1887

Abstract

Two Gram-negatively stained, facultatively anaerobic, non-motile, vibrioid and rod-shaped, chemoheterotrophic bacterial strains, designated YIM D82 and YIM D812, were isolated from a salt mine in Yunnan, south-west China. DNA–DNA hybridization, genomic DNA G+C content and phylogenetic analyses based on 16S rRNA gene sequences divided the two isolates into two distinct genospecies that were also clearly differentiated by fatty acid profiles, carbon source utilization patterns, antibiotic susceptibility and biochemical characteristics. The two isolates grew in the presence of 1.5–20 % NaCl, and optimally at 28 °C and pH 7.5. The genomic DNA G+C contents of strains YIM D82 and YIM D812 were 61.5 and 62.3 mol%, respectively. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strains YIM D82 and YIM D812 were members of the family and showed 90.5–90.6 % and 90.1–90.2 % similarities with their closest relatives, and , respectively. Differential phenotypic and genotypic characteristics of the two isolates from recognized genera showed that the two strains should be classified as representing a new genus and two novel species for which the names gen. nov., sp. nov. (type strain YIM D82=DSM 21159=KCTC 22351) and sp. nov. (type strain YIM D812=CCTCC AA 208037=DSM 21160) are proposed.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.009340-0
2009-10-01
2020-01-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/59/10/2575.html?itemId=/content/journal/ijsem/10.1099/ijs.0.009340-0&mimeType=html&fmt=ahah

