1887

Abstract

A Gram-positive, aerobic or facultatively anaerobic, rod-shaped, spore-forming bacterium, strain Gsoil 1138, was isolated from soil of a ginseng field in Pocheon Province, South Korea, and was characterized in order to determine its taxonomic position. On the basis of 16S rRNA gene sequence analysis, strain Gsoil 1138 was shown to belong to the family and was most closely related to the type strains of (98.2 % similarity) and (96.5 %). Levels of 16S rRNA gene sequence similarity between strain Gsoil 1138 and the type strains of other recognized species of the genus were below 96.5 %. The G+C content of the genomic DNA of strain Gsoil 1138 was 52.1±0.2 mol% (mean± of three determinations). Phenotypic and chemotaxonomic data (MK-7 as the major menaquinone and anteiso-C and iso-C as the predominant fatty acids) supported the affiliation of strain Gsoil 1138 to the genus . The results of DNA–DNA hybridization experiments and physiological and biochemical tests allowed strain Gsoil 1138 to be distinguished genotypically and phenotypically from recognized species of the genus . Strain Gsoil 1138 is therefore considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is Gsoil 1138 (=KCTC 13941=LMG 23404).

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2010-05-01
2019-10-18
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Negatively stained transmission electron micrograph of a single cell of strain Gsoil 1138 showing peritrichous flagella. Bar, 2µm.

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Phylogenetic tree constructed from a comparative analysis of 16S rRNA gene sequences showing the relationships of sp. nov. Gsoil 1138 with other species of the genus . [ PDF] 63 KB

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Cellular fatty acid profile of sp. nov. Gsoil 1138 and related type strains of species of genus . [ PDF] 28 KB

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