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Abstract
A novel betaproteobacterium, designated strain Ch07T, was isolated from granules from the wastewater-treatment bioreactor of an alcohol fermentation factory in South Korea. In order to determine its taxonomic position, the novel strain was characterized using a polyphasic approach. The new strain was Gram-negative, facultatively anaerobic, non-spore-forming, motile and short rod-shaped. 16S rRNA gene sequence analysis revealed that strain Ch07T belonged to the class Betaproteobacteria, being related to Pusillimonas noertemannii BN9T (gene sequence similarity 97.30 %), Achromobacter xylosoxidans subsp. xylosoxidans DSM 10346T (97.09 %), Bordetella pertussis DSM 5571T (97.01 %), Pigmentiphaga kullae DSM 13608T (96.68 %) and Castellaniella defragrans DSM 1214T (96.47 %). The results of DNA–DNA hybridization tests showed that reassociation values were less than 62 % with respect to these closely related type strains. Chemotaxonomic data showed that strain Ch07T possessed ubiquinone Q-8. The G+C content of the genomic DNA was 67.9±0.1 mol%. The major polyamine of strain Ch07T was putrescine. The major polar lipids of strain Ch07T were phosphatidylethanolamine, followed by diphosphatidylglycerol and phosphatidylglycerol. When strain Ch07T was incubated on tryptic soy agar, the major cellular fatty acids were C16 : 0, C17 : 0 cyclo, summed feature 3 (C16 : 1 ω7c/iso C15 : 0 2-OH) and summed feature 5 (C18 : 1 ω7c/ω9t/ω12t). The results of DNA–DNA hybridizations, in combination with the chemotaxonomic and physiological data, demonstrated that strain Ch07T represents a novel species of a new genus, for which the name Parapusillimonas granuli gen. nov., sp. nov. is proposed. The type strain of the type species is Ch07T (=KCTC 12668T=LMG 24012T).
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