References

  1. Allgaier, M., Uphoff, H., Feelske, A. & Wagner-Döbler, I. ( 2003; ). Aerobic anoxygenic photosynthesis in Roseobacter clade bacteria from diverse marine habitats. Appl Environ Microbiol 69, 5051–5059.[CrossRef]
    [Google Scholar]
  2. Altschul, S. F., Gish, W., Miller, W., Myers, E. W. & Lipman, D. J. ( 1990; ). Basic local alignment search tool. J Mol Biol 215, 403–410.[CrossRef]
    [Google Scholar]
  3. Cho, J. C. & Giovannoni, S. J. ( 2006; ). Pelagibaca bermudensis gen. nov., sp. nov., a novel marine bacterium within the Roseobacter clade in the order Rhodobacterales. Int J Syst Evol Microbiol 56, 855–859.[CrossRef]
    [Google Scholar]
  4. Coenye, T., Goris, J., Spilker, T., Vandamme, P. & LiPuma, J. J. ( 2002; ). Characterization of unusual bacteria isolated from respiratory secretions of cystic fibrosis patients and description of Inquilinus limosus gen. nov., sp. nov. J Clin Microbiol 40, 2062–2069.[CrossRef]
    [Google Scholar]
  5. Cohen-Bazire, G., Sistrom, W. R. & Stanier, R. Y. ( 1957; ). Kinetic studies of pigment synthesis by nonsulfur purple bacteria. J Cell Comp Physiol 49, 25–68.[CrossRef]
    [Google Scholar]
  6. Collins, M. D. & Jones, D. ( 1980; ). Lipids in the classification and identification of coryneform bacteria containing peptidoglycans based on 2,4-diaminobutyric acid. J Appl Bacteriol 48, 459–470.[CrossRef]
    [Google Scholar]
  7. Collins, M. D., Pirouz, T., Goodfellow, M. & Minnikin, D. E. ( 1977; ). Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100, 221–230.[CrossRef]
    [Google Scholar]
  8. Cui, X.-L., Mao, P.-H., Zeng, M., Li, W.-J., Zhang, L.-P., Xu, L.-H. & Jiang, C.-L. ( 2001; ). Streptomonospora salina gen. nov., sp. nov., a new member of the family Nocardiopsaceae. Int J Syst Evol Microbiol 51, 357–363.
    [Google Scholar]
  9. Eckert, B., Weber, O. B., Kirchhof, G., Halbritter, A., Stoffels, M. & Hartmann, A. ( 2001; ). Azospirillum doebereinerae sp. nov., a nitrogen-fixing bacterium associated with the C4-grass Miscanthus. Int J Syst Evol Microbiol 51, 17–26.
    [Google Scholar]
  10. Ezaki, T., Hashimoto, Y. & Yabuuchi, E. ( 1989; ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39, 224–229.[CrossRef]
    [Google Scholar]
  11. Favinger, J., Stadtwald, R. & Howard, G. ( 1989; ). Rhodospirillum centenum, sp. nov., a thermotolerant cyst-forming anoxygenic photosynthetic bacterium. Antonie Van Leeuwenhoek 55, 291–296.[CrossRef]
    [Google Scholar]
  12. Felsenstein, J. ( 1981; ). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17, 368–376.[CrossRef]
    [Google Scholar]
  13. Felsenstein, J. ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  14. Felsenstein, J. ( 1993; ). phylip (phylogeny inference package), version 3.5c. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle, USA.
  15. Garrity, G. M., Bell, J. A. & Lilburn, T. ( 2005; ). Family I. Rhodospirillaceae Pfennig and Trüper 1971, 17AL. In Bergey's Manual of Systematic Bacteriology, 2nd edn, vol. 2, The Proteobacteria, part C, The Alpha-, Beta-, Delta-, and Epsilonprotebacteria, pp. 1–40. Edited by D. J. Brenner, N. R. Krieg, J. T. Staley & G. M. Garrity. New York: Springer.
  16. Gregersen, T. ( 1978; ). Rapid method for distinction of Gram-negative from Gram-positive bacteria. Eur J Appl Microbiol Biotechnol 5, 123–127.[CrossRef]
    [Google Scholar]
  17. Imhoff, J. F., Petri, R. & Suling, J. ( 1998; ). Reclassification of species of the spiral-shaped phototrophic purple non-sulfur bacteria of the a-Proteobacteria: description of the new genera Phaeospirillum gen. nov., Rhodovibrio gen. nov., Rhodothalassium gen. nov. and Roseospira gen. nov. as well as transfer of Rhodospirillum fulvum to Phaeospirillum fulvum comb. nov., of Rhodospirilllum molischianum to Phaeospirillum molischianum comb. nov., of Rhodospirilium salinarum to Rhodovibrio salinarum comb. nov., of Rhodospirillum sodomense to Rhodovibrio sodomensis comb. nov., of Rhodospirillum salexigens to Rhodothalassium salexigens comb. nov. and of Rhodospirillum mediosalinum to Roseospira mediosalina comb. nov. Int J Syst Bacteriol 48, 793–798.[CrossRef]
    [Google Scholar]
  18. Kawasaki, H., Hoshino, Y., Kuraiski, Y. & Yamasato, K. ( 1992; ). Rhodocista centenaria gen. nov., sp. nov., a cyst-forming anoxygenic photosynthetic bacterium and its phylogenetic position in the Proteobacteria alpha group. J Gen Appl Microbiol 38, 541–551.[CrossRef]
    [Google Scholar]
  19. Khammas, K. M., Ageron, E., Grimont, P. A. D. & Kaiser, P. ( 1989; ). Azospirillum irakense sp. nov., a nitrogen-fixing bacterium associated with rice roots and rhizosphere soil. Res Microbiol 140, 679–693.
    [Google Scholar]
  20. Kimura, M. ( 1980; ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16, 111–120.[CrossRef]
    [Google Scholar]
  21. Kovacs, N. ( 1956; ). Identification of Pseudomonas pyocyanea by oxidase reaction. Nature 178, 703–704.
    [Google Scholar]
  22. Kumar, S., Tamura, K. & Nei, M. ( 2004; ). mega3: integrated software for Molecular Evolutionary Genetics Analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef]
    [Google Scholar]
  23. Labrenz, M., Tindall, B. J., Lawson, P. A., Collins, M. D., Schumann, P. & Hirsch, P. ( 2000; ). Staleya guttiformis gen. nov., sp. nov. and Sulfitobacter brevis sp. nov., α-3-Proteobacteria from hypersaline, heliothermal and meromictic antarctic Ekho Lake. Int J Syst Evol Microbiol 50, 303–313.[CrossRef]
    [Google Scholar]
