1887

Abstract

A novel actinobacterial strain, MN07-A0370, was isolated from Mongolian soil and its taxonomic status was determined using a polyphasic approach. Comparative 16S rRNA gene sequence studies revealed that strain MN07-A0370 represented a novel lineage within the actinobacteria. Strain MN07-A0370 formed a distinct clade in the family and was most closely related to the members of the genera (16S rRNA gene sequence similarity, 96.2 %–96.4 %), (94.1 %) and (93.7 %). The cell-wall peptidoglycan of the novel strain contained -lysine, alanine, aspartic acid, glutamic acid and serine and represented peptidoglycan type A4. The menaquinones were MK-8(H) and MK-8(H). The polar lipids were phosphatidylglycerol, diphosphatidylglycerol and phosphatidylinositol and the whole-cell sugars were galactose, mannose, rhamnose, ribose and glucose. Mycolic acids were absent. The fatty acid profile was characterized by the presence of large amounts of saturated iso- and anteiso-branched-chain fatty acids as well as smaller amounts of saturated straight-chain and unsaturated acids. The major fatty acids were iso-C, anteiso-C, iso-C H, C 9 and C 10-methyl. The G+C content of the DNA was 68.2 mol%. On the basis of chemotaxonomic, physiological and biochemical differences from other genera of the family , strain MN07-A0370 should be classified as representing a novel species in a new genus, for which we propose the name gen. nov., sp. nov. The type strain is MN07-A0370 (=NBRC 105296=VTCC D9-09).

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2010-03-01
2019-10-20
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References

