1887

Abstract

Two isolates of non-alkaliphilic, extremely halophilic archaea, with very similar characteristics, were recovered from a marine solar saltern crystallizer. Cells were pleomorphic, motile and Gram-stain-negative and grew on a limited range of carbon sources, with pyruvate being the best substrate. Optimum growth occurred at 18–20 % (w/v) NaCl, pH 6.0–8.5 and 37–40 °C. Both isolates possessed typical archaeal lipids, and their 16S rRNA gene sequences were 99.8 % identical. Phylogenetic tree reconstructions indicated that they were most closely related to the haloalkaliphile (97.5 % similarity to the type strain), but the different phenotypic properties and low DNA–DNA hybridization values between DSM 2160 and the two isolates suggested that they represent a novel species within the genus . The name sp. nov. is proposed for these isolates, with the type strain being 8.8.11 (=JCM 14361 =CECT 7526 =DSM 18674). An emended description of the genus is also provided.

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2010-05-01
2020-01-29
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