  24. Mack, E. E., Mandelco, L., Woese, C. R. & Madigan, M. T. ( 1993; ). Rhodospirillum sodomense, sp. nov., a Dead Sea Rhodospirillum species. Arch Microbiol 160, 363–371.
    [Google Scholar]
  25. Magee, C. M., Rodeheaver, G. & Edgerton, R. F. ( 1975; ). A more reliable gram staining technique for diagnosis of surgical infections. Am J Surg 130, 341–346.[CrossRef]
    [Google Scholar]
  26. Maszenan, A. M., Seviour, R. J., Patel, B. K. C., Janssen, P. H. & Wanner, J. ( 2005; ). Defluvicoccus vanus gen. nov., sp. nov., a novel Gram-negative coccus/coccobacillus in the ‘Alphaproteobacteria’ from activated sludge. Int J Syst Evol Microbiol 55, 2105–2111.[CrossRef]
    [Google Scholar]
  27. Mehnaz, S., Weselowski, B. & Lazarovits, G. ( 2007a; ). Azospirillum canadense sp. nov., a nitrogen-fixing bacterium isolated from corn rhizosphere. Int J Syst Evol Microbiol 57, 620–624.[CrossRef]
    [Google Scholar]
  28. Mehnaz, S., Weselowski, B. & Lazarovits, G. ( 2007b; ). Azospirillum zeae sp. nov., a diazotrophic bacterium isolated from rhizosphere soil of Zea mays. Int J Syst Evol Microbiol 57, 2805–2809.[CrossRef]
    [Google Scholar]
  29. Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  30. Minnikin, D. E., O'Donnell, A. G., Goodfellow, M., Alderson, G., Athalye, M., Schaal, A. & Parlett, J. H. ( 1984; ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2, 233–241.[CrossRef]
    [Google Scholar]
  31. Nissen, H. & Dundas, I. D. ( 1984; ). Rhodospirillum salinarum sp. nov., a halophilic photosynthetic bacterium isolated from a Portuguese saltern. Arch Microbiol 138, 251–256.[CrossRef]
    [Google Scholar]
  32. Peng, G., Wang, H., Zhang, G., Hou, W., Liu, Y., Wang, E. T. & Tan, Z. ( 2006; ). Azospirillum melinis sp. nov., a group of diazotrophs isolated from tropical molasses grass. Int J Syst Evol Microbiol 56, 1263–1271.[CrossRef]
    [Google Scholar]
  33. Pfennig, N. & Trüper, H. G. ( 1971; ). Higher taxa of the phototrophic bacteria. Int J Syst Bacteriol 21, 17–18.[CrossRef]
    [Google Scholar]
  34. Reinhold, B., Hurek, T., Fendrik, I., Pot, B., Gillis, M., Kersters, K., Thielemans, S. & De Ley, J. ( 1987; ). Azospirillum halopraeferens sp. nov., a nitrogen-fixing organism associated with roots of Kallar Grass (Leptochloa fusca (L.) Kunth). Int J Syst Bacteriol 37, 43–51.[CrossRef]
    [Google Scholar]
  35. Richard, C. & Kiredjian, M. ( 1995; ). Laboratory Methods for the Identification of Strictly Aerobic Gram-negative Bacilli. Paris: Institut Pasteur.
  36. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  37. Sasser, M. ( 1990; ). Identification of bacteria by gas chromatography of cellular fatty acids. USFCC Newsl 20, 16
    [Google Scholar]
  38. Shi, B. H., Arunpairojana, V., Palakawong, S. & Yokota, A. ( 2002; ). Tistrella mobilis gen. nov., sp. nov., a novel polyhydroxyalkanoate producing bacterium belonging to a-Proteobacteria. J Gen Appl Microbiol 48, 335–343.[CrossRef]
    [Google Scholar]
  39. Sly, L. I. & Stackebrandt, E. ( 1999; ). Description of Skermanella parooensis gen. nov., sp. nov. to accommodate Conglomeromonas largomobilis subsp. parooensis following the transfer of Conglomeromonas largomobilis subsp. largomobilis to the genus Azospirillum. Int J Syst Bacteriol 49, 541–544.[CrossRef]
    [Google Scholar]
  40. Smibert, R. M. & Krieg, N. R. ( 1994; ). Phenotypic characterization. In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by P. Gerhardt, R. G. E. Murray, W. A. Wood & N. R. Krieg. Washington, DC: American Society for Microbiology.
  41. Tamaoka, J., Katayama-Fujimura, Y. & Kuraishi, H. ( 1983; ). Analysis of bacterial menaquinone mixtures by high performance liquid chromatography. J Appl Bacteriol 54, 31–36.[CrossRef]
    [Google Scholar]
  42. Tarrand, J. J., Krieg, N. R. & Döbereiner, J. ( 1978; ). A taxonomic study of the Spirillum lipoferum group, with description of a new genus, Azospirillum gen. nov., and two species, Azospirillum lipoferum (Beijerinck) comb. nov., and Azospirillum brasilense sp. nov. Can J Microbiol 24, 967–980.[CrossRef]
    [Google Scholar]
  43. Xie, C.-H. & Yokota, A. ( 2005; ). Azospirillum oryzae sp. nov., a nitrogen-fixing bacterium isolated from the roots of the rice plant Oryza sativa. Int J Syst Evol Microbiol 55, 1435–1438.[CrossRef]
    [Google Scholar]
  44. Young, C. C., Hupfer, H., Siering, C., Ho, M.-J., Arun, A. B., Lai, W.-A., Rekha, P. D., Shen, F.-T., Hung, M.-H. & other authors ( 2008; ). Azospirillum rugosum sp. nov., isolated from oil-contaminated soil. Int J Syst Evol Microbiol 58, 959–963.[CrossRef]
    [Google Scholar]
  45. Zhang, D., Yang, H., Zhang, W., Huang, Z. & Liu, S.-J. ( 2003; ). Rhodocista pekingensis sp. nov., a cyst-forming phototrophic bacterium from a municipal wastewater treatment plant. Int J Syst Evol Microbiol 53, 1111–1114.[CrossRef]
    [Google Scholar]
  46. Zhang, G. I., Hwang, C. Y. & Cho, B. C. ( 2008; ). Thalassobaculum litoreum gen. nov., sp. nov., a member of the family Rhodospirillaceae isolated from coastal seawater. Int J Syst Evol Microbiol 58, 479–485.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.009340-0
Loading
/content/journal/ijsem/10.1099/ijs.0.009340-0
Loading

Data & Media loading...

Supplements

Two-dimensional thin-layer chromatograms of the polar lipids of strains YIM D82 ( gen. nov., sp. nov.) and D812 ( sp. nov.). [PDF](24 KB)

PDF

Cellular fatty acid contents of strains YIM D82 ( gen. nov., sp. nov.) and D812 ( sp. nov.). [PDF](40 KB) (

PDF

Most cited articles

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error