A nitrogen-fixing bacterium, designated strain X19-5, was isolated from rhizosphere soil of . Phylogenetic analysis based on a fragment of the gene and the full-length 16S rRNA gene sequence revealed that strain X19-5 was a member of the genus . Strain X19-5 showed the highest 16S rRNA gene sequence similarity (98.8 %) with RSA19 and below 97 % similarity with other recognized members of the genus. The level of DNA–DNA relatedness between strain X19-5 and RSA19 was 45.7 %. The DNA G+C content of strain X19-5 was 46.8 mol%. The major fatty acids were anteiso-C, C and iso-C. On the basis of its phenotypic characteristics and the level of DNA–DNA hybridization, strain X19-5 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is X19-5 (=CCBAU 83901=LMG 24727).


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  1. Ash, C., Priest, F. G. & Collins, M. D.(1993). Molecular identification of rRNA group 3 bacilli (Ash, Farrow, Wallbanks and Collins) using a PCR probe test. Proposal for the creation of a new genus Paenibacillus. Antonie Van Leeuwenhoek 64, 253–260. [Google Scholar]
  2. Berge, O., Guinebretiere, M. H., Achouak, W., Normand, P. & Heulin, T.(2002).Paenibacillus graminis sp. nov. and Paenibacillus odorifer sp. nov., isolated from plant roots, soil and food. Int J Syst Evol Microbiol 52, 607–616. [Google Scholar]
  3. Cowan, S. T. & Steel, K. J.(1965).Manual for the Identification of Medical Bacteria. London: Cambridge University Press.
  4. Ding, Y., Wang, J., Liu, Y. & Chen, S.(2005). Isolation and identification of nitrogen-fixing bacilli from plant rhizospheres in Beijing region. J Appl Microbiol 99, 1271–1281.[CrossRef] [Google Scholar]
  5. Elo, S., Suominen, I., Kampfer, P., Juhanoja, J., Salkinoja-Salonen, M. & Haahtela, K.(2001).Paenibacillus borealis sp. nov., a nitrogen-fixing species isolated from spruce forest humus in Finland. Int J Syst Evol Microbiol 51, 535–545. [Google Scholar]
  6. Gordon, R. E., Haynes, W. C. & Pang, C. H.-N.(1973).The Genus Bacillus. US Department of Agriculture Handbook no. 427. Washington, DC: Agricultural Research Service.
  7. Komagata, K. & Suzuki, K.(1987). Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19, 161–207. [Google Scholar]
  8. Kumar, S., Tamura, K. & Nei, M.(2004).mega3: integrated software for Molecular Evolutionary Genetics Analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef] [Google Scholar]
  9. Ma, Y. C. & Chen, S. F.(2008).Paenibacillus forsythiae sp. nov., a nitrogen-fixing species isolated from rhizosphere soil of Forsythia mira. Int J Syst Evol Microbiol 58, 319–323.[CrossRef] [Google Scholar]
  10. Ma, Y., Xia, Z., Liu, X. & Chen, S.(2007a).Paenibacillus sabinae sp. nov., a nitrogen-fixing species isolated from the rhizosphere soils of shrubs. Int J Syst Evol Microbiol 57, 6–11.[CrossRef] [Google Scholar]
  11. Ma, Y., Zhang, J. & Chen, S.(2007b).Paenibacillus zanthoxyli sp. nov., a novel nitrogen-fixing species isolated from the rhizosphere of Zanthoxyli simulans. Int J Syst Evol Microbiol 57, 873–877.[CrossRef] [Google Scholar]
  12. Priest, F. G., Goodfellow, M. & Todd, C.(1981). The genus Bacillus: a numerical analysis. In The Aerobic Endospore-Forming Bacteria. Classification and Identification, pp. 91–103. Edited by R. C. W. Berkeley & M. Goodfellow. London: Academic Press.
