1887

Abstract

A novel betaproteobacterium, designated strain Ch07, was isolated from granules from the wastewater-treatment bioreactor of an alcohol fermentation factory in South Korea. In order to determine its taxonomic position, the novel strain was characterized using a polyphasic approach. The new strain was Gram-negative, facultatively anaerobic, non-spore-forming, motile and short rod-shaped. 16S rRNA gene sequence analysis revealed that strain Ch07 belonged to the class , being related to BN9 (gene sequence similarity 97.30 %), subsp. DSM 10346 (97.09 %), DSM 5571 (97.01 %), DSM 13608 (96.68 %) and DSM 1214 (96.47 %). The results of DNA–DNA hybridization tests showed that reassociation values were less than 62 % with respect to these closely related type strains. Chemotaxonomic data showed that strain Ch07 possessed ubiquinone Q-8. The G+C content of the genomic DNA was 67.9±0.1 mol%. The major polyamine of strain Ch07 was putrescine. The major polar lipids of strain Ch07 were phosphatidylethanolamine, followed by diphosphatidylglycerol and phosphatidylglycerol. When strain Ch07 was incubated on tryptic soy agar, the major cellular fatty acids were C, C cyclo, summed feature 3 (C 7/iso C 2-OH) and summed feature 5 (C 7/9/12). The results of DNA–DNA hybridizations, in combination with the chemotaxonomic and physiological data, demonstrated that strain Ch07 represents a novel species of a new genus, for which the name gen. nov., sp. nov. is proposed. The type strain of the type species is Ch07 (=KCTC 12668=LMG 24012).

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2010-06-01
2019-10-14
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vol. , part 6, pp. 1401 - 1406

Demonstration of fimbriae production by strain sp. nov. Ch07 .

Electron micrographs of cells of sp. nov. Ch07 and closely related type strains.

Two dimensional thin layer chromatogram of the polar lipids of sp. nov. Ch07 and BN9 .

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