1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 59, Issue 12

gen. nov., sp. nov., a member of the phylum isolated from a forest soil Free

Abstract

A novel golden-yellow-coloured strain, