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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 60, Issue 4

sp. nov. and sp. nov., isolated from Mongolian soil Free

Abstract

Two novel actinomycetes, designated MN07-A0368 and MN07-A0371, were isolated from soil of Terelj, Töv Province, Mongolia, and subjected to phenotypic and genotypic characterization. They produced well-developed, non-fragmenting, extensively branched substrate hyphae from which oval to irregular sporangia were produced. Chemotaxonomic characteristics, i.e. cell wall diaminopimelic acid, whole-cell sugars, fatty acid components and major menaquinones, suggested that the two organisms belonged to the genus . A comparative analysis of 16S rRNA gene sequences indicated that strains MN07-A0368 and MN07-A0371 formed a distinct clade within the genus and were closely related to the type strains of: (97.7 % sequence similarity); (97.7–97.8 %); (97.6–97.9 %); (96.9–97.0 %); and (96.5–96.8 %). Strains MN07-A0368 and MN07-A0371 could be differentiated from each other and from strains of closely related species of the genus by DNA–DNA hybridization, physiological and biochemical characteristics, fatty acid composition, and whole-cell sugar components. The broad range of phenotypic and genetic characters supported the suggestion that these strains represent two novel species of the genus , for which the names sp. nov. (type strain MN07-A0368=NBRC 105298=VTCC D9-11) and sp. nov. (type strain MN07-A0371=NBRC 105297=VTCC D9-10) are proposed.

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Keyword(s): DAP, diaminopimelic acid and SEM, scanning electron microscopy
SGM
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2010-04-01
2024-04-16
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Actinoplanes toevensis sp. nov. and Actinoplanes tereljensis sp. nov., isolated from Mongolian soil
Int J Syst Evol Microbiol 60, 919 (2010); https://doi.org/10.1099/ijs.0.009944-0
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