1887

Abstract

Five Gram-stain-positive, motile, aerobic strains were isolated from a clean room of the Kennedy Space Center where the Phoenix spacecraft was assembled. All strains are rod-shaped, spore-forming bacteria, whose spores were resistant to UV radiation up to 1000 J m. The spores were subterminally positioned and produced an external layer. A polyphasic taxonomic study including traditional biochemical tests, fatty acid analysis, cell-wall typing, lipid analyses, 16S rRNA gene sequencing and DNA–DNA hybridization studies was performed to characterize these novel strains. 16S rRNA gene sequencing and lipid analyses convincingly grouped these novel strains within the genus as a cluster separate from already described species. The similarity of 16S rRNA gene sequences among the novel strains was >99 %, but the similarity was only about 97 % with their nearest neighbours , and . DNA–DNA hybridization dissociation values were <24 % to the closest related type strains. The novel strains had a G+C content 35.6±0.5 mol% and could liquefy gelatin but did not utilize or produce acids from any of the carbon substrates tested. The major fatty acids were iso-C and anteiso-C and the cell-wall diamino acid was meso-diaminopimelic acid. Based on phylogenetic and phenotypic results, it is concluded that these strains represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is 1P01SC (=NRRL B-59162 =MTCC 9535).

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2010-05-01
2019-09-16
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Micrographs of sp. nov. 1P01SC . (a) Light micrograph (phase-contrast) showing vegetative cells with and without subterminal spores and free spores. Bar, 2.5 µm. (b) Scanning electron micrograph showing spores with an external layer that enables spores to connect to each other. Bar, 3.0 µm. (c) Transmission electron microscopy of a 50 nm section showing loosely attached external layers around the spore coat. Bar, 200 nm. (d) Higher-magnification scanning electron micrograph of endospores, revealing the highly integrated slimy external layer with spores. Bar, 500 nm.

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Two-dimensional TLC of polar lipids of sp. nov. 1P01SC (A), sp. nov. 1P08SA (B) and DSM 10 (C; reproduced with permission from Kämpfer , 2006). DPG, Diphosphatidylglycerol; PG, phosphatidylglycerol; PE, phosphatidylethanolamine; PL1–10, unknown phospholipids; PN2, unknown aminophospholipid; GL1, β-gentiobiosyldiacylglycerol; PG, phosphatidylglycerol; PE, phosphatidylethanolamine; APL, unknown aminophospholipid.

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rep-PCR-based DNA fingerprinting of sp. nov. isolates. Grouping of the isolates depicted in the cladogram on the left was based on the gel electrophoresis band patterns on the right.

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