1887

Abstract

A novel yeast species, sp. nov., is proposed in this paper based on two strains isolated from leaves and one strain from soil in Taiwan. The strains produce one or two spherical ascospores in each ascus, preceded by conjugation of individual cells. Genus assignment and distinction of the species from other recognized species of are based on morphological and physiological characteristics and on phylogenetic analysis of nucleotide sequences of the D1/D2 domains of the large subunit (LSU) rRNA gene. Sequence analysis of the D1/D2 domains of the LSU rRNA gene reveals that the phylogenetically closest relatives of sp. nov. are and . The novel species could be differentiated from the latter two species based on electrophoretic karyotypes. The type strain of sp. nov. is SC5L02 (=CBS 10888 =BCRC 23139), which was isolated from a leaf of Rosenst. in Dasi, Taoyuan, Taiwan.

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2009-07-01
2019-12-07
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References

  1. Barnett, J. A., Payne, R. W. & Yarrow, D. ( 2000; ). Yeasts: Characteristics and Identification, 3rd edn. Cambridge: Cambridge University Press.
  2. Carle, G. F. & Olson, M. V. ( 1985; ). An electrophoretic karyotype for yeast. Proc Natl Acad Sci U S A 82, 3756–3760.[CrossRef]
    [Google Scholar]
  3. Fell, J. W., Statzell-Tallman, A. & Kurtzman, C. P. ( 2004; ). Lachancea meyersii sp. nov., an ascosporogenous yeast from mangrove regions in the Bahama Islands. Stud Mycol 50, 359–363.
    [Google Scholar]
  4. Felsenstein, J. ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  5. Filipov, G. S. ( 1932; ). Porca fruktovyh konservov, vyzvannaja drozzevym gribkom. Tr Gent Nauchnoissled Bohim Inst Pisc Vkus Prom Narkomsnaba Sojuza SSR 2, 26–32 (in Russian).
    [Google Scholar]
  6. Kodama, K. & Kyono, T. ( 1974; ). Ascosporogenous yeasts isolated from tree exudates in Japan. J Ferment Technol 52, 1–9.
    [Google Scholar]
  7. Kumar, S., Tamura, K. & Nei, M. ( 2004; ). mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef]
    [Google Scholar]
  8. Kurtzman, C. P. ( 1998; ). Zygosaccharomyces Barker. In The Yeasts, a Taxonomic Study, 4th edn, pp. 424–436. Edited by C. P. Kurtzman & J. W. Fell. Amsterdam: Elsevier.
  9. Kurtzman, C. P. ( 2003; ). Phylogenetic circumscription of Saccharomyces, Kluyveromyces and other members of the Saccharomycetaceae, and the proposal of the new genera Lachancea, Nakaseomyces, Naumovia, Vanderwaltozyma and Zygotorulaspora. FEMS Yeast Res 4, 233–245.[CrossRef]
    [Google Scholar]
  10. Kurtzman, C. P. & Robnett, C. J. ( 1998; ). Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences. Antonie van Leeuwenhoek 73, 331–371.[CrossRef]
    [Google Scholar]
  11. Kurtzman, C. P. & Robnett, C. J. ( 2003; ). Phylogenetic relationships among yeasts of the ‘Saccharomyces complex’ determined from multigene sequence analyses. FEMS Yeast Res 3, 417–432.[CrossRef]
    [Google Scholar]
  12. Lachance, M. A. ( 1998; ). Kluyveromyces van der Walt emend. van der Walt. In The Yeasts, a Taxonomic Study, 4th edn, pp. 227–247. Edited by C. P. Kurtzman & J. W. Fell. Amsterdam: Elsevier.
  13. Lee, C. F., Liu, C. H., Young, S. S. & Chang, K. S. ( 2008; ). Kazachstania jiainicus sp. nov., an ascomycetous yeast species isolated from soil in Taiwan. FEMS Yeast Res 8, 114–118.[CrossRef]
    [Google Scholar]
  14. Legakis, Ph. A. ( 1961; ). A contribution to the study of the yeast flora of apples and apple wine. Thesis, University of Athens, Greece.
  15. Naganishi, H. ( 1928; ). The study of five new species of yeast. J Zymol 6, 1–12.
    [Google Scholar]
  16. Naumova, E. S., Serpova, E. V. & Naumov, G. I. ( 2007; ). Molecular systematics of Lachancea yeasts. Biochemistry (Mosc) 72, 1356–1362.[CrossRef]
    [Google Scholar]
  17. Phaff, H. J. & Knapp, E. P. ( 1956; ). The taxonomy of yeasts found in exudates of certain trees and other natural breeding sites of some species of Drosophila. Antonie van Leeuwenhoek 22, 117–130.[CrossRef]
    [Google Scholar]
  18. Suzuki, M. & Nakase, T. ( 2002; ). A phylogenetic study of ubiquinone-7 species of the genus Candida based on 18S ribosomal DNA sequence divergence. J Gen Appl Microbiol 48, 55–65.[CrossRef]
    [Google Scholar]
  19. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  20. Vaughan-Martini, A. & Martini, A. ( 1998; ). Saccharomyces Meyen ex Rees. In The Yeasts, a Taxonomic Study, 4th edn, pp. 358–371. Edited by C. P. Kurtzman & J. W. Fell. Amsterdam: Elsevier.
  21. White, T. J., Bruns, T., Lee, S. & Taylor, J. ( 1990; ). Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In PCR Protocols: a Guide for Methods and Applications, pp. 315–322. Edited by M. A. Innis, D. H. Gelfand, J. J. Sninsky & T. J. White. New York: Academic Press.
  22. Yarrow, D. ( 1998; ). Methods for the isolation, maintenance and identification of yeasts. In The Yeasts, a Taxonomic Study, 4th edn, pp. 77–100. Edited by C. P. Kurtzman & J. W. Fell. Amsterdam: Elsevier.
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