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Abstract

A novel, strictly anaerobic, thermophilic, sulfate-reducing bacterium, designated strain AT1325, was isolated from a deep-sea hydrothermal vent at the Rainbow site on the Mid-Atlantic Ridge. This strain was subjected to a polyphasic taxonomic analysis. Cells were Gram-negative motile rods (approximately 2.4×0.6 μm) with a single polar flagellum. Strain AT1325 grew at 55–75 °C (optimum, 65–70 °C), at pH 5.5–8.0 (optimum, 6.5–7.5) and in the presence of 1.5–4.5 % (w/v) NaCl (optimum, 2.5 %). Cells grew chemolithoautotrophically with H as an energy source and as an electron acceptor. Alternatively, the novel isolate was able to use methylamine, peptone or yeast extract as carbon sources. The dominant fatty acids (>5 % of the total) were C, C 7, C and C cyclo 8. The G+C content of the genomic DNA of strain AT1325 was 45.6 mol%. Phylogenetic analyses based on 16S rRNA gene sequences placed strain AT1325 within the family , in the bacterial domain. Comparative 16S rRNA gene sequence analysis indicated that strain AT1325 belonged to the genus , sharing 97.8 % similarity with the type strain of the unique representative species of this genus. On the basis of the data presented, it is suggested that strain AT1325 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is AT1325 (=DSM 21156=JCM 15391).

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2010-01-01
2019-12-09
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References

