A Gram-negative, aerobic, rod-shaped, motile, marine bacterium, strain AR11, was isolated from Arctic marine sediment. Strain AR11 grew with 0.5–7 % NaCl and at 7–37 °C and pH 5.5–9.0. It utilized propionate, 3-hydroxybenzoate, -proline, acetate, - and -lactate, -alanine, malate and phenylacetic acid. Alkaline phosphatase, esterase lipase (C8), leucine arylamidase and acid phosphatase activity tests were positive. Acid was produced from 5-ketogluconate and aesculin. Strain AR11 possessed C (22.0 %), summed feature 4 (C 7 and/or iso-C 2-OH; 28.1 %) and summed feature 7 (one or more of C 7, 9 and 12; 34.0 %) as the major cellular fatty acids. The major ubiquinone was Q-8. Comparative 16S rRNA gene sequence studies showed that strain AR11 belonged to the and was most closely related to DSM 7027, mano11 and KW-40 (97.8, 97.0 and 96.7 % similarity, respectively). The G+C content of the genomic DNA of strain AR11 was 57.9 mol%. DNA–DNA relatedness data indicated that strain AR11 represented a distinct species that was separated from DSM 7027, KCTC 12240 and JCM 21667. On the basis of evidence from this polyphasic study, it is proposed that strain AR11 (=KCTC 22254=JCM 15134) represents the type strain of a novel species, sp. nov.


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vol. , part 12, pp. 3030 - 3034

Phylogenetic relationships of isolate AR11 and some related micro-organisms on the basis of 16S rRNA gene sequences. [PDF](62 KB)

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