Skip to content
1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 59, Issue 11

sp. nov., isolated from marine sediment Free

Abstract

A novel actinomycete, strain S187, was isolated from a marine sediment sample collected from Xinghai Bay, Dalian, China. Growth occurred on ISP medium 2 containing 0–9 % NaCl and at pH 6.0–9.0 and 10–45 °C. The cell wall of strain S187 contained the isomer -diaminopimelic acid as the diagnostic diamino acid. The predominant menaquinones were MK-9(H) (40.8 %), MK-9(H) (38.2 %) and MK-9(H) (8.8 %). The major fatty acids were iso-C (29.6 %), anteiso-C (14.0 %) and anteiso-C (11.6 %). Cells contained phosphatidylethanolamine, phosphatidylinositol, phosphatidylglycerol, phosphatidylinositol mannosides and one unknown phospholipid. The GC content of the genomic DNA was 72.01 mol%. The 16S rRNA gene sequence of the isolate had similarities of 98.1 and 97.5 % with those of NRRL B-8036 (=DSM 41426) and DSM 41799, respectively, showing that the novel strain should be assigned to the genus . DNA–DNA hybridizations with the two above-mentioned species showed 31.4 and 46.9 % relatedness, respectively. Moreover, the three strains differed in some physiological and biochemical properties. Thus, on the basis of phenotypic and genotypic analyses, it is proposed that strain S187 represents a novel species of the genus , for which the name sp. nov. is proposed; the type strain is S187 (=NRRL B-24674=CCTCC AA 208049=KCTC 19546).

  • Published Online:
© SGM
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.009878-0
2009-11-01
2026-01-23

Metrics

Loading full text...

Full text loading...

/deliver/fulltext/ijsem/59/11/2870.html?itemId=/content/journal/ijsem/10.1099/ijs.0.009878-0&mimeType=html&fmt=ahah

