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A nitrogen-fixing bacterium, designated strain X19-5T, was isolated from rhizosphere soil of Sonchus oleraceus. Phylogenetic analysis based on a fragment of the nifH gene and the full-length 16S rRNA gene sequence revealed that strain X19-5T was a member of the genus Paenibacillus. Strain X19-5T showed the highest 16S rRNA gene sequence similarity (98.8 %) with Paenibacillus graminis RSA19T and below 97 % similarity with other recognized members of the genus. The level of DNA–DNA relatedness between strain X19-5T and P. graminis RSA19T was 45.7 %. The DNA G+C content of strain X19-5T was 46.8 mol%. The major fatty acids were anteiso-C15 : 0, C16 : 0 and iso-C16 : 0. On the basis of its phenotypic characteristics and the level of DNA–DNA hybridization, strain X19-5T is considered to represent a novel species of the genus Paenibacillus, for which the name Paenibacillus sonchi sp. nov. is proposed. The type strain is X19-5T (=CCBAU 83901T=LMG 24727T).
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International Journal of Systematic and Evolutionary Microbiology vol. 59 , part 11, pp. 2656 - 2661
Supplementary Figure S1. Extended neighbour-joining tree of representatives of the genus Paenibacillus based on 16S rRNA gene sequences. [PDF](47 KB)