1887

Abstract

A Gram-negative, non-spore-forming, cream-coloured bacterial strain, UM2, was isolated from an open hexachlorocyclohexane (HCH) dump site at Ummari village in Lucknow, India. Data generated from a polyphasic approach including phenotypic, genotypic and chemotaxonomic analyses confirmed that strain UM2 belonged to the genus . The highest similarity found to the 16S rRNA gene sequence of strain UM2 was 99.4 %, with DSM 6014, whereas the DNA–DNA relatedness value between these strains was 31 %, indicating that they represent separate species. The DNA G+C content of UM2 was 66.9 mol%. The respiratory pigment ubiquinone Q-10 was present. The predominant fatty acids were summed feature 8 (C 6 and/or C 7; 32.9 %), C cyclo 8 (15.5 %) and C (12.1 %). The major polar lipids were phosphatidylcholine, phosphatidylglycerol and phosphatidyldimethylethanolamine. -Homospermidine was the major polyamine observed. On the basis of the data reported, it was concluded that UM2 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is UM2 (=MTCC 9473 =CCM 7545).

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2010-05-01
2019-10-20
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Transmission electron micrograph of a negatively stained cell of strain UM2 showing polar flagellation. Bar, 1 µm.

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