A psychrotrophic strain, GP3, was isolated from Adélie penguin guano from Antarctica and characterized. The 16S rRNA gene sequence of the novel strain showed the highest similarity (97.8 %) with that of B7. The novel strain showed a morphological change from rod to coccus. The growth temperature range of strain GP3 was 0–25 °C, with optimal growth at 20 °C. The strain grew over a salinity range of between 0 and 3 % (w/v) NaCl and the optimal pH for growth was pH 6–8. Strain GP3 contained anteiso-C as the major fatty acid. The major menaquinone was MK-9(H). The cell-wall peptidoglycan type was of the A3 variant. The DNA G+C content of strain GP3 was 58.5 mol%. Strain GP3 was able to hydrolyse chitin, Tween 80, starch, cellulose and lactose, but not gelatin, lecithin or urea. In addition to these physiological characteristics, DNA–DNA hybridization studies clearly differentiated strain GP3 from . The combined results of phylogenetic, physiological and chemotaxonomic studies indicated that strain GP3 represents a novel species of the genus . The name sp. nov. (type strain GP3=JCM 13874=CGMCC 1.6355) is proposed in recognition of the strain's strong chitin-utilizing ability.


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  1. Chen, M., Xiao, X., Wang, P., Zeng, X. & Wang, F.(2005).Arthrobacter ardleyensis sp. nov., isolated from Antarctic lake sediment and deep-sea sediment. Arch Microbiol 183, 301–305.[CrossRef] [Google Scholar]
  2. Collins, M. D., Pirouz, T., Goodfellow, M. & Minnikin, D. E.(1977). Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100, 221–230.[CrossRef] [Google Scholar]
  3. Dong, X. Z. & Cai, M. Y.(2001).Manual of Systematic Identification of Familiar Bacteria, 1st edn. Beijing, China: Science Publishing Company
  4. Jones, D. & Keddie, R. M.(1992). The genus Arthrobacter. In The Prokaryotes, vol. 2, pp. 1283–1299. Edited by A. Balows, H. G. Trüper, M. Dworkin, W. Harder & K. H. Schleifer. Berlin, Heidelberg, New York: Springer.
  5. Keddie, R. M., Collins, D. & Jones, D.(1986). Genus Arthrobacter. In Bergey's Manual of Systematic Bacteriology, vol. 2, pp. 1261–1266. Edited by P. H. A. Sneath, N. S. Mair, M. E. Sharpe & J. G. Holt. Baltimore: Williams & Wilkins.
  6. Kumar, S., Tamura, K. & Nei, M.(2004).mega3: integrated software for Molecular Evolutionary Genetics Analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef] [Google Scholar]
  7. Loveland-Curtze, J., Sheridan, P. P., Gutshall, K. R. & Brenchley, J. E.(1999). Biochemical and phylogenetic analyses of psychrophilic isolates belonging to the Arthrobacter subgroup and description of Arthrobacter psychrolactophilus, sp. nov. Arch Microbiol 171, 355–363.[CrossRef] [Google Scholar]
  8. Margesin, R., Schumann, P., Spröer, C. & Gounot, A. M.(2004).Arthrobacter psychrophenolicus sp. nov., isolated from an alpine ice cave. Int J Syst Evol Microbiol 54, 2067–2072.[CrossRef] [Google Scholar]
  9. Mavromatis, K., Lorito, M., Woo, S. L. & Bouriotis, V.(2003). Mode of action and antifungal properties of two cold-adapted chitinases. Extremophiles 7, 385–390.[CrossRef] [Google Scholar]
  10. Mesbah, M., Premachandran, U. & Whitman, W. B.(1989). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef] [Google Scholar]
  11. Nishijima, M., Araki-Sakai, M. & Sano, H.(1997). Identification of isoprenoid quinones by frit-FAB liquid chromatography-mass spectrometry for the chemotaxonomy of microorganisms. J Microbiol Methods 28, 113–122.[CrossRef] [Google Scholar]
  12. Reddy, G. S. N., Aggarwal, R. K., Matsumoto, G. I. & Shivaji, S.(2000).Arthrobacter flavus sp. nov., a psychrophilic bacterium isolated from a pond in McMurdo Dry Valley, Antarctica. Int J Syst Evol Microbiol 50, 1553–1561.[CrossRef] [Google Scholar]
  13. Reddy, G. S. N., Prakash, J. S. S., Matsumoto, G. I., Stackebrandt, E. & Shivaji, S.(2002).Arthrobacter roseus sp. nov., a psychrophilic bacterium isolated from an Antarctic cyanobacterial mat sample. Int J Syst Evol Microbiol 52, 1017–1021.[CrossRef] [Google Scholar]
  14. Schleifer, K. H. & Kandler, O.(1972). Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36, 407–477. [Google Scholar]
  15. Skalova, T., Dohnalek, J., Spiwok, V., Lipovova, P., Vondrackova, E., Petrokova, H., Duskova, J., Strnad, H., Kralova, B. & Hasek, J.(2005). Cold-active β-galactosidase from Arthrobacter sp. C2-2 forms compact 660 kDa hexamers: crystal structure at 1.9 Å resolution. J Mol Biol 353, 282–294.[CrossRef] [Google Scholar]
  16. Stackebrandt, E., Fowler, V. J., Fiedler, F. & Seiler, H.(1983). Taxonomic studies on Arthrobacter nicotianae and related taxa: description of Arthrobacter uratoxydans sp. nov. and Arthrobacter sulfureus sp. nov. and reclassification of Brevibacterium protophormiae as Arthrobacter protophormiae comb. nov. Syst Appl Microbiol 4, 470–486.[CrossRef] [Google Scholar]
  17. Tamaoka, J. & Komagata, K.(1984). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25, 125–128.[CrossRef] [Google Scholar]
  18. Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A. D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray, R. G. E. & other authors(1987). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef] [Google Scholar]
  19. Xiao, X., Yin, X., Lin, J., Sun, L., You, Z., Wang, P. & Wang, F.(2005). Chitinase genes in lake sediments of Ardley Island, Antarctica. Appl Environ Microbiol 71, 7904–7909.[CrossRef] [Google Scholar]
  20. Xiao, X., Wang, P., Zeng, X., Bartlett, D. H. & Wang, F. P.(2007).Shewanella psychrophila sp. nov., Shewanella piezotolerans sp. nov., isolated from west Pacific deep-sea sediment. Int J Syst Evol Microbiol 57, 60–65.[CrossRef] [Google Scholar]

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