1887

Abstract

Three pathogenic variants (i.e. pathotypes) have been described within pv. , the causal agent of Asiatic citrus canker. Pathotype A strains naturally infect a wide range of species and members of some related genera. In contrast, pathotypes A* and A have narrow host ranges within the genus and have been isolated from Mexican lime ( L.) and from Mexican lime and alemow ( L.), respectively. We used amplified fragment length polymorphism (AFLP) and multilocus sequence analysis (MLSA) based on four partial housekeeping gene sequences (, , and ) for the genotypic classification of pv. and the poorly characterized citrus pathogen pv. . A Mantel test showed that genetic distances derived from AFLP and MLSA were highly correlated. pv. showed a close relatedness to the type strain of , indicating that this pathovar should be reclassified as pv. . All pathotype A* and A strains were most closely related to pv. strains with a wide host range (pathotype A), confirming previous DNA–DNA hybridization data. Pathotype A should be considered a junior synonym of pathotype A* on the basis of pathogenicity tests, AFLP, MLSA and PCR using pathovar-specific primers. Evolutionary genome divergences computed from AFLP data suggested that pathotype A* (including A strains) is a group of strains that shows a wider genetic diversity than pathotype A.

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2010-03-01
2024-03-28
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