In this report, we describe three novel asexual basidiomycetous yeast species, sp. nov. (type strain CBS 10872 =SDY 081), sp. nov. (type strain CBS 10871=SDY 022) and sp. nov. (type strain CBS 10873 =SDY 190), which were isolated from acid rock drainage collected at the São Domingos mine in southern Portugal. Phylogenetic analysis of molecular sequence data indicated that the novel species belong to the order Filobasidiales of the class Tremellomycetes and form a well-separated clade, next to and . Since the novel species also share a peculiar ecology, being able to thrive under extreme environmental conditions characterized by very low pH and high concentrations of heavy metals, we designate this combination of phylogenetic and ecological characteristics as an ecoclade.


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  1. Amaral Zettler, L. A., Gómez, F., Zettler, E., Keenan, B. G., Amils, R. & Sogin, M. L.(2002). Eukaryotic diversity in Spain's river of fire. Nature 417, 137[CrossRef] [Google Scholar]
  2. Beck, J. V.(1960). A ferrous-ion-oxidizing bacterium. I. Isolation and some general physiological characteristics. J Bacteriol 79, 502–509. [Google Scholar]
  3. Bryner, L. C., Beck, J. V., Davis, D. B. & Wilson, D. G.(1954). Microorganisms in leaching sulphide minerals. Ind Eng Chem 46, 2587–2592.[CrossRef] [Google Scholar]
  4. Ehrlich, H. L.(1963). Microorganisms in acid drainage from a copper mine. J Bacteriol 86, 350–352. [Google Scholar]
  5. Felsenstein, J.(1985). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef] [Google Scholar]
  6. Fonseca, A.(1992). Utilization of tartaric acid and related compounds by yeasts: taxonomic implications. Can J Microbiol 38, 1242–1251.[CrossRef] [Google Scholar]
  7. Gadanho, M., Libkind, D. & Sampaio, J. P.(2006). Yeast diversity in the extreme acidic environments of the Iberian pyrite belt. Microb Ecol 52, 552–563.[CrossRef] [Google Scholar]
  8. Kimura, M.(1980). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16, 111–120.[CrossRef] [Google Scholar]
  9. López-Archilla, A. I., Marin, I. & Amils, R.(2001). Microbial community composition and ecology of an acidic aquatic environment: the Tinto River, Spain. Microb Ecol 41, 20–35. [Google Scholar]
  10. Margesin, R., Fonteyne, P.-A., Schinner, F. & Sampaio, J. P.(2007).Rhodotorula psychrophila sp. nov., Rhodotorula psychrophenolica sp. nov. and Rhodotorula glacialis sp. nov., novel psychrophilic basidiomycetous yeast species isolated from alpine environments. Int J Syst Evol Microbiol 57, 2179–2184.[CrossRef] [Google Scholar]
  11. Russo, G., Libkind, D., Sampaio, J. P. & van Broock, M. R.(2008). Yeast diversity in the acidic Rio Agrio-Lake Caviahue volcanic environment (Patagonia, Argentina). FEMS Microbiol Ecol 65, 415–424.[CrossRef] [Google Scholar]
  12. Saitou, N. & Nei, M.(1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425. [Google Scholar]
  13. Sampaio, J. P.(1999). Utilization of low molecular weight aromatic compounds by heterobasidiomycetous yeasts: taxonomic implications. Can J Microbiol 45, 491–512.[CrossRef] [Google Scholar]
  14. Sampaio, J. P.(2004). Diversity, phylogeny and classification of basidiomycetous yeasts. In Frontiers in Basidiomycete Mycology, pp. 49–80. Edited by R. Agerer, M. Piepenbring & P. Blanz. Eching, Germany: IHW-Verlag.
  15. Scorzetti, G., Fell, J. W., Fonseca, A. & Statzell-Tallman, A.(2002). Systematics of basidiomycetous yeasts: a comparison of large subunit D1D2 and internal transcribed spacer rDNA regions. FEMS Yeast Res 2, 495–517.[CrossRef] [Google Scholar]
  16. Swofford, D. L.(2001).paup*: phylogenetic analysis using parsimony (*and other methods). Sunderland, MA: Sinauer Associates.
  17. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G.(1997). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef] [Google Scholar]
  18. Yarrow, D.(1998). Methods for the isolation, maintenance and identification of yeasts. In The Yeasts, a Taxonomic Study, 4th edn, pp. 77–100. Edited by C. P. Kurtzman & J. W. Fell. Amsterdam: Elsevier.

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