  1. Alain, K., Querellou, J., Lesongeur, F., Pignet, P., Crassous, P., Raguénès, G., Cueff, V. & Cambon-Bonavita, M.-A. ( 2002; ). Caminibacter hydrogeniphilus gen. nov., sp. nov., a novel thermophilic, hydrogen-oxidizing bacterium isolated from an East Pacific Rise hydrothermal vent. Int J Syst Evol Microbiol 52, 1317–1323.[CrossRef]
    [Google Scholar]
  2. Altschul, S. F., Gish, W., Miller, W., Myers, E. & Lipman, D. ( 1990; ). Basic local alignment search tool. J Mol Biol 215, 403–410.[CrossRef]
    [Google Scholar]
  3. Cord-Ruwisch, R. ( 1985; ). A quick method for the determination of dissolved and precipitated sulfides in cultures of sulfate-reducing bacteria. J Microbiol Methods 4, 33–36.[CrossRef]
    [Google Scholar]
  4. Felsenstein, J. ( 1981; ). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17, 368–376.[CrossRef]
    [Google Scholar]
  5. Felsenstein, J. ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  6. Galtier, N., Gouy, M. & Gautier, C. ( 1996; ). seaview and phylo_win: two graphic tools for sequence alignment and molecular phylogeny. Comput Appl Biosci 12, 543–548.
    [Google Scholar]
  7. Garrity, G. M. & Holt, J. G. ( 2001; ). Phylum BIII. Thermodesulfobacteria phy. nov. In Bergey's Manual of Systematic Bacteriology, 2nd edn, vol. 1, pp. 389–393. Edited by D. R. Boone, R. W. Castenholz & G. M. Garrity. New York: Springer.
  8. Hatchikian, E. C., Ollivier, B. & Garcia, J.-J. ( 2001; ). Family I. Thermodesulfobacteriaceae fam. nov. In Bergey's Manual of Systematic Bacteriology, 2nd edn, vol. 1, p. 390. Edited by D. R. Boone, R. W. Castenholz & G. M. Garrity. New York: Springer.
  9. Itoh, T., Suzuki, K.-I., Sanchez, P. C. & Nakase, T. ( 1999; ). Caldivirga maquilingensis gen. nov., sp. nov., a new genus of rod-shaped crenarchaeote isolated from a hot spring in the Philippines. Int J Syst Bacteriol 49, 1157–1163.[CrossRef]
    [Google Scholar]
  10. Jeanthon, C., L'Haridon, S., Cueff, V., Banta, A., Reysenbach, A.-L. & Prieur, D. ( 2002; ). Thermodesulfobacterium hydrogeniphilum sp. nov., a thermophilic, chemolithoautotrophic, sulfate-reducing bacterium isolated from a deep-sea hydrothermal vent at Guaymas Basin, and emendation of the genus Thermodesulfobacterium. Int J Syst Evol Microbiol 52, 765–772.[CrossRef]
    [Google Scholar]
  11. Kämpfer, P. & Kroppenstedt, R. M. ( 1996; ). Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 42, 989–1005.[CrossRef]
    [Google Scholar]
  12. Kashefi, K., Holmes, D. E., Reysenbach, A.-L. & Lovley, D. R. ( 2002; ). Use of Fe(III) as an electron acceptor to recover previously uncultured hyperthermophiles: isolation and characterization of Geothermobacterium ferrireducens gen. nov. Appl Environ Microbiol 68, 1735–1742.[CrossRef]
    [Google Scholar]
  13. Marmur, J. & Doty, P. ( 1962; ). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5, 109–118.[CrossRef]
    [Google Scholar]
  14. Moussard, H., L'Haridon, S., Tindall, B. J., Banta, A., Schumann, P., Stackebrandt, E., Reysenbach, A.-L. & Jeanthon, C. ( 2004; ). Thermodesulfatator indicus gen. nov., sp. nov., a novel thermophilic chemolithoautotrophic sulfate-reducing bacterium isolated from the Central Indian Ridge. Int J Syst Evol Microbiol 54, 227–233.[CrossRef]
    [Google Scholar]
  15. Nakagawa, T. & Fukui, M. ( 2003; ). Molecular characterization of community structures and sulfur metabolism within microbial streamers in Japanese hot springs. Appl Environ Microbiol 69, 7044–7057.[CrossRef]
    [Google Scholar]
  16. Nakagawa, S., Takai, K., Inagaki, F., Chiba, H., Ishibashi, J., Kataoka, S., Hirayama, H., Nunoura, T., Horikoshi, K. & Sako, Y. ( 2005; ). Variability in microbial community and venting chemistry in a sediment-hosted backarc hydrothermal system: impacts of subseafloor phase-separation. FEMS Microbiol Ecol 54, 141–155.[CrossRef]
    [Google Scholar]
  17. Postec, A., Urios, L., Lesongeur, F., Ollivier, B., Querellou, J. & Godfroy, A. ( 2005; ). Continuous enrichment culture and molecular monitoring to investigate the microbial diversity of thermophiles inhabiting deep-sea hydrothermal ecosystems. Curr Microbiol 50, 138–144.[CrossRef]
    [Google Scholar]
  18. Postec, A., Lesongeur, F., Pignet, P., Ollivier, B., Querellou, J. & Godfroy, A. ( 2007; ). Continuous enrichment cultures: insights into prokaryotic diversity and metabolic interactions in deep-sea vent chimneys. Extremophiles 11, 747–757.[CrossRef]
    [Google Scholar]
  19. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  20. Skirnisdottir, S., Hreggvidsson, G. O., Hjörleifsdottir, S., Marteinsson, V. T., Petursdottir, S. K., Holst, O. & Kristjansson, J. K. ( 2000; ). Influence of sulfide and temperature on species composition and community structure of hot spring microbial mats. Appl Environ Microbiol 66, 2835–2841.[CrossRef]
    [Google Scholar]
  21. Stackebrandt, E. & Ebers, J. ( 2006; ). Taxonomic parameters revisited: tarnished gold standards. Microbiol Today 33, 152–155.
    [Google Scholar]
  22. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  23. Widdel, F. & Bak, F. ( 1992; ). Gram-negative mesophilic sulfate-reducing bacteria. In The Prokaryotes, pp. 3352–3378. Edited by A. Balows, H. G. Trüper, M. Dworkin, W. Harder & K.-H Schleifer. New York: Springer.
  24. Zeikus, J. G., Dawson, M.-A., Thompson, T. E., Ingvorsen, K. & Hatchikian, E. C. ( 1983; ). Microbial ecology of volcanic sulphidogenesis: isolation and characterization of Thermodesulfobacterium commune gen. nov. and sp. nov. J Gen Microbiol 129, 1159–1169.
    [Google Scholar]
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Micrographs of cells of strain AT1325 ( sp. nov.). [PDF](162 KB)

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Graph showing the effects of temperature on the maximum growth rate of strain AT1325 . [PDF](15 KB)

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