RT Journal Article SR Electronic(1) A1 Yoon, Jung-Hoon A1 Kang, So-Jung A1 Park, Sooyeon A1 Kim, Wonyong A1 Oh, Tae-KwangYR 2009 T1 Nocardioides caeni sp. nov., isolated from wastewater JF International Journal of Systematic and Evolutionary Microbiology, VO 59 IS 11 SP 2794 OP 2797 DO https://doi.org/10.1099/ijs.0.010124-0 PB Microbiology Society, SN 1466-5034, AB A Gram-positive, non-motile, rod- or coccoid-shaped bacterial strain, designated MN8T, was isolated from sludge of domestic wastewater in Korea, and its taxonomic position was investigated by use of a polyphasic study. Strain MN8T grew optimally at pH 6.5–7.5 and 30 °C and in the presence of 0–0.5 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain MN8T fell within the cluster comprising Nocardioides species, clustering with Nocardioides simplex KCTC 9106T, Nocardioides aromaticivorans H-1T, Nocardioides kongjuensis A2-4T and Nocardioides nitrophenolicus NSP41T, with which it shared 98.4–99.0 % 16S rRNA gene sequence similarity. The chemotaxonomic properties of strain MN8T were consistent with those of the genus Nocardioides: the cell-wall peptidoglycan type was based on ll-2,6-diaminopimelic acid, the predominant menaquinone was MK-8(H4) and the major fatty acids were iso-C16 : 0 and C18 : 1 ω9c. The DNA G+C content was 71.5 mol%. Strain MN8T exhibited levels of DNA–DNA relatedness of 13–31 % to the type strains of phylogenetically related Nocardioides species and could be differentiated from these species based on differences in phenotypic characteristics. On the basis of the data presented, strain MN8T is considered to represent a novel species of the genus Nocardioides, for which the name Nocardioides caeni sp. nov. is proposed. The type strain is MN8T (=KCTC 19600T=CCUG 57506T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.010124-0