A novel actinomycete, strain S187, was isolated from a marine sediment sample collected from Xinghai Bay, Dalian, China. Growth occurred on ISP medium 2 containing 0–9 % NaCl and at pH 6.0–9.0 and 10–45 °C. The cell wall of strain S187 contained the isomer -diaminopimelic acid as the diagnostic diamino acid. The predominant menaquinones were MK-9(H) (40.8 %), MK-9(H) (38.2 %) and MK-9(H) (8.8 %). The major fatty acids were iso-C (29.6 %), anteiso-C (14.0 %) and anteiso-C (11.6 %). Cells contained phosphatidylethanolamine, phosphatidylinositol, phosphatidylglycerol, phosphatidylinositol mannosides and one unknown phospholipid. The GC content of the genomic DNA was 72.01 mol%. The 16S rRNA gene sequence of the isolate had similarities of 98.1 and 97.5 % with those of NRRL B-8036 (=DSM 41426) and DSM 41799, respectively, showing that the novel strain should be assigned to the genus . DNA–DNA hybridizations with the two above-mentioned species showed 31.4 and 46.9 % relatedness, respectively. Moreover, the three strains differed in some physiological and biochemical properties. Thus, on the basis of phenotypic and genotypic analyses, it is proposed that strain S187 represents a novel species of the genus , for which the name sp. nov. is proposed; the type strain is S187 (=NRRL B-24674=CCTCC AA 208049=KCTC 19546).


Article metrics loading...

Loading full text...

Full text loading...



