1887

Abstract

Ten strains of an asexual arthroconidial yeast species were isolated from Bryndza, a traditional Slovak artisanal sheep cheese, which was manufactured from raw milk during a 4-month summer production period at two Slovakian sites (the northern Ružomberok and the central-southern Tisovec areas). Sequence comparison of the D1/D2 domains of the large-subunit rRNA gene revealed that this yeast represents a novel species of the genus , which contains anamorphs of the ascogenous genus , for which the name sp. nov. is proposed (type culture CCY 16-2-1 =NRRL Y-48450 =CBS 11176). The novel species is most closely related to NRRL Y-27641, although yeasts with identical or very similar sequences have been found throughout the world.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.008938-0
2009-09-01
2020-09-18
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/59/9/2370.html?itemId=/content/journal/ijsem/10.1099/ijs.0.008938-0&mimeType=html&fmt=ahah

References

  1. Altschul, S. F., Gish, W., Miller, W., Myers, E. W. & Lipman, D. J. ( 1990; ). Basic local alignment search tool. J Mol Biol 215, 403–410.[CrossRef]
    [Google Scholar]
  2. Altschul, S. F., Madden, T. L., Schäffer, A. A., Zhang, J., Zhang, Z., Miller, W. & Lipman, D. J. ( 1997; ). Gapped blast and psi-blast: a new generation of protein database search programs. Nucleic Acids Res 25, 3389–3402.[CrossRef]
    [Google Scholar]
  3. Barnett, J. A., Payne, R. W. & Yarrow, D. ( 2000; ). In Yeasts: Characteristics and Identification, 3rd edn, pp. 388–394. Cambridge: Cambridge University Press.
  4. Boutrou, R. & Guéguen, M. ( 2005; ). Interests in Geotrichum candidum for cheese technology. Int J Food Microbiol 102, 1–20.[CrossRef]
    [Google Scholar]
  5. Defontaine, A., Lecocq, F. M. & Hallet, J. N. ( 1991; ). A rapid miniprep method for the preparation of yeast mitochondrial DNA. Nucleic Acids Res 19, 185 [CrossRef]
    [Google Scholar]
  6. de Hoog, G. S. & Smith, M. T. ( 2004; ). Ribosomal gene phylogeny and species delimitation in Geotrichum and its teleomorphs. Stud Mycol 50, 489–515.
    [Google Scholar]
  7. de Hoog, G. S., Smith, M. T. & Guého, E. ( 1986; ). A revision of the genus Geotrichum and its teleomorphs. Stud Mycol 29, 1–131.
    [Google Scholar]
  8. de Hoog, G. S., Smith, M. T. & Guého, E. ( 1998a; ). Dipodascus de Lagerheim. In The Yeasts, a Taxonomic Study, 4th edn, pp. 181–193. Edited by C. P. Kurtzman & J. W. Fell. Amsterdam: Elsevier.
  9. de Hoog, G. S., Smith, M. T. & Guého, E. ( 1998b; ). Galactomyces Readhead & Malloch. In The Yeasts, a Taxonomic Study, 4th edn, pp. 209–213. Edited by C. P. Kurtzman & J. W. Fell. Amsterdam: Elsevier.
  10. Desmasures, N., Bazin, F. & Guéguen, M. ( 1997; ). Microbiological composition of raw milk from selected farms in the Camembert region of Normandy. J Appl Microbiol 83, 53–58.[CrossRef]
    [Google Scholar]
  11. Fell, J. W., Boekhout, T., Fonseca, A., Scorzetti, G. & Statzell-Tallman, A. ( 2000; ). Biodiversity and systematics of basidiomycetous yeasts as determined by large-subunit rDNA D1/D2 domain sequence analysis. Int J Syst Evol Microbiol 50, 1351–1371.[CrossRef]
    [Google Scholar]
  12. Felsenstein, J. ( 1988; ). Phylogenies from molecular sequences: inference and reliability. Annu Rev Genet 22, 521–565.[CrossRef]
    [Google Scholar]
  13. Kurtzman, C. P. ( 2006; ). Yeast species recognition from gene sequence analyses and other molecular methods. Mycoscience 47, 65–71.[CrossRef]
    [Google Scholar]
  14. Kurtzman, C. P. & Fell, J. W. (editors) ( 1998; ). The Yeasts: a Taxonomic Study, 4th edn. Amsterdam: Elsevier.
  15. Kurtzman, C. P. & Robnett, C. J. ( 1998; ). Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences. Antonie van Leeuwenhoek 73, 331–371.[CrossRef]
    [Google Scholar]
  16. Lachance, M. A., Daniel, H. M., Meyer, W., Prasad, G. S., Gautam, S. P. & Boundy-Mills, K. ( 2003; ). The D1/D2 domain of the large subunit rDNA of the yeast species Clavispora lusitaniae is unusually polymorphic. FEMS Yeast Res 4, 253–258.[CrossRef]
    [Google Scholar]
  17. Laurenčík, M., Sulo, P., Sláviková, E., Piecková, E., Seman, M. & Ebringer, L. ( 2008; ). The diversity of eukaryotic microbiota in the traditional Slovak sheep cheese – Bryndza. Int J Food Microbiol 127, 176–179.[CrossRef]
    [Google Scholar]
  18. Marinoni, G., Manuel, M., Petersen, R. F., Hvidtfeldt, J., Sulo, P. & Piškur, J. ( 1999; ). Horizontal transfer of genetic material among Saccharomyces yeasts. J Bacteriol 181, 6488–6496.
    [Google Scholar]
  19. Naumova, E. S., Smith, M. Th., Boekhout, T., de Hoog, G. S. & Naumov, G. I. ( 2001; ). Molecular differentiation of sibling species in the Galactomyces geotrichum complex. Antonie van Leeuwenhoek 80, 263–273.[CrossRef]
    [Google Scholar]
  20. Pimenta, R. S., Alves, P. D. D., Corrêa, A., Jr, Lachance, M. A., Prasad, G. S., Rajaram, Sinha, B. R. & Rosa, C. A. ( 2005; ). Geotrichum silvicola sp. nov., a novel asexual arthroconidial yeast species related to the genus Galactomyces. Int J Syst Evol Microbiol 55, 497–501.[CrossRef]
    [Google Scholar]
  21. Smith, M. Th. & Poot, G. A. ( 2003; ). Genome comparisons in the genus Dipodascus de Lagerheim. FEMS Yeast Res 3, 301–311.[CrossRef]
    [Google Scholar]
  22. Smith, M. Th., Poot, G. A. & de Cock, A. W. A. M. ( 2000; ). Re-examination of some species of the genus Geotrichum Link: Fr. Antonie van Leeuwenhoek 77, 71–81.[CrossRef]
    [Google Scholar]
  23. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  24. Ueda-Nishimura, K. & Mikata, K. ( 2000; ). Two distinct 18S rRNA secondary structures in Dipodascus (Hemiascomycetes). Microbiology 146, 1045–1051.
    [Google Scholar]
  25. Wuczkowski, M., Bond, C. & Prillinger, H. ( 2006; ). Geotrichum vulgare sp. nov., a novel asexual arthroconidial yeast. Int J Syst Evol Microbiol 56, 301–303.[CrossRef]
    [Google Scholar]
  26. Yarrow, D. ( 1998; ). Methods for the isolation, maintenance and identification of yeasts. In The Yeasts, a Taxonomic Study, 4th edn, pp. 77–100. Edited by C. P. Kurtzman & J. W. Fell. Amsterdam: Elsevier.
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.008938-0
Loading
/content/journal/ijsem/10.1099/ijs.0.008938-0
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error