1887

Abstract

A novel obligately organotrophic, facultatively microaerophilic spirillum, designated strain D-427, was isolated from sulfidic sludge of a municipal wastewater-treatment plant. Cells were Gram-negative, large and highly motile due to bipolar tufts of flagella covered with mucous sheaths. Coccoid cells were sometimes formed. Strain D-427 grew optimally at pH 7.5–7.8 and 28 °C in the presence of 2 % O in the gas phase. The organism showed oxidase and very low catalase activity. The isolate grew chemo-organotrophically with a limited number of organic acids as substrates. The DNA G+C content was 38.0 mol% ( ). Phylogenetic analysis of the 16S rRNA gene sequence placed strain D-427 in the genus within the class . The 16S rRNA gene sequence similarity between strain D-427 and ATCC 19554, the type strain of the single species of the genus, was 98.6 %. The low level of DNA–DNA hybridization and different phenotypic properties indicate that strain D-427 is clearly distinguishable from . No strain of is available from any established culture collection or from the authors who described this species. Therefore, on the basis of phenotypic and genotypic data and the fact that the type and single species of the genus cannot be included in any scientific study, since the type strain has been lost, we propose to assign strain D-427 as a novel species of the genus , sp. nov. (type strain D-427 =DSM 12756 =VKM B-2518), and we request that the Judicial Commission place the name on the list of rejected names if a suitable type strain is not found or a neotype is not proposed within 2 years following the publication of this paper. An emended description of the genus is also provided.

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2009-11-01
2019-08-18
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References

