1887

Abstract

Two isolates of non-alkaliphilic, extremely halophilic archaea, with very similar characteristics, were recovered from a marine solar saltern crystallizer. Cells were pleomorphic, motile and Gram-stain-negative and grew on a limited range of carbon sources, with pyruvate being the best substrate. Optimum growth occurred at 18–20 % (w/v) NaCl, pH 6.0–8.5 and 37–40 °C. Both isolates possessed typical archaeal lipids, and their 16S rRNA gene sequences were 99.8 % identical. Phylogenetic tree reconstructions indicated that they were most closely related to the haloalkaliphile (97.5 % similarity to the type strain), but the different phenotypic properties and low DNA–DNA hybridization values between DSM 2160 and the two isolates suggested that they represent a novel species within the genus . The name sp. nov. is proposed for these isolates, with the type strain being 8.8.11 (=JCM 14361 =CECT 7526 =DSM 18674). An emended description of the genus is also provided.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.010132-0
2010-05-01
2021-02-25
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/60/5/1173.html?itemId=/content/journal/ijsem/10.1099/ijs.0.010132-0&mimeType=html&fmt=ahah

References

  1. Burns D. G., Camakaris H. M., Janssen P. H., Dyall-Smith M. L. 2004a; Combined use of cultivation-dependent and cultivation-independent methods indicates that members of most haloarchaeal groups in an Australian crystallizer pond are cultivable. Appl Environ Microbiol 70:5258–5265 [CrossRef]
    [Google Scholar]
  2. Burns D. G., Camakaris H. M., Janssen P. H., Dyall-Smith M. L. 2004b; Cultivation of Walsby's square haloarchaeon. FEMS Microbiol Lett 238:469–473
    [Google Scholar]
  3. Burns D. G., Janssen P. H., Itoh T., Kamekura M., Echigo A., Dyall-Smith M. L. 2010; Halonotius pteroides gen. nov., sp. nov., an extremely halophilic archaeon recovered from a saltern crystallizer. Int J Syst Evol Microbiol 60:1196–1199 [CrossRef]
    [Google Scholar]
  4. Falb M., Pfeiffer F., Palm P., Rodewald K., Hickmann V., Tittor J., Oesterhelt D. 2005; Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis . Genome Res 15:1336–1343 [CrossRef]
    [Google Scholar]
  5. Kamekura M. 1993; Lipids of extreme halophiles. In The Biology of Halophilic Bacteria . pp 135–161 Edited by Vreeland R. H., Hochstein L. I. Boca Raton, FL: CRC Press;
  6. Ludwig W., Strunk O., Westram R., Richter L., Meier H., Yadhukumar, Buchner A., Lai T., Steppi S. other authors 2004; arb: a software environment for sequence data. Nucleic Acids Res 32:1363–1371 [CrossRef]
    [Google Scholar]
  7. Oren A., Ventosa A., Grant W. D. 1997; Proposed minimal standards for description of new taxa in the order Halobacteriales . Int J Syst Bacteriol 47:233–238 [CrossRef]
    [Google Scholar]
  8. Soliman G. S. H., Trüper H. G. 1982; Halobacterium pharaonis sp. nov., a new, extremely haloalkaliphilic archaebacterium with low magnesium requirement. Zentralbl Bakteriol Parasitenkd Infektionskr Hyg Abt 1 Orig C3:318–329
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.010132-0
Loading
/content/journal/ijsem/10.1099/ijs.0.010132-0
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error