The complex (MAC) consists of four recognized species, , , and , and a variety of other strains that may be members of undescribed taxa. We report on two isolates of a scotochromogenic, slowly growing, non-tuberculous species within the complex from a lymph node and an infected wound after a dogbite of separate patients in The Netherlands. The extrapulmonary infections in immunocompetent patients suggested a high level of virulence. These isolates were characterized by a unique nucleotide sequence in the 16S rRNA gene, 99 % similar to , and the MAC-Q 16S–23S internal transcribed spacer (ITS) sequence. Sequence analyses of the gene revealed 97 % similarity to . The gene sequence was 98 % similar to . Phenotypically, the scotochromogenicity, positive semi-quantitative catalase and heat-stable catalase tests, negative tellurite reductase and urease tests and susceptibility to hydroxylamine and oleic acid set these isolates apart from related species. High-performance liquid chromatography analysis of cell-wall mycolic acid content revealed a unique pattern, related to that of and . Together, these findings supported a separate species status within the complex. We propose elevation of scotochromogenic complex strains sharing this 16S gene and MAC-Q ITS sequence to separate species status, for which the name sp. nov. is proposed. The type strain is NLA000700772 (=DSM 45247=CIP 109859).


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Phylogenetic tree based on gene sequences. [PDF](28 KB)


Phylogenetic tree based on gene sequences. [PDF](35 KB)


Phylogenetic tree based on concatenated 16S, and gene sequences. [PDF](17 KB)

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