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Abstract

A novel actinobacterial strain, MN07-A0370, was isolated from Mongolian soil and its taxonomic status was determined using a polyphasic approach. Comparative 16S rRNA gene sequence studies revealed that strain MN07-A0370 represented a novel lineage within the actinobacteria. Strain MN07-A0370 formed a distinct clade in the family and was most closely related to the members of the genera (16S rRNA gene sequence similarity, 96.2 %–96.4 %), (94.1 %) and (93.7 %). The cell-wall peptidoglycan of the novel strain contained -lysine, alanine, aspartic acid, glutamic acid and serine and represented peptidoglycan type A4. The menaquinones were MK-8(H) and MK-8(H). The polar lipids were phosphatidylglycerol, diphosphatidylglycerol and phosphatidylinositol and the whole-cell sugars were galactose, mannose, rhamnose, ribose and glucose. Mycolic acids were absent. The fatty acid profile was characterized by the presence of large amounts of saturated iso- and anteiso-branched-chain fatty acids as well as smaller amounts of saturated straight-chain and unsaturated acids. The major fatty acids were iso-C, anteiso-C, iso-C H, C 9 and C 10-methyl. The G+C content of the DNA was 68.2 mol%. On the basis of chemotaxonomic, physiological and biochemical differences from other genera of the family , strain MN07-A0370 should be classified as representing a novel species in a new genus, for which we propose the name gen. nov., sp. nov. The type strain is MN07-A0370 (=NBRC 105296=VTCC D9-09).

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2010-03-01
2021-04-19
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