980; ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16, 111–120.[CrossRef]
    [Google Scholar]
  21. Kluge, A. G. & Farris, J. S. ( 1969; ). Quantitative phyletics and the evolution of anurans. Syst Zool 18, 1–32.[CrossRef]
    [Google Scholar]
  22. Li, H.-B., Zhang, L.-P. & Chen, S.-F. ( 2008; ). Halomonas korlensis sp. nov., a moderately halophilic, denitrifying bacterium isolated from saline and alkaline soil. Int J Syst Evol Microbiol 58, 2582–2588.[CrossRef]
    [Google Scholar]
  23. Mata, J. A., Martínez-Cánovas, J., Quesada, E. & Bèjar, V. ( 2002; ). A detailed phenotypic characterization of the type strains of Halomonas species. Syst Appl Microbiol 25, 360–375.[CrossRef]
    [Google Scholar]
  24. Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  25. Ntougias, S., Zervakis, G. I. & Fasseas, C. ( 2007; ). Halotalea alkalilenta gen. nov., sp. nov., a novel osmotolerant and alkalitolerant bacterium from alkaline olive mill wastes, and emended description of the family Halomonadaceae Franzmann et al. 1989, emend. Dobson and Franzmann 1996. Int J Syst Evol Microbiol 57, 1975–1983.[CrossRef]
    [Google Scholar]
  26. Quillaguamán, J., Hatti-Kaul, R., Mattiasson, B., Alvarez, M. T. & Delgado, O. ( 2004; ). Halomonas boliviensis sp. nov., an alkalitolerant, moderate halophile isolated from soil around a Bolivian hypersaline lake. Int J Syst Evol Microbiol 54, 721–725.[CrossRef]
    [Google Scholar]
  27. Romanenko, L. A., Schumann, P., Rohde, M., Mikhailov, V. V. & Stackebrandt, E. ( 2002; ). Halomonas halocynthiae sp. nov., isolated from the marine ascidian Halocynthia aurantium. Int J Syst Evol Microbiol 52, 1767–1772.[CrossRef]
    [Google Scholar]
  28. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  29. Sasser, M. ( 1990; ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
  30. Smibert, R. M. & Krieg, N. R. ( 1994; ). Phenotypic characterization. In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by P. Gerhardt, R. G. E. Murray, W. A. Wood & N. R. Krieg. Washington, DC: American Society for Microbiology.
  31. Stackebrandt, E. & Goebel, B. M. ( 1994; ). Taxonomic note: a place for DNA–DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef]
    [Google Scholar]
  32. Tamura, K., Dudley, J., Nei, M. & Kumar, S. ( 2007; ). mega4: molecular evolutionary genetic analysis (mega) software version 4.0. Mol Biol Evol 24, 1596–1599.[CrossRef]
    [Google Scholar]
  33. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  34. Ventosa, A., Quesada, E., Rodriguez-Valera, F., Ruiz-Berraquero, F. & Ramos-Cormenzana, A. ( 1982; ). Numerical taxonomy of moderately halophilic Gram-negative rods. J Gen Microbiol 128, 1959–1968.
    [Google Scholar]
  35. Vreeland, R. H., Litchfield, C. D., Martin, E. L. & Elliot, E. ( 1980; ). Halomonas elongata, a new genus and species of extremely salt-tolerant bacteria. Int J Syst Bacteriol 30, 485–495.[CrossRef]
    [Google Scholar]
  36. Wang, Y., Wu, Y.-H., Wang, C.-S., Xu, X.-W., Oren, A., Zhu, X.-F. & Wu, M. ( 2008; ). Halomonas salifodinae sp. nov., a halophilic bacterium isolated from a salt mine in China. Int J Syst Evol Microbiol 58, 2855–2858.[CrossRef]
    [Google Scholar]
  37. Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A. D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray, R. G. E. & other authors ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef]
    [Google Scholar]
  38. Wu, G., Wu, X.-Q., Wang, Y.-N., Chi, C.-Q., Tang, Y.-Q., Kida, K., Wu, X.-L. & Luan, Z.-K. ( 2008; ). Halomonas daqingensis sp. nov., a moderately halophilic bacterium isolated from an oilfield soil. Int J Syst Evol Microbiol 58, 2859–2865.[CrossRef]
    [Google Scholar]
  39. Xiao, H.-D., Chen, Y.-G., Liu, Z.-X., Huang, K., Li, W.-J., Cui, X.-L., Zhang, L. & Yi, L.-B. ( 2009; ). Phylogenetic diversity of cultivable bacteria associated with a sea anemone from coast of the Naozhou Island in Zhanjiang, China. Wei Sheng Wu Xue Bao 49, 246–250 (in Chinese).
    [Google Scholar]
  40. Xu, X.-W., Wu, Y.-H., Zhou, Z., Wang, C.-S., Zhou, Y.-G., Zhang, H.-B., Wang, Y. & Wu, M. ( 2007; ). Halomonas saccharevitans sp. nov., Halomonas arcis sp. nov. and Halomonas subterranea sp. nov., halophilic bacteria isolated from hypersaline environments of China. Int J Syst Bacteriol 57, 1619–1624.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.010173-0
Loading
/content/journal/ijsem/10.1099/ijs.0.010173-0
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error