  1. Altschul, S. F., Madden, T. L., Schäffer, A. A., Zhang, J., Zhang, Z., Miller, W. & Lipman, D. J.(1997). Gapped blast and psi_blast: a new generation of protein database search programs. Nucleic Acids Res 25, 3389–3402.[CrossRef] [Google Scholar]
  2. Bredholdt, H., Galatenko, O. A., Engelhardt, K., Fjærvik, E., Terekhova, L. P. & Zotchev, S. B.(2007). Rare actinomycete bacteria from the shallow water sediments of the Trondheim fjord, Norway: isolation, diversity and biological activity. Environ Microbiol 9, 2756–2764.[CrossRef] [Google Scholar]
  3. Collins, M. D.(1985). Isoprenoid quinone analysis in classification and identification. In Chemical Methods in Bacterial Systematics, pp. 267–287. Edited by M. Goodfellow & D. E. Minnikin. London: Academic Press.
  4. De Ley, J., Cattoir, H. & Reynaerts, A.(1970). The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12, 133–142.[CrossRef] [Google Scholar]
  5. Felsenstein, J.(1985). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef] [Google Scholar]
  6. Hasegawa, T., Takizawa, M. & Tanida, S.(1983). A rapid analysis for chemical grouping of aerobic actinomycetes. J Gen Appl Microbiol 29, 319–322.[CrossRef] [Google Scholar]
  7. Huß, V. A. R., Festl, H. & Schleifer, K. H.(1983). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4, 184–192.[CrossRef] [Google Scholar]
  8. Jahnke, K. D.(1992).basic computer program for evaluation of spectroscopic DNA renaturation data from GILFORD SYSTEM 2600 spectrophotometer on a PC/XT/AT type personal computer. J Microbiol Methods 15, 61–73.[CrossRef] [Google Scholar]
  9. Jensen, P. R., Gontang, E., Mafnas, C., Mincer, T. J. & Fenical, W.(2005). Culturable marine actinomycete diversity from tropical Pacific Ocean sediments. Environ Microbiol 7, 1039–1048.[CrossRef] [Google Scholar]
  10. Kelly, K. L.(1964).Inter-Society Color Council – National Bureau of Standard Color Name Charts Illustrated with Centroid Colors. Washington, DC: US Government Printing Office.
  11. Kumar, S., Tamura, K., Jakobsen, I. B. & Nei, M.(2001).mega2: molecular evolutionary genetics analysis software. Bioinformatics 17, 1244–1245.[CrossRef] [Google Scholar]
  12. Lechevalier, M. P. & Lechevalier, H. A.(1970). Chemical composition as a criterion in the classification of aerobic actinomycetes. Int J Syst Bacteriol 20, 435–443.[CrossRef] [Google Scholar]
  13. Lechevalier, M. P. & Lechevalier, H. A.(1980). The chemotaxonomy of actinomycetes. In Actinomycete Taxonomy, pp. 227–291. Edited by A. Dietz & D. W. Thayer. Arlington, VA: Society for Industrial Microbiology.
  14. Lechevalier, M. P., De Bièvre, C. & Lechevalier, H. A.(1977). Chemotaxonomy of aerobic actinomycetes: phospholipid composition. Biochem Syst Ecol 5, 249–260.[CrossRef] [Google Scholar]
  15. Lee, Y. K., Kim, H. W., Liu, C. L. & Lee, H. K.(2003). A simple method for DNA extraction from marine bacteria that produce extracellular materials. J Microbiol Methods 52, 245–250.[CrossRef] [Google Scholar]
  16. Maldonado, L. A., Stach, J. E., Pathom-Aree, W., Ward, A. C., Bull, A. T. & Goodfellow, M.(2005). Diversity of cultivable actinobacteria in geographically widespread marine sediments. Antonie van Leeuwenhoek 87, 11–18.[CrossRef] [Google Scholar]
  17. Margalith, P. & Beretta, G.(1960). Rifamycin XI: taxonomic study on Streptomyces mediterranei nov. sp. Mycopathol Mycol Appl 13, 321–330.[CrossRef] [Google Scholar]
  18. Mesbah, M., Premachandran, U. & Whitman, W. B.(1989). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef] [Google Scholar]
  19. Montalvo, N. F., Mohamed, N. M., Enticknap, J. J. & Hill, R. T.(2005). Novel actinobacteria from marine sponges. Antonie van Leeuwenhoek 87, 29–36.[CrossRef] [Google Scholar]
  20. Pathom-Aree, W., Stach, J. E., Ward, A. C., Horikoshi, K., Bull, A. T. & Goodfellow, M.(2006). Diversity of actinomycetes isolated from Challenger Deep sediment (10,898 m) from the Mariana trench. Extremophiles 10, 181–189.[CrossRef] [Google Scholar]
  21. Saitou, N. & Nei, M.(1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425. [Google Scholar]
  22. Sasser, M.(1990). Identification of bacteria by gas chromatography of cellular fatty acids. USFCC Newsl 20, 16 [Google Scholar]
  23. Shirling, E. B. & Gottlieb, D.(1966). Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16, 313–340.[CrossRef] [Google Scholar]
  24. Shirling, E. B. & Gottlieb, D.(1968a). Cooperative description of type cultures of Streptomyces. II. Species descriptions from first study. Int J Syst Bacteriol 18, 69–189.[CrossRef] [Google Scholar]
  25. Shirling, E. B. & Gottlieb, D.(1968b). Cooperative description of type cultures of Streptomyces. III. Additional species descriptions from first and second studies. Int J Syst Bacteriol 18, 279–392.[CrossRef] [Google Scholar]
  26. Shirling, E. B. & Gottlieb, D.(1969). Cooperative description of type cultures of Streptomyces. IV. Species descriptions from the second, third and fourth studies. Int J Syst Bacteriol 19, 391–512.[CrossRef] [Google Scholar]
  27. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G.(1997). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef] [Google Scholar]
  28. Ward, A. C. & Bora, N.(2006). Diversity and biogeography of marine actinobacteria. Curr Opin Microbiol 9, 279–286.[CrossRef] [Google Scholar]
  29. Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A. D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray, R. G. E. & other authors(1987). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef] [Google Scholar]
  30. Williams, S. T., Goodfellow, M., Alderson, G., Wellington, E. M. H., Sneath, P. H. A. & Sackin, M. J.(1983). Numerical classification of Streptomyces and related genera. J Gen Microbiol 129, 1743–1813. [Google Scholar]
  31. Zhang, H., Lee, Y. K., Zhang, W. & Lee, H. K.(2006). Culturable actinobacteria from the marine sponge Hymeniacidon perleve: isolation and phylogenetic diversity by 16S rRNA gene-RFLP analysis. Antonie van Leeuwenhoek 90, 159–169.[CrossRef] [Google Scholar]

Data & Media loading...


Scanning electron micrograph showing the spore chain morphology of strain S187 grown on ISP medium 2 for 4 weeks at 28 °C. Bar 5 µm.


Combined file [ PDF] 137 KB


Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error