  1. Caraway, B. H. & Krieg, N. R. ( 1974; ). Aerotaxis in Spirillum volutans. Can J Microbiol 20, 1367–1377.[CrossRef]
    [Google Scholar]
  2. Cole, J. A. & Rittenberg, S. C. ( 1971; ). A comparison of respiratory processes in Spirillum volutans, Spirillum itersonii and Spirillum serpens. J Gen Microbiol 69, 375–383.[CrossRef]
    [Google Scholar]
  3. De Ley, J., Cattoir, H. & Reynaerts, A. ( 1970; ). The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12, 133–142.[CrossRef]
    [Google Scholar]
  4. Dubinina, G. A., Grabovich, M. Y., Churikova, V. V., Lysenko, A. M. & Chernych, N. A. ( 1993; ). Reevaluation of the taxonomic status of the colorless sulphur spirilla belonging to the genus Thiospira and description of new species Aquaspirillum bipunctata comb. nov. Microbiology (English translation of Mikrobiologiia) 62, 1101–1112.
    [Google Scholar]
  5. Ehrenberg, C. G. ( 1832; ). Beiträge zur Kenntnis der Organization der Infusorien und ihrer geographischen Verbreitung besonders in Sibirien. Abh K Akad Wiss Berlin 1830, 1–88 (in German).
    [Google Scholar]
  6. Fitch, W. M. ( 1971; ). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20, 406–416.[CrossRef]
    [Google Scholar]
  7. Hylemon, P. B., Wells, J. S., Jr, Bowdre, J. H., MacAdoo, T. O. & Krieg, N. R. ( 1973a; ). Designation of Spirillum volutans Ehrenberg 1832 as type species of the genus Spirillum Ehrenberg 1832 and designation of the neotype strain of S. volutans. Request for an Opinion. Int J Syst Bacteriol 23, 20–27.[CrossRef]
    [Google Scholar]
  8. Hylemon, P. B., Wells, J. S., Jr, Krieg, N. R. & Jannasch, H. W. ( 1973b; ). The genus Spirillum: a taxonomic study. Int J Syst Bacteriol 23, 340–380.[CrossRef]
    [Google Scholar]
  9. Jukes, T. H. & Cantor, C. R. ( 1969; ). Evolution of protein molecules. In Mammalian Protein Metabolism, vol. 3, pp. 21–132. Edited by H. N. Munro. New York: Academic Press.
  10. Krieg, N. R. ( 2005; ). Genus I. Spirillum Ehrenberg 1832, 38AL. In Bergey's Manual of Systematic Bacteriology, 2nd edn, vol. 2, part C, pp. 870–880. Edited by D. J. Brenner, N. R. Krieg, J. T. Staley & G. M. Garrity. New York: Springer.
  11. Kucera, S. & Wolfe, R. S. ( 1957; ). A selective enrichment method for Gallionella ferrugineae. J Bacteriol 74, 344–349.
    [Google Scholar]
  12. Kuenen, J. G. & Dubinina, G. A. ( 2005; ). Genus VIII. Thiospira Visloukh 1914, 48AL. In Bergey's Manual of Systematic Bacteriology, 2nd edn, vol. 2, part B, pp. 178–179. Edited by D. J. Brenner, N. R. Krieg, J. T. Staley & G. M. Garrity. New York: Springer.
  13. Lane, D. J. ( 1991; ). 16S/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics, pp. 115–175. Edited by E. Stackebrandt & M. Goodfellow. Chichester: Wiley.
  14. Lapage, S. P., Sneath, P. H. A., Lessel, F. F., Skerman, V. B. D., Seeliger, H. P. R. & Clark, W. A. (editors) ( 1992; ). International Code of Nomenclature of Bacteria (1990 Revision). Bacteriological Code. Washington, DC: American Society for Microbiology.
  15. Luft, J. U. ( 1971; ). Ruthenium red and violet. II. Fine structure localization in animal tissues. Anat Res 171, 369–415.[CrossRef]
    [Google Scholar]
  16. Marmur, J. A. ( 1961; ). A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3, 208–218.[CrossRef]
    [Google Scholar]
  17. Medlin, L., Elwood, H. J., Stickel, S. & Sogin, M. L. ( 1988; ). The characterization of enzymatically amplified eukaryotic 16S-like rRNA-coding regions. Gene 71, 491–499.[CrossRef]
    [Google Scholar]
  18. Owen, R. J. & Lapage, S. P. ( 1976; ). The thermal denaturation of partly purified bacterial deoxyribonucleic acid and its taxonomic applications. J Appl Bacteriol 41, 335–340.[CrossRef]
    [Google Scholar]
  19. Pfennig, N. D. & Lippert, K. D. ( 1966; ). Über das Vitamin B12-Bedürfnis phototropher Schwefelbakterien. Arch Mikrobiol 55, 245–256 (in German).[CrossRef]
    [Google Scholar]
  20. Podkopaeva, D. A., Grabovich, M. Yu. & Dubinina, G. A. ( 2005; ). The functional role of reduced inorganic sulfur compounds in the metabolism of the microaerophilic bacterium Spirillum winogradskii. Microbiology (English translation of Mikrobiologiia) 74, 12–19.
    [Google Scholar]
  21. Podkopaeva, D. A., Grabovich, M. Yu., Dubinina, G. A., Lysenko, A. M., Tourova, T. P. & Kolganova, T. V. ( 2006; ). Two new species of microaerophilic sulfur spirilla, Spirillum winogradskii sp. nov. and Spirillum kriegii sp. nov. Microbiology (English translation of Mikrobiologiia) 75, 172–179.
    [Google Scholar]
  22. Rittenberg, B. T. & Rittenberg, S. C. ( 1962; ). The growth of Spirillum volutans in mixed and pure cultures. Arch Mikrobiol 42, 138–153.[CrossRef]
    [Google Scholar]
  23. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  24. Stead, D. E., Sellwood, J. E., Wilson, J. & Viney, I. ( 1992; ). Evaluation of a commercial microbial identification system based on fatty acid profiles for rapid, accurate identification of plant pathogenic bacteria. J Appl Bacteriol 72, 315–321.[CrossRef]
    [Google Scholar]
  25. Swofford, D. L. ( 1998; ). paup*. Phylogenetic analysis using parsimony (* and other methods), version 4. Sunderland, MA: Sinauer Associates.
  26. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  27. Van de Peer, Y. & De Wachter, R. ( 1994; ). treecon for Windows: a software package for the construction and drawing of evolutionary trees for the Microsoft Windows environment. Comput Appl Biosci 10, 569–570.
    [Google Scholar]
  28. Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A. D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray, R. G. E. & other authors ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef]
    [Google Scholar]
  29. Wells, J. S., Jr & Krieg, N. R. ( 1965; ). Cultivation of Spirillum volutans in a bacteria-free environment. J Bacteriol 90, 817–818.
    [Google Scholar]
  30. Williams, M. A. & Rittenberg, S. C. ( 1957; ). A taxonomic study of the genus Spirillum Ehrenberg. Int Bull Bacteriol Nomencl Taxon 7, 49–110.
    [Google Scholar]
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vol. , part 11, pp. 2916 - 2920

(a, b) Phase-contrast micrographs showing morphology of strain D-427 with coccoid bodies in the stationary growth phase (a) and the accumulation of elemental sulfur in cells grown on MPSS medium with FeS (b). Bar, 7 µm. (c, d) Transmission electron micrographs of strain D-427 showing sheathed flagella arranged in tufts (c) (whole-cell preparation stained with 1 % ammonium molybdate; bar, 1 µm) and the ultrastructure, with capsules of a polysaccharide nature stained with ruthenium red (d) (bar, 4 µm).

Cellular fatty acid composition of strain D-427

Values are percentages of total fatty acids. a, Anteiso-branched; i, iso-branched.



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