1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 59, Issue 9

sp. nov., sp. nov., sp. nov. and sp. nov. Free

Abstract

Bacteria isolated from eucalyptus leaves and shoots showing symptoms of blight and die-back collected in Uganda, Uruguay and Argentina and from maize displaying brown stalk rot symptoms in South Africa were tentatively placed in the genus on the basis of phenotypic and biochemical tests. These isolates, together with two strains (LMG 2558 and LMG 2560) previously assigned to based on protein electrophoregrams but later excluded from this species, were further investigated using molecular techniques. 16S rRNA gene sequencing and multilocus sequence analyses (MLSA) revealed that the strains were phylogenetically closely related to , and . MLSA and amplified fragment length polymorphism analysis placed the strains into four separate clusters, not containing any of the type strains of species of the genus . DNA–DNA hybridization confirmed the classification of the isolates into four novel species, for which the names sp. nov. (type strain R-21566 =LMG 24199 =BCC 105 =BD 765), sp. nov. (type strain R-25678 =LMG 24197 =BCC 076 =BD 769), sp. nov. (type strain R-31523 =LMG 24200 =BCC 109 =BD 767) and sp. nov. (type strain LMG 2558 =BD 871 =NCPPB 1682) are proposed.

  • Published Online:
Keyword(s): AFLP, amplified fragment length polymorphism and MLSA, multilocus sequence analysis
© SGM
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.009241-0
2009-09-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/59/9/2339.html?itemId=/content/journal/ijsem/10.1099/ijs.0.009241-0&mimeType=html&fmt=ahah