References

  1. Altschul, S. F., Madden, T. L., Schäffer, A. A., Zhang, J., Zhang, Z., Miller, W. & Lipman, D. J.(1997). Gapped blast and psi_blast: a new generation of protein database search programs. Nucleic Acids Res 25, 3389–3402.[CrossRef] [Google Scholar]
  2. Bredholdt, H., Galatenko, O. A., Engelhardt, K., Fjærvik, E., Terekhova, L. P. & Zotchev, S. B.(2007). Rare actinomycete bacteria from the shallow water sediments of the Trondheim fjord, Norway: isolation, diversity and biological activity. Environ Microbiol 9, 2756–2764.[CrossRef] [Google Scholar]
  3. Collins, M. D.(1985). Isoprenoid quinone analysis in classification and identification. In Chemical Methods in Bacterial Systematics, pp. 267–287. Edited by M. Goodfellow & D. E. Minnikin. London: Academic Press.
  4. De Ley, J., Cattoir, H. & Reynaerts, A.(1970). The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12, 133–142.[CrossRef] [Google Scholar]
  5. Felsenstein, J.(1985). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef] [Google Scholar]
  6. Hasegawa, T., Takizawa, M. & Tanida, S.(1983). A rapid analysis for chemical grouping of aerobic actinomycetes. J Gen Appl Microbiol 29, 319–322.[CrossRef] [Google Scholar]
  7. Huß, V. A. R., Festl, H. & Schleifer, K. H.(1983). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4, 184–192.[CrossRef] [Google Scholar]
  8. Jahnke, K. D.(1992).basic computer program for evaluation of spectroscopic DNA renaturation data from GILFORD SYSTEM 2600 spectrophotometer on a PC/XT/AT type personal computer. J Microbiol Methods 15, 61–73.[CrossRef] [Google Scholar]
  9. Jensen, P. R., Gontang, E., Mafnas, C., Mincer, T. J. & Fenical, W.(2005). Culturable marine actinomycete diversity from tropical Pacific Ocean sediments. Environ Microbiol 7, 1039–1048.[CrossRef] [Google Scholar]
  10. Kelly, K. L.(1964).Inter-Society Color Council – National Bureau of Standard Color Name Charts Illustrated with Centroid Colors. Washington, DC: US Government Printing Office.
  11. Kumar, S., Tamura, K., Jakobsen, I. B. & Nei, M.(2001).mega2: molecular evolutionary genetics analysis software. Bioinformatics 17, 1244–1245.[CrossRef] [Google Scholar]
  12. Lechevalier, M. P. & Lechevalier, H. A.(1970). Chemical composition as a criterion in the classification of aerobic actinomycetes. Int J Syst Bacteriol 20, 435–443.[CrossRef] [Google Scholar]
  13. Lechevalier, M. P. & Lechevalier, H. A.(1980). The chemotaxonomy of actinomycetes. In Actinomycete Taxonomy, pp. 227–291. Edited by A. Dietz & D. W. Thayer. Arlington, VA: Society for Industrial Microbiology.
  14. Lechevalier, M. P., De Bièvre, C. & Lechevalier, H. A.(1977). Chemotaxonomy of aerobic actinomycetes: phospholipid composition. Biochem Syst Ecol 5, 249–260.[CrossRef] [Google Scholar]
  15. Lee, Y. K., Kim, H. W., Liu, C. L. & Lee, H. K.(2003). A simple method for DNA extraction from marine bacteria that produce extracellular materials. J Microbiol Methods 52, 245–250.[CrossRef] [Google Scholar]
  16. Maldonado, L. A., Stach, J. E., Pathom-Aree, W., Ward, A. C., Bull, A. T. & Goodfellow, M.(2005). Diversity of cultivable actinobacteria in geographically widespread marine sediments. Antonie van Leeuwenhoek 87, 11–18.[CrossRef] [Google Scholar]
  17. Margalith, P. & Beretta, G.(1960). Rifamycin XI: taxonomic study on Streptomyces mediterranei nov. sp. Mycopathol Mycol Appl 13, 321–330.[CrossRef] [Google Scholar]
  18. Mesbah, M., Premachandran, U. & Whitman, W. B.(1989). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef] [Google Scholar]
  19. Montalvo, N. F., Mohamed, N. M., Enticknap, J. J. & Hill, R. T.(2005). Novel actinobacteria from marine sponges. Antonie van Leeuwenhoek 87, 29–36.[CrossRef] [Google Scholar]
  20. Pathom-Aree, W., Stach, J. E., Ward, A. C., Horikoshi, K., Bull, A. T. & Goodfellow, M.(2006). Diversity of actinomycetes isolated from Challenger Deep sediment (10,898 m) from the Mariana trench. Extremophiles 10, 181–189.[CrossRef] [Google Scholar]
  21. Saitou, N. & Nei, M.(1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425. [Google Scholar]
  22. Sasser, M.(1990). Identification of bacteria by gas chromatography of cellular fatty acids. USFCC Newsl 20, 16 [Google Scholar]
  23. Shirling, E. B. & Gottlieb, D.(1966). Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16, 313–340.[CrossRef] [Google Scholar]
  24. Shirling, E. B. & Gottlieb, D.(1968a). Cooperative description of type cultures of Streptomyces. II. Species descriptions from first study. Int J Syst Bacteriol 18, 69–189.[CrossRef] [Google Scholar]
  25. Shirling, E. B. & Gottlieb, D.(1968b). Cooperative description of type cultures of Streptomyces. III. Additional species descriptions from first and second studies. Int J Syst Bacteriol 18, 279–392.[CrossRef] [Google Scholar]
  26. Shirling, E. B. & Gottlieb, D.(1969). Cooperative description of type cultures of Streptomyces. IV. Species descriptions from the second, third and fourth studies. Int J Syst Bacteriol 19, 391–512.[CrossRef] [Google Scholar]
  27. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G.(1997). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef] [Google Scholar]
  28. Ward, A. C. & Bora, N.(2006). Diversity and biogeography of marine actinobacteria. Curr Opin Microbiol 9, 279–286.[CrossRef] [Google Scholar]
  29. Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A. D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray, R. G. E. & other authors(1987). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef] [Google Scholar]
  30. Williams, S. T., Goodfellow, M., Alderson, G., Wellington, E. M. H., Sneath, P. H. A. & Sackin, M. J.(1983). Numerical classification of Streptomyces and related genera. J Gen Microbiol 129, 1743–1813. [Google Scholar]
  31. Zhang, H., Lee, Y. K., Zhang, W. & Lee, H. K.(2006). Culturable actinobacteria from the marine sponge Hymeniacidon perleve: isolation and phylogenetic diversity by 16S rRNA gene-RFLP analysis. Antonie van Leeuwenhoek 90, 159–169.[CrossRef] [Google Scholar]
/content/journal/ijsem/10.1099/ijs.0.009878-0
Loading
Streptomyces xinghaiensis sp. nov., isolated from marine sediment
Int J Syst Evol Microbiol 59, 2870 (2009); https://doi.org/10.1099/ijs.0.009878-0
/content/journal/ijsem/10.1099/ijs.0.009878-0
/content/journal/ijsem/10.1099/ijs.0.009878-0
Loading

Data & Media loading...

Supplements

Scanning electron micrograph showing the spore chain morphology of strain S187 grown on ISP medium 2 for 4 weeks at 28 °C. Bar 5 µm.

IMAGE

Combined file [ PDF] 137 KB

PDF

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An error occurred
Approval was partially successful, following selected items could not be processed due to error