Two actinomycete strains, NRRL B-24165 and NRRL B-24166, isolated from lesions on equine placentas in Kentucky, USA, were analysed using a polyphasic taxonomic approach. On the basis of phylogenetic analysis of 16S rRNA gene sequences, morphological observations and the presence of -diaminopimelic acid as the diagnostic diamino acid in whole-cell hydrolysates, the new isolates clearly belonged to the genus . Analyses of the phylogenetic positions of strains NRRL B-24165 and NRRL B-24166 based on 16S rRNA gene sequences of all recognized species of the genus , as well as evaluation of morphological and physiological characteristics, demonstrated that the new isolates could be differentiated from all recognized species and therefore represented novel species. It is proposed that the new strains represent two novel species for which the names sp. nov. (type strain NRRL B-24165=DSM 41860=LDDC 6330-99) and sp. nov. (NRRL B-24166=DSM 41861=LDDC 6638-99) are proposed. The species names are based on the distinctive colours of the substrate mycelium of these strains, dark red and deep orange–yellow, respectively.


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MALDI-TOF MS ion profiles of strains B-24165, B-24676 and B-24679. [ PDF] 38 KB


Morphological characteristics of sp. nov. and sp. nov. compared with the phylogenetically nearest species. [ PDF] 58 KB


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