References

  1. Beji, A., Mergaert, J., Gavini, F., Izard, D., Kersters, K., Leclerc, H. & De Ley, J.(1988). Subjective synonymy of Erwinia herbicola, Erwinia milletiae, and Enterobacter agglomerans and redefinition of the taxon by genotypic and phenotypic data. Int J Syst Bacteriol 38, 77–88.[CrossRef] [Google Scholar]
  2. Brady, C. L., Venter, S. N., Cleenwerck, I., Vancanneyt, M., Swings, J. & Coutinho, T. A.(2007). A FAFLP system for the improved identification of plant-pathogenic and plant-associated species of the genus Pantoea. Syst Appl Microbiol 30, 413–417.[CrossRef] [Google Scholar]
  3. Brady, C. L., Cleenwerck, I., Venter, S. N., Vancanneyt, M., Swings, J. & Coutinho, T. A.(2008). Phylogeny and identification of Pantoea species associated with plants, humans and the natural environment based on multilocus sequence analysis (MLSA). Syst Appl Microbiol 31, 447–460.[CrossRef] [Google Scholar]
  4. Brenner, D. J., Fanning, G. R., Leete Knutson, J. K., Steigerwalt, A. G. & Krichevsky, M. I.(1984). Attempts to classify herbicola group-Enterobacter agglomerans strains by deoxyribonucleic acid hybridization and phenotypic tests. Int J Syst Bacteriol 34, 45–55.[CrossRef] [Google Scholar]
  5. Cleenwerck, I., Vandemeulebroecke, K., Janssens, D. & Swings, J.(2002). Re-examination of the genus Acetobacter, with descriptions of Acetobacter cerevisiae sp. nov. and Acetobacter malorum sp. nov. Int J Syst Evol Microbiol 52, 1551–1558.[CrossRef] [Google Scholar]
  6. Coenye, T., Falsen, E., Vancanneyt, M., Hoste, B., Govan, J. R. W., Kersters, K. & Vandamme, P.(1999). Classification of Alcaligenes faecalis-like isolates from the environment and human clinical samples as Ralstonia gilardii sp. nov. Int J Syst Bacteriol 49, 405–413.[CrossRef] [Google Scholar]
  7. Coutinho, T. A., Preisig, O., Mergaert, J., Cnockaert, M. C., Riedel, K. H., Swings, J. & Wingfield, M. J.(2002). Bacterial blight and dieback of Eucalyptus species, hybrids and clones in South Africa. Plant Dis 86, 20–25.[CrossRef] [Google Scholar]
  8. Ezaki, T., Hashimoto, Y. & Yabuuchi, E.(1989). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39, 224–229.[CrossRef] [Google Scholar]
  9. Gavini, F., Mergaert, J., Beji, A., Mielcarek, C., Izard, D., Kersters, K. & De Ley, J.(1989). Transfer of Enterobacter agglomerans (Beijerinck 1988) Ewing and Fife 1972 to Pantoea gen. nov. as Pantoea agglomerans comb. nov. and description of Pantoea dispersa sp. nov. Int J Syst Bacteriol 39, 337–345.[CrossRef] [Google Scholar]
  10. Goris, J., Suzuki, K., De Vos, P., Nakase, T. & Kersters, K.(1998). Evaluation of a microplate DNA-DNA hybridization method compared with the initial renaturation method. Can J Microbiol 44, 1148–1153.[CrossRef] [Google Scholar]
  11. Goszczynska, T., Botha, W. J., Venter, S. N. & Coutinho, T. A.(2007). Isolation and identification of the causal agent of brown stalk rot, a new disease of maize in South Africa. Plant Dis 91, 711–718.[CrossRef] [Google Scholar]
  12. Grimont, P. A. D. & Grimont, F.(2005). Genus XXIII. Pantoea Gavini, Mergaert, Beji, Mielcarek, Izard, Kersters and De Ley 1989b, 343VP. In Bergey's Manual of Systematic Bacteriology, 2nd edn, vol. 2, part B, pp. 713–720. Edited by D. J. Brenner, N. R. Krieg, J. T. Staley & G. M. Garrity. New York: Springer.
  13. Guindon, S. & Gascuel, O.(2003). A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood. Syst Biol 52, 696–704.[CrossRef] [Google Scholar]
  14. Kageyama, B., Nakae, M., Yagi, S. & Sonoyama, T.(1992).Pantoea punctata sp. nov., Pantoea citrea sp. nov., and Pantoea terrea sp. nov. isolated from fruit and soil samples. Int J Syst Bacteriol 42, 203–210.[CrossRef] [Google Scholar]
  15. Mergaert, J., Verdonck, L., Kersters, K., Swings, J., Boeufgras, J.-M. & De Ley, J.(1984). Numerical taxonomy of Erwinia species using API systems. J Gen Microbiol 130, 1893–1910. [Google Scholar]
  16. Mergaert, J., Verdonck, L. & Kersters, K.(1993). Transfer of Erwinia ananas (synonym, Erwinia uredovora) and Erwinia stewartii to the genus Pantoea emend. as Pantoea ananas (Serrano 1928) comb. nov. and Pantoea stewartii (Smith 1898) comb. nov., respectively, and description of Pantoea stewartii subsp. indologenes subsp. nov. Int J Syst Bacteriol 43, 162–173.[CrossRef] [Google Scholar]
  17. Mesbah, M., Premachandran, U. & Whitman, W. B.(1989). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef] [Google Scholar]
  18. Posada, D. & Crandall, K. A.(1998). Modeltest: testing the model of DNA substitution. Bioinformatics 14, 817–818.[CrossRef] [Google Scholar]
  19. Stephan, R., Van Trappen, S., Cleenwerck, I., Vancanneyt, M., De Vos, P. & Lehner, A.(2007).Enterobacter turicensis sp. nov. and Enterobacter helveticus sp. nov., isolated from fruit powder. Int J Syst Evol Microbiol 57, 820–826.[CrossRef] [Google Scholar]
  20. Swofford, D. L.(2000).paup*: phylogenetic analysis using parsimony and other methods. Sunderland, MA: Sinauer Associates.
  21. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G.(1997). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef] [Google Scholar]
  22. Verdonck, L., Mergaert, J., Rijckaert, C., Swings, J., Kersters, K. & De Ley, J.(1987). Genus Erwinia: numerical analysis of phenotypic features. Int J Syst Bacteriol 37, 4–18.[CrossRef] [Google Scholar]
  23. Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A. D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray, R. G. E. & other authors(1987). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef] [Google Scholar]
  24. Wilson, K.(1987). Preparation of genomic DNA from bacteria. In Current Protocols in Molecular Biology, pp. 2.4.1–2.4.5. Edited by F. M. Ausubel, R. Brent, R. E. Kingston, D. D. Moore, J. G. Seidman, J. A. Smith & K. Struhl. New York: Green Publishing & Wiley-Interscience.
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.009241-0
Loading
Pantoea vagans sp. nov., Pantoea eucalypti sp. nov., Pantoea deleyi sp. nov. and Pantoea anthophila sp. nov.
Int J Syst Evol Microbiol 59, 2339 (2009); https://doi.org/10.1099/ijs.0.009241-0
/content/journal/ijsem/10.1099/ijs.0.009241-0
/content/journal/ijsem/10.1099/ijs.0.009241-0
Loading

Data & Media loading...

Supplements

UPGMA dendrogram based on FAFLP analysis of selected strains using the selective primer combination Eco-C/Mse-GC. [ PDF] 314 KB

PDF

DNA–DNA hybridization amongst strains of sp. nov., sp. nov., sp. nov. and sp. nov. and reference strains of species of the genus with validly published names. [ PDF] 143 KB

PDF

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error