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Abstract

A Gram-variable, motile, endospore-forming and rod-shaped bacterial strain, ISL-24, was isolated from a marine solar saltern of the Yellow Sea, Korea, and its taxonomic position was investigated by a polyphasic study. Strain ISL-24 grew optimally at pH 7.0–8.0, at 30–37 °C and in the presence of 8 % (w/v) NaCl. It contained MK-7 as the predominant menaquinone and anteiso-C as the predominant fatty acid. The DNA G+C content was 37.6 mol%. A phylogenetic analysis based on 16S rRNA gene sequences showed that strain ISL-24 fell within the genus , clustering with LMG 20964 and LMG 19488, with a bootstrap resampling value of 92.3 %, and exhibiting 97.3 and 97.4 % 16S rRNA gene sequence similarity, respectively, to these strains. Strain ISL-24 exhibited 94.8–96.8 % 16S rRNA gene sequence similarity to the type strains of the other species. Mean DNA–DNA relatedness values between strain ISL-24 and . DSM 14868 and . DSM 14866 were 11 and 19 %, respectively. Differential phenotypic properties of strain ISL-24, together with the phylogenetic and genetic distinctiveness, revealed that this strain is different from recognized species. On the basis of phenotypic, phylogenetic and genetic data, strain ISL-24 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is ISL-24 (=KCTC 13259 =CCUG 56754).

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2010-02-01
2020-01-28
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vol. , part 2, pp. 291 - 295

Differential phenotypic characteristics of strain ISL-24 and other species. [PDF](100 KB)



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