1887

Abstract

A novel actinomycete, strain S187, was isolated from a marine sediment sample collected from Xinghai Bay, Dalian, China. Growth occurred on ISP medium 2 containing 0–9 % NaCl and at pH 6.0–9.0 and 10–45 °C. The cell wall of strain S187 contained the isomer -diaminopimelic acid as the diagnostic diamino acid. The predominant menaquinones were MK-9(H) (40.8 %), MK-9(H) (38.2 %) and MK-9(H) (8.8 %). The major fatty acids were iso-C (29.6 %), anteiso-C (14.0 %) and anteiso-C (11.6 %). Cells contained phosphatidylethanolamine, phosphatidylinositol, phosphatidylglycerol, phosphatidylinositol mannosides and one unknown phospholipid. The GC content of the genomic DNA was 72.01 mol%. The 16S rRNA gene sequence of the isolate had similarities of 98.1 and 97.5 % with those of NRRL B-8036 (=DSM 41426) and DSM 41799, respectively, showing that the novel strain should be assigned to the genus . DNA–DNA hybridizations with the two above-mentioned species showed 31.4 and 46.9 % relatedness, respectively. Moreover, the three strains differed in some physiological and biochemical properties. Thus, on the basis of phenotypic and genotypic analyses, it is proposed that strain S187 represents a novel species of the genus , for which the name sp. nov. is proposed; the type strain is S187 (=NRRL B-24674=CCTCC AA 208049=KCTC 19546).

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2009-11-01
2019-10-14
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Scanning electron micrograph showing the spore chain morphology of strain S187 grown on ISP medium 2 for 4 weeks at 28 °C. Bar 5 µm.

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