1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 59, Issue 9

sp. nov., an endophytic actinomycete isolated from the stem of Free

Abstract

A novel actinomycete strain, YIM 61503, isolated from the stem of , was characterized by using a polyphasic approach. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain YIM 61503 belonged to the genus and exhibited 16S rRNA gene sequence similarities of 98.8 and 98.6 % to D8-87 and YIM 002. The chemotaxonomic properties of strain YIM 61503 were consistent with those of the genus : the cell-wall peptidoglycan type was based on -diaminopimelic acid and MK-9(H) was the predominant menaquinone. The major fatty acids were anteiso-C, iso-C, iso-C, C 8 and anteiso-C. The DNA G+C content was 71.9 mol%. Strain YIM 61503 was phenotypically distinct from recognized species and was shown to belong to a separate genomic species based on DNA–DNA hybridization results. Thus, strain YIM 61503 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is YIM 61503 (=DSM 45237 =CCTCC AA 208023).

  • Published Online:
Keyword(s): DAP, 2,6-diaminopimelic acid
© SGM
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.009001-0
2009-09-01
2024-04-24
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/59/9/2162.html?itemId=/content/journal/ijsem/10.1099/ijs.0.009001-0&mimeType=html&fmt=ahah

References

  1. Collins, M. D. & Jones, D.(1980). Lipids in the classification and identification of coryneform bacteria containing peptidoglycans based on 2,4-diaminobutyric acid. J Appl Bacteriol 48, 459–470.[CrossRef] [Google Scholar]
  2. Collins, M. D., Pirouz, T., Goodfellow, M. & Minnikin, D. E.(1977). Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100, 221–230.[CrossRef] [Google Scholar]
  3. Ezaki, T., Hashimoto, Y. & Yabuuchi, E.(1989). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39, 224–229.[CrossRef] [Google Scholar]
  4. Felsenstein, J.(1985). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef] [Google Scholar]
  5. Gordon, R. E., Barnett, D. A., Handerhan, J. E. & Pang, C. H.-N.(1974).Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. Int J Syst Bacteriol 24, 54–63.[CrossRef] [Google Scholar]
  6. Groth, I., Schumann, P., Rainey, F. A., Martin, K., Schuetze, B. & Augsten, K.(1997).Demetria terragena gen. nov., sp. nov., a new genus of actinomycetes isolated from compost soil. Int J Syst Bacteriol 47, 1129–1133.[CrossRef] [Google Scholar]
  7. Hasegawa, T., Takizawa, M. & Tanida, S.(1983). A rapid analysis for chemical grouping of aerobic actinomycetes. J Gen Appl Microbiol 29, 319–322.[CrossRef] [Google Scholar]
  8. He, L., Li, W., Huang, Y., Wang, L., Liu, Z., Lanoot, B., Vancanneyt, M. & Swings, J.(2005).Streptomyces jietaisiensis sp. nov., isolated from soil in northern China. Int J Syst Evol Microbiol 55, 1939–1944.[CrossRef] [Google Scholar]
  9. Kämpfer, P. & Kroppenstedt, R. M.(1996). Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 42, 989–1005.[CrossRef] [Google Scholar]
  10. Kimura, M.(1980). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16, 111–120.[CrossRef] [Google Scholar]
  11. Kluge, A. G. & Farris, J. S.(1969). Quantitative phyletics and the evolution of anurans. Syst Zool 18, 1–32.[CrossRef] [Google Scholar]
  12. Kumar, S., Tamura, K. & Nei, M.(2004).mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef] [Google Scholar]
  13. Lee, S. D.(2008).Jiangella alkaliphila sp. nov., an actinobacterium isolated from a cave. Int J Syst Evol Microbiol 58, 1176–1179.[CrossRef] [Google Scholar]
  14. Li, W. J., Xu, P., Schumann, P., Zhang, Y. Q., Pukall, R., Xu, L. H., Stackebrandt, E. & Jiang, C. L.(2007).Georgenia ruanii sp. nov., a novel actinobacterium isolated from forest soil in Yunnan (China), and emended description of the genus Georgenia. Int J Syst Evol Microbiol 57, 1424–1428.[CrossRef] [Google Scholar]
  15. Mesbah, M., Premachandran, U. & Whitman, W. B.(1989). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef] [Google Scholar]
  16. Minnikin, D. E., Collins, M. D. & Goodfellow, M.(1979). Fatty acid and polar lipid composition in the classification of Cellulomonas, Oerskovia and related taxa. J Appl Bacteriol 47, 87–95.[CrossRef] [Google Scholar]
  17. Minnikin, D. E., Hutchinson, I. G., Caldicott, A. B. & Goodfellow, M.(1980). Thin layer chromatography of methanolysates of mycolic acid-containing bacteria. J Chromatogr 188, 221–233.[CrossRef] [Google Scholar]
  18. Otoguro, M., Hayakawa, M., Yamazaki, T. & Iimura, Y.(2001). An integrated method for the enrichment and selective isolation of Actinokineospora spp. in soil and plant litter. J Appl Microbiol 91, 118–130.[CrossRef] [Google Scholar]
  19. Qin, S., Wang, H. B., Chen, H. H., Zhang, Y. Q., Jiang, C. L., Xu, L. H. & Li, W. J.(2008).Glycomyces endophyticus sp. nov., an endophytic actinomycete isolated from the root of Carex baccans Nees. Int J Syst Evol Microbiol 58, 2525–2528.[CrossRef] [Google Scholar]
  20. Saitou, N. & Nei, M.(1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425. [Google Scholar]
  21. Sasser, M.(1990).Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
  22. Shirling, E. B. & Gottlieb, D.(1966). Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16, 313–340.[CrossRef] [Google Scholar]
  23. Song, L., Li, W. J., Wang, Q. L., Chen, G. Z., Zhang, Y. S. & Xu, L. H.(2005).Jiangella gangsuensis gen. nov., sp. nov., a novel actinomycete from a desert soil in north-west China. Int J Syst Evol Microbiol 55, 881–884.[CrossRef] [Google Scholar]
  24. Stackebrandt, E. & Goebel, B. M.(1994). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef] [Google Scholar]
  25. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G.(1997). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef] [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.009001-0
Loading
Jiangella alba sp. nov., an endophytic actinomycete isolated from the stem of Maytenus austroyunnanensis
Int J Syst Evol Microbiol 59, 2162 (2009); https://doi.org/10.1099/ijs.0.009001-0
/content/journal/ijsem/10.1099/ijs.0.009001-0
/content/journal/ijsem/10.1099/ijs.0.009001-0
Loading

Data & Media loading...

Supplements

vol. , part 9, pp. 2162 - 2165

Cellular fatty acid profiles of sp. nov. YIM 61503 and other type strains of the genus . [PDF](64 KB)

PDF

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error