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Volume 59, Issue 10

gen. nov., sp. nov. and sp. nov., poly--hydroxybutyrate-producing bacteria in the family Free

Abstract

Two Gram-negatively stained, facultatively anaerobic, non-motile, vibrioid and rod-shaped, chemoheterotrophic bacterial strains, designated YIM D82 and YIM D812, were isolated from a salt mine in Yunnan, south-west China. DNA–DNA hybridization, genomic DNA G+C content and phylogenetic analyses based on 16S rRNA gene sequences divided the two isolates into two distinct genospecies that were also clearly differentiated by fatty acid profiles, carbon source utilization patterns, antibiotic susceptibility and biochemical characteristics. The two isolates grew in the presence of 1.5–20 % NaCl, and optimally at 28 °C and pH 7.5. The genomic DNA G+C contents of strains YIM D82 and YIM D812 were 61.5 and 62.3 mol%, respectively. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strains YIM D82 and YIM D812 were members of the family and showed 90.5–90.6 % and 90.1–90.2 % similarities with their closest relatives, and , respectively. Differential phenotypic and genotypic characteristics of the two isolates from recognized genera showed that the two strains should be classified as representing a new genus and two novel species for which the names gen. nov., sp. nov. (type strain YIM D82=DSM 21159=KCTC 22351) and sp. nov. (type strain YIM D812=CCTCC AA 208037=DSM 21160) are proposed.

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Keyword(s): PHB, poly-β-hydroxybutyrate
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2009-10-01
2024-04-24
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Fodinicurvata sediminis gen. nov., sp. nov. and Fodinicurvata fenggangensis sp. nov., poly-β-hydroxybutyrate-producing bacteria in the family Rhodospirillaceae
Int J Syst Evol Microbiol 59, 2575 (2009); https://doi.org/10.1099/ijs.0.009340-0
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Two-dimensional thin-layer chromatograms of the polar lipids of strains YIM D82 ( gen. nov., sp. nov.) and D812 ( sp. nov.). [PDF](24 KB)

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Cellular fatty acid contents of strains YIM D82 ( gen. nov., sp. nov.) and D812 ( sp. nov.). [PDF](40 KB) (

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