1887

Abstract

A Gram-negative, rod-shaped, yellow-pigmented bacterium, designated strain R2A-7, was isolated from sediment of the eutrophic Taihu Lake in Jiangsu Province, China. The isolate was subjected to a taxonomic study using a polyphasic approach. Phylogenetic analysis based on the 16S rRNA gene sequences placed strain R2A-7 within the genus in the family . The highest sequence similarity was found with (98.3 %), followed by other species with similarities <96.0 %. The major fatty acids (>5 %) were 15 : 0 iso, 17 : 1 iso 9, 17 : 0 iso 3-OH, 15 : 1 iso G and 15 : 0 iso 3-OH. The G+C content of the genomic DNA of strain R2A-7 was 37.7 mol%. The DNA–DNA relatedness value with CGMCC 1.3801 was 40.6 %. Molecular and phenotypic data suggest that strain R2A-7 represents a novel species within the genus , for which the name is proposed. The type strain is R2A-7 (=CGMCC 1.7270=NBRC 104929).

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2009-11-01
2024-04-16
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References

  1. Bergey, D. H., Harrison, F. C., Breed, R. S., Hammer, B. W. & Huntoon, F. M. (editors)(1923).Bergey's Manual of Determinative Bacteriology. Baltimore: Williams & Wilkins.
  2. Bernardet, J. F. & Bowman, J.(2006). The genus Flavobacterium. In The Prokaryotes, a Handbook on the Biology of Bacteria, 3rd edn, vol. 7, pp. 481–531. Edited by M. Dworkin, S. Falkow, E. Rosenberg, K. H. Schleifer & E. Stackebrandt. New York: Springer.
  3. Bernardet, J. F., Segers, P., Vancanneyt, M., Berthe, F., Kersters, K. & Vandamme, P.(1996). Cutting a Gordian knot: emended classification and description of the genus Flavobacterium, emended description of the family Flavobacteriaceae, and proposal of Flavobacterium hydatis nom. nov. (basonym, Cytophaga aquatilis Strohl and Tait 1978). Int J Syst Bacteriol 46, 128–148.[CrossRef] [Google Scholar]
  4. Bernardet, J. F., Nakagawa, Y. & Holmes, B.(2002). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52, 1049–1070.[CrossRef] [Google Scholar]
  5. Cao, S.-J., Qu, J.-H., Yang, J.-S., Sun, Q. & Yuan, H.-L.(2008).Halolactibacillus alkaliphilus sp. nov., a moderately alkaliphilic and halophilic bacterium isolated from a soda lake in Inner Mongolia, China, and emended description of the genus Halolactibacillus. Int J Syst Evol Microbiol 58, 2169–2173.[CrossRef] [Google Scholar]
  6. Chun, J., Lee, J.-H., Jung, Y., Kim, M., Kim, S., Kim, B. K. & Lim, Y. W.(2007). EzTaxon: A web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequence. Int J Syst Evol Microbiol 57, 2259–2261.[CrossRef] [Google Scholar]
  7. De Ley, J.(1970). Reexamination of the association between melting point, buoyant density, and chemical base composition of deoxyribonucleic acid. J Bacteriol 101, 738–754. [Google Scholar]
  8. Dong, X.-Z. & Cai, M.-Y.(2001).Determinative Manual for Routine Bacteriology. Beijing: Scientific Press.
  9. Guo, L.(2007). Ecology. Doing Battle with the green monster of Taihu Lake. Science 317, 1166[CrossRef] [Google Scholar]
  10. Kim, B.-Y., Weon, H.-Y., Cousin, S., Yoo, S.-H., Kwon, S.-W., Go, S.-J. & Stackebrandt, E.(2006).Flavobacterium daejeonense sp. nov. and Flavobacterium suncheonense sp. nov., isolated from greenhouse soil in Korea. Int J Syst Evol Microbiol 56, 1645–1649.[CrossRef] [Google Scholar]
  11. Kumar, S., Tamura, K. & Nei, M.(2004). MEGA3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef] [Google Scholar]
  12. Marmur, J.(1961). A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3, 208–218.[CrossRef] [Google Scholar]
  13. Sambrook, J. & Russell, D. W.(2001).Molecular Cloning, a Laboratory Manual, 3rd edn. NY: Cold Spring Harbor Laboratory.
  14. Thompson, J. D., Higgins, D. G. & Gibson, T. J.(1994).clustalw: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22, 4673–4680.[CrossRef] [Google Scholar]
  15. Wang, Z.-W., Liu, Y.-H., Dai, X., Wang, B.-J., Jiang, C.-Y. & Liu, S.-J.(2006).Flavobacterium saliperosum sp. nov., isolated from freshwater lake sediment. Int J Syst Evol Microbiol 56, 439–442.[CrossRef] [Google Scholar]
  16. Weon, H.-Y., Song, M.-H., Son, J.-A., Kim, B.-Y., Kwon, S. W., Go, S.-J. & Stackebrandt, E.(2007).Flavobacterium terrae sp. nov. and Flavobacterium cucumis sp. nov., isolated from greenhouse soil. Int J Syst Evol Microbiol 57, 1594–1598.[CrossRef] [Google Scholar]
  17. Yi, H. & Chun, J.(2006).Flavobacterium weaverense sp. nov. and Flavobacterium segetis sp. nov., novel psychrophiles isolated from the Antarctic. Int J Syst Evol Microbiol 56, 1239–1244.[CrossRef] [Google Scholar]
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vol. , part 11, pp. 2666 - 2669

Maximum-parsimony and minimum-evolution phylogenetic trees based on 16S rRNA gene sequences. [PDF](19 KB)



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