  13. Rhodes-Roberts, M.(1981). The taxonomy of some nitrogen-fixing Bacillus species with special reference to nitrogen fixation. In The Aerobic Endospore-Forming Bacteria. Classification and Identification, pp. 315–335. Edited by R. C. W. Berkeley & M. Goodfellow. London: Academic Press.
  14. Sambrook, J., Fritsch, E. F. & Maniatis, T.(1989).Molecular Cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
  15. Sasser, M., Kunitsky, C., Jackoway, G., Ezzell, J. W., Teska, J. D., Harper, B., Parker, S., Barden, D., Blair, H. & other authors(2005). Identification of Bacillus anthracis from culture using gas chromatographic analysis of fatty acid methyl esters. J AOAC Int 88, 178–181. [Google Scholar]
  16. Shida, O., Takagi, H., Kadowaki, K., Nakamura, L. K. & Komagata, K.(1997). Transfer of Bacillus alginolyticus, Bacillus chondroitinus, Bacillus curdlanolyticus, Bacillus glucanolyticus, Bacillus kobensis, and Bacillus thiaminolyticus to the genus Paenibacillus and emended description of the genus Paenibacillus. Int J Syst Bacteriol 47, 289–298.[CrossRef] [Google Scholar]
  17. Shirling, E. B. & Gottlieb, D.(1966). Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16, 313–340.[CrossRef] [Google Scholar]
  18. Suominen, I., Spröer, C., Kämpfer, P., Rainey, F. A., Lounatmaa, K. & Salkinoja-Salonen, M.(2003).Paenibacillus stellifer sp. nov., a cyclodextrin-producing species isolated from paperboard. Int J Syst Evol Microbiol 53, 1369–1374.[CrossRef] [Google Scholar]
  19. Tamaoka, J. & Komagata, K.(1984). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25, 125–128.[CrossRef] [Google Scholar]
  20. von der Weid, I., Frois Duarte, G., van Elsas, J. D. & Seldin, L.(2002).Paenibacillus brasilensis sp. nov., a novel nitrogen-fixing species isolated from the maize rhizosphere in Brazil. Int J Syst Evol Microbiol 52, 2147–2153.[CrossRef] [Google Scholar]
  21. Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A. D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray, R. G. E. & other authors(1987). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef] [Google Scholar]
  22. Weaver, P. F., Wall, J. D. & Gest, H.(1975). Characterization of Rhodopseudomonas capsulata. Arch Microbiol 105, 207–216.[CrossRef] [Google Scholar]
  23. Yanagi, M. & Yamasato, K.(1993). Phylogenetic analysis of the family Rhizobiaceae and related bacteria by sequencing of 16S rRNA gene using PCR and DNA sequencer. FEMS Microbiol Lett 107, 115–120.[CrossRef] [Google Scholar]
  24. Yoon, J.-H., Kim, H., Kim, S.-B., Kim, H.-J., Kim, W. Y., Lee, S. T., Goodfellow, M. & Park, Y.-H.(1996). Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. Int J Syst Bacteriol 46, 502–505.[CrossRef] [Google Scholar]
  25. Yoon, J. H., Oh, H. M., Yoon, B. D., Kang, K. H. & Park, Y. H.(2003).Paenibacillus kribbensis sp. nov. and Paenibacillus terrae sp. nov., bioflocculants for efficient harvesting of algal cells. Int J Syst Evol Microbiol 53, 295–301.[CrossRef] [Google Scholar]
  26. Ziemke, F., Höfle, M. G., Lalucat, J. & Rosselló-Mora, R.(1998). Reclassification of Shewanella putrefaciens Owen's genomic group II as Shewanella baltica sp. nov. Int J Syst Bacteriol 48, 179–186.[CrossRef] [Google Scholar]

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vol. , part 11, pp. 2656 - 2661

Extended neighbour-joining tree of representatives of the genus based on 16S rRNA gene sequences. [PDF](47 KB)

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