An actinomycete, strain DMKUA 245, isolated from soil, was investigated using a polyphasic approach. The isolate formed longitudinally paired spores on the tips of short sporophores that branched alternately from aerial hyphae. The morphological and chemotaxonomic properties clearly demonstrated that the new isolate belonged to the genus . 16S rRNA gene sequence analysis supported the assignment of the novel strain to the genus . The gene sequence similarity values between the novel strain and the closely related species , subsp. , subsp. and were 98.4 %, 97.4 %, 97.0 % and 96.9 %, respectively. The DNA–DNA hybridization values and some physiological and biochemical properties indicated that strain DMKUA 245 could be distinguished from its phylogenetically closest relatives. Based on these genotypic and phenotypic data, strain DMKUA 245 represents a novel species in the genus for which the name sp. nov. is proposed. The type strain is strain DMKUA 245 (=BCC 14407=NBRC 104113). In addition, DNA–DNA relatedness values in reciprocal hybridization experiments showed that was a separate genomic species from subsp. . A combination of genotypic and phenotypic data supported the classification of as a separate species.


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