1887

Abstract

A novel betaproteobacterium, designated strain Ch07, was isolated from granules from the wastewater-treatment bioreactor of an alcohol fermentation factory in South Korea. In order to determine its taxonomic position, the novel strain was characterized using a polyphasic approach. The new strain was Gram-negative, facultatively anaerobic, non-spore-forming, motile and short rod-shaped. 16S rRNA gene sequence analysis revealed that strain Ch07 belonged to the class , being related to BN9 (gene sequence similarity 97.30 %), subsp. DSM 10346 (97.09 %), DSM 5571 (97.01 %), DSM 13608 (96.68 %) and DSM 1214 (96.47 %). The results of DNA–DNA hybridization tests showed that reassociation values were less than 62 % with respect to these closely related type strains. Chemotaxonomic data showed that strain Ch07 possessed ubiquinone Q-8. The G+C content of the genomic DNA was 67.9±0.1 mol%. The major polyamine of strain Ch07 was putrescine. The major polar lipids of strain Ch07 were phosphatidylethanolamine, followed by diphosphatidylglycerol and phosphatidylglycerol. When strain Ch07 was incubated on tryptic soy agar, the major cellular fatty acids were C, C cyclo, summed feature 3 (C 7/iso C 2-OH) and summed feature 5 (C 7/9/12). The results of DNA–DNA hybridizations, in combination with the chemotaxonomic and physiological data, demonstrated that strain Ch07 represents a novel species of a new genus, for which the name gen. nov., sp. nov. is proposed. The type strain of the type species is Ch07 (=KCTC 12668=LMG 24012).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.009720-0
2010-06-01
2024-10-03
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/60/6/1401.html?itemId=/content/journal/ijsem/10.1099/ijs.0.009720-0&mimeType=html&fmt=ahah

References

  1. Bligh E. G., Dyer W. J. 1959; A rapid method of total lipid extraction and purification. Can J Biochem Physiol 37:911–917 [CrossRef]
    [Google Scholar]
  2. Buck J. D. 1982; Nonstaining (KOH) method for determination of Gram reactions of marine bacteria. Appl Environ Microbiol 44:992–993
    [Google Scholar]
  3. Busse H.-J., Auling G. 1988; Polyamine pattern as a chemotaxonomic marker within the Proteobacteria . Syst Appl Microbiol 11:1–8 [CrossRef]
    [Google Scholar]
  4. Busse J., Auling G. 1992; The genera Alcaligenes and ‘ Achromobacter ’. In The Prokaryotes , 2nd edn. pp 2544–2555 Edited by Balows A., Trüper H. G., Dworkin M., Harder W., Schleifer K.-H. New York: Springer;
    [Google Scholar]
  5. Busse H.-J., Stolz A. 2006; Achromobacter, Alcaligenes and related genera. In The Prokaryotes: a Handbook on the Biology of Bacteria , 3rd edn. pp 675–700 Edited by Dworkin M., Falkow S., Rosenberg E., Schleifer K. H., Stackebrandt E. New York: Springer;
    [Google Scholar]
  6. Cappuccino J. G., Sherman N. 2002 Microbiology: a laboratory manual , 6th edn. Pearson Education, Inc; Benjamin Cummings, CA:
    [Google Scholar]
  7. Chun J., Lee J.-H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y. W. 2007; EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57:2259–2261 [CrossRef]
    [Google Scholar]
  8. Collins M. D., Jones D. 1981; Distribution of isoprenoid quinone structural types in bacteria and their taxonomic implications. Microbiol Rev 45:316–354
    [Google Scholar]
  9. De Ley J., Segers P., Kersters K., Mannheim W., Lievens A. 1986; Intra- and intergeneric similarities of the Bordetella ribosomal ribonucleic acid cistrons: proposal for a new family, Alcaligenaceae . Int J Syst Bacteriol 36:405–414 [CrossRef]
    [Google Scholar]
  10. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [CrossRef]
    [Google Scholar]
  11. Felsenstein J. 1995 phylip (Phylogeny Inference Package) and manual, version 3.57c Department of Genetics, University of Washington; Seattle, WA, USA:
    [Google Scholar]
  12. Fitch W. M. 1971; Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416 [CrossRef]
    [Google Scholar]
  13. Hall T. A. 1999; BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98
    [Google Scholar]
  14. Kim M. K., Im W.-T., Ohta H., Lee M., Lee S.-T. 2005; Sphingopyxis granuli sp. nov., a β -glucosidase-producing bacterium in the family Sphingomonadaceae in α -4 subclass of the Proteobacteria . J Microbiol 43:152–157
    [Google Scholar]
  15. Kimura M. 1983 The Neutral Theory of Molecular Evolution Cambridge: Cambridge University Press;
    [Google Scholar]
  16. Kumar S., Tamura K., Jakobsen I. B., Nei M. 2001; mega2: Molecular evolutionary genetics analysis software. Bioinformatics 17:1244–1245 [CrossRef]
    [Google Scholar]
  17. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [CrossRef]
    [Google Scholar]
  18. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  19. Sasser M. 1990; Identification of bacteria by gas chromatography of cellular fatty acids . MIDI Technical Note 101: Newark, DE: MIDI Inc;
    [Google Scholar]
  20. Schenkel E., Berlaimont V., Dubois J., Helson-Cambier M., Hanocq M. 1995; Improved high-performance liquid chromatographic method for the determination of polyamines as their benzoylated derivatives: application to P388 cancer cells. J Chromatogr B Biomed Appl 668:189–197 [CrossRef]
    [Google Scholar]
  21. Shin Y. K., Lee J.-S., Chun C. O., Kim H.-J., Park Y.-H. 1996; Isoprenoid quinone profiles of the Leclercia adecarboxylata KCTC 1036T . J Microbiol Biotechnol 6:68–69
    [Google Scholar]
  22. Stolz A., Bürger S., Kuhm A., Kämpfer P., Busse H.-J. 2005; Pusillimonas noertemannii gen. nov., sp. nov., a new member of the family Alcaligenaceae that degrades substituted salicylates. Int J Syst Evol Microbiol 55:1077–1081 [CrossRef]
    [Google Scholar]
  23. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [CrossRef]
    [Google Scholar]
  24. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [CrossRef]
    [Google Scholar]
  25. Vancanneyt M., Vandamme P., Kersters K. 1995; Differentiation of Bordetella pertussis , B. parapertussis , and B. bronchiseptica by whole-cell protein electrophoresis and fatty acid analysis. Int J Syst Bacteriol 45:843–847 [CrossRef]
    [Google Scholar]
  26. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
  27. Weisburg W. G., Barns S. M., Pelletier D. A., Lane D. J. 1991; 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 173:697–703
    [Google Scholar]
  28. Yabuuchi E., Yano I. 1981; Achromobacter gen. nov. and Achromobacter xylosoxidans (ex Yabuuchi and Ohyama 1971) nom. rev.. Int J Syst Bacteriol 31:477–478 [CrossRef]
    [Google Scholar]
/content/journal/ijsem/10.1099/ijs.0.009720-0
Loading
/content/journal/ijsem/10.1099/ijs.0.009720-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error