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Abstract

Resequencing of the 16S rRNA gene of the type strain of Ding and Yokota 2004 revealed 99.9 % sequence similarity to that of the type strain of (Leifson 1962) Ding and Yokota 2004. This high phylogenetic relatedness of . and . was confirmed by the results of DNA–DNA hybridization between . DSM 10281 and . ATCC BAA-806 (reassociation value 96 %). Therefore, it is proposed to reclassify the type strain of . as a strain of . . However, the genome of the type strain of . is about 0.9 Mb larger than that of the . type strain. This results in a decrease in the reassociation value in the reciprocal DNA–DNA hybridization to 72 %, a level slightly above the threshold for delineating bacterial species. These data and distinctive phenotypic characteristics indicate that the name is a later heterotypic synonym of and permit the description of two novel subspecies, subsp. subsp. nov. (type strain ATCC 14670 =JCM 21423 =DSM 10281) and subsp. subsp. nov., comb. nov. (type strain 7-2 =JCM 21495 =ATCC BAA-806). Three bacterial strains, IEH 4430, IEH 4515 and IEH 8757, isolated from water were found to be the closest relatives of these strains. Strain IEH 8757 was classified as a strain of subsp. . Studies of genotypic and phenotypic features of strains IEH 4430 and IEH 4515 showed that the strains represent a novel species, which is most closely related to and for which the name sp. nov. is proposed (type strain IEH 4430 =DSM 21191 =ATCC BAA-1628).

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2010-06-01
2020-01-26
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vol. , part 6, pp. 1418 - 1426

Transmission electron microscopy image of a cell of strain IEH 4430 . Bar, 200 nm.



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