@article{mbs:/content/journal/ijsem/10.1099/ijs.0.010017-0, author = "Burns, David G. and Janssen, Peter H. and Itoh, Takashi and Kamekura, Masahiro and Echigo, Akinobu and Dyall-Smith, Mike L.", title = "Halonotius pteroides gen. nov., sp. nov., an extremely halophilic archaeon recovered from a saltern crystallizer", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2010", volume = "60", number = "5", pages = "1196-1199", doi = "https://doi.org/10.1099/ijs.0.010017-0", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.010017-0", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", abstract = "Strains 1.15.5T, 2.27.5, 5.24.4 and 6.14.5 were isolated from a solar saltern. They have flattened, rod-shaped cells and are aerobic, extremely halophilic members of the domain Archaea and family Halobacteriaceae. Cells stained Gram-negative and grew optimally in media around neutral pH and containing 20–24 % (w/v) (strains 1.15.5T and 2.27.5) or 22–24 % (w/v) (5.24.4 and 6.14.5) salts. Mg2+ was not required. The DNA G+C contents of these isolates were all close to 58 mol%, and DNA–DNA cross-hybridization showed a mean relatedness of 77 %. Their 16S rRNA gene sequences differed by no more than 1.6 % from each other. Phylogenetic tree reconstructions with other recognized members of the Halobacteriaceae indicated that they formed a distinct clade, with the closest relative being Halorubrum saccharovorum (86.6–87.6 % 16S rRNA gene sequence similarity to the type strain). The only major polar lipid of all four isolates was the sulfated diglycosyl diether lipid S-DGD-1. By phase-contrast microscopy, the long, flattened cells of these strains often displayed a ‘wing-like’ shape. The phenotypic and phylogenetic data support the placement of these isolates into a novel species in a new genus within the Halobacteriaceae, for which we propose the name Halonotius pteroides gen. nov., sp. nov. The type strain of Halonotius pteroides is 1.15.5T (=JCM 14355T =CECT 7525T =DSM 18729T), with the additional reference strains 2.27.5 (=JCM 14356 =DSM 18671), 5.24.4 (=JCM 14357 =DSM 18673) and 6.14.5 (=JCM 14358 =DSM 18692).", }