  1. Becker, K., Schumann, P., Wullenweber, J., Schulte, M., Well,H. P., Stackebrandt, E., Peters, G. & Von Eiff, C. ( 2002; ). Kytococcus schroeteri sp. nov., a novel Gram-positiveactinobacterium isolated from a human clinical source. Int J SystEvol Microbiol 52, 1609–1614.
    [Google Scholar]
  2. Cordero, M. R. & Zumalacarregui, J. M. ( 2000; ). Characterization of Micrococcaceae isolated from saltused for Spanish dry-cured ham. Lett Appl Microbiol 31, 303–306.[CrossRef]
    [Google Scholar]
  3. De la Rosa, M. C., Mohino, M. R., Mohino, M. & Mosso, M.A. ( 1990; ). Characteristics of micrococci and staphylococciisolated from semi-preserved meat products. Food Microbiol 7, 207–215.[CrossRef]
    [Google Scholar]
  4. Eck, R. V. & Dayhoff, M. O. ( 1966; ). Atlas of Protein Sequences and Structure. Silver Springs, MD:National Biomedical Research Foundation.
  5. Felsenstein, J. ( 1985; ). Confidence limitson phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  6. Groth, I., Schumann, P., Rainey, F. A., Martin, K., Schuetze,B. & Augsten, K. ( 1997a; ). Demetria terragena gen. nov., sp. nov., a new genus of actinomycetes isolated from compostsoil. Int J Syst Bacteriol 47, 1129–1133.[CrossRef]
    [Google Scholar]
  7. Groth, I., Schumann, P., Rainey, F. A., Martin, K., Schuetze,B. & Augsten, K. ( 1997b; ). Bogoriella caseilytica gen. nov., sp. nov., a new alkaliphilic coryneform bacteria from a sodalake in Africa. Int J Syst Bacteriol 47, 788–794.[CrossRef]
    [Google Scholar]
  8. Hayakawa, M. & Nonomura, H ( 1987; ).Humic acid-vitamin agar, a new medium for selective isolation of soil actinomycetes. J Ferment Technol 65, 501–509.[CrossRef]
    [Google Scholar]
  9. Hayakawa, M., Otoguro, M., Takeuchi, T., Yamazaki, T. &Iimura, Y. ( 2000; ). Application of a method incorporatingdifferential centrifugation for selective isolation of motile actinomycetesin soil and plant litter. Antonie van Leeuwenhoek 78, 171–185.[CrossRef]
    [Google Scholar]
  10. Jones, K. L. ( 1949; ). Fresh isolatesof actinomycetes in which the presence of sporogenous aerial mycelia is afluctuating characteristic. J Bacteriol 57, 141–145.
    [Google Scholar]
  11. Kocur, M., Schleifer, K. H. & Kloos, W. E. ( 1975; ). Taxonomic status of Micrococcus nishinomiyaensisOda 1935. Int J Syst Bacteriol 25, 290–293.[CrossRef]
    [Google Scholar]
  12. Lechevalier, M. P., DeBièvre, C. & Lechevalier, H.A. ( 1977; ). Chemotaxonomy of aerobic actinomycetes:phospholipid composition. Biochem Syst Ecol 5, 249–260.[CrossRef]
    [Google Scholar]
  13. Marmur, J. ( 1961; ). A procedure for theisolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3, 208–218.[CrossRef]
    [Google Scholar]
  14. Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleicacid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  15. Minnikin, D. E., O'Donnell, A. G., Goodfellow, M., Alderson,G., Athalye, M., Schaal, A. & Parlett, J. H. ( 1984; ). An integrated procedure for the extraction of bacterial isoprenoid quinonesand polar lipids. J Microbiol Methods 2, 233–241.[CrossRef]
    [Google Scholar]
  16. Norovsuren, Zh., Zenova, G. M. & Mosina, L. V. ( 2007; ). Actinomycetes in the rhizosphere of semidesert soils ofMongolia. Eurasian Soil Sci 40, 415–418.[CrossRef]
    [Google Scholar]
  17. Nozawa, Y., Sakai, N., Arai, K., Kawasaki, Y. & Harada,K. ( 2007; ). Reliable and sensitive analysis of aminoacids in the peptidoglycan of actinomycetes using the advance Marfey'smethod. J Microbiol Methods 70, 306–311.[CrossRef]
    [Google Scholar]
  18. Papamanoli, E., Kotzekidou, P., Tzanetakis, N. & Litopoulou-Tzanetaki,E. ( 2002; ). Characterization of Micrococcaceae isolated from dry fermented sausage. Food Microbiol 19, 441–449.[CrossRef]
    [Google Scholar]
  19. Pathom-aree, W., Nogi, Y., Sutcliffe, I. C., Ward, A. C., Horikoshi,K., Bull, A. T. & Goodfellow, M. ( 2006a; ). Dermacoccus abyssi sp. nov., a piezotolerant actinomycete isolated fromthe Mariana Trench. Int J Syst Evol Microbiol 56, 1233–1237.[CrossRef]
    [Google Scholar]
  20. Pathom-aree, W., Nogi, Y., Ward, A. C., Horikoshi, K., Bull,A. T. & Goodfellow, M. ( 2006b; ). Dermacoccusbarathri sp. nov. and Dermacoccus profundi sp. nov., novel actinomycetesisolated from deep-sea mud of the Mariana Trench. Int J Syst EvolMicrobiol 56, 2303–2307.
    [Google Scholar]
  21. Rzhetsky, A. & Nei, M. ( 1992; ). Asimple method for estimating and testing minimum-evolution trees. Mol Biol Evol 9, 945–967.
    [Google Scholar]
  22. Saito, H. & Miura, K. ( 1963; ). Preparationof transforming deoxyribonucleic acid by phenol treatment. BiochimBiophys Acta 72, 619–629.
    [Google Scholar]
  23. Saitou, N. & Nei, M. ( 1987; ). Theneighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  24. Schleifer, K. H. & Kandler, O. ( 1972; ). Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36, 407–477.
    [Google Scholar]
  25. Shirling, E. B. & Gottlieb, D. ( 1966; ). Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16, 313–340.[CrossRef]
    [Google Scholar]
  26. Stackebrandt, E. & Schumann, P. ( 2000; ). Description of Bogoriellaceae fam. nov., Dermacoccaceae fam. nov., Rarobacteriaceae fam. nov. and Sanguibacteraceae fam. nov. and emendation of some families of the suborder Micrococcineae. Int J Syst Evol Microbiol 50, 1279–1285.[CrossRef]
    [Google Scholar]
  27. Stackebrandt, E., Koch, C., Gvozdiak, O. & Schumann, P. ( 1995; ). Taxonomic dissection of the genus Micrococcus: Kocuria gen. nov., Nesterenkonia gen. nov., Kytococcus gen. nov., Dermacoccus gen nov., and Micrococcus Cohn 1872 gen. emend. Int J Syst Bacteriol 45, 682–692.[CrossRef]
    [Google Scholar]
  28. Staneck, J. L. & Roberts, G. D. ( 1974; ). Simplified approach to identification of aerobic actinomycetes by thin-layerchromatography. Appl Microbiol 28, 226–231.
    [Google Scholar]
  29. Tamura, T. & Hatano, K. ( 2001; ).Phylogenetic analysis of the genus Actinoplanes and transfer of Actinoplanes minutisporangius Ruan et al. 1986 and ‘Actinoplanes aurantiacus’ to Cryptosporangium minutisporangium comb. nov. and Cryptosporangium aurantiacum sp. nov. Int J Syst Evol Microbiol 51, 2119–2125.[CrossRef]
    [Google Scholar]
  30. Tamura, T., Nakagaito, Y., Nishii, T., Hasegawa, T., Stackebrandt,E. & Yokota, A. ( 1994; ). A new genus of the order Actinomycetales, Couchioplanes gen. nov., with descriptionsof Couchioplanes caeruleus (Horan and Brodsky 1986) comb.nov. and Couchioplanes caeruleus subsp. azureus subsp. nov. Int J Syst Bacteriol 44, 193–203.[CrossRef]
    [Google Scholar]
  31. Tamura, K., Nei, M. & Kumar, S. ( 2004; ). Prospects of inferring very large phylogenies by using the neighbor-joiningmethod. Proc Natl Acad Sci U S A 101, 11030–11035.[CrossRef]
    [Google Scholar]
  32. Tamura, K., Dudley, J., Nei, M. & Kumar, S. ( 2007; ). mega4: Molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24, 1596–1599.[CrossRef]
    [Google Scholar]
  33. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. &Higgins, D. G. ( 1997; ). The clustal_x Windows interface: flexible strategies for multiple sequence alignmentaided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  34. Yokota, A., Tamura, T., Hasegawa, T. & Huang, L. H. ( 1993; ). Catenuloplanes japonicus gen. nov., sp.nov., nom. rev., a new genus of the order Actinomycetales. Int J Syst Bacteriol 43, 805–812.[CrossRef]
    [Google Scholar]
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vol. , part 3, pp. 574 - 579

Cultural characteristics of strain MN07-A0370 on different agar media at 28°C and incubated for 2 weeks. [ PDF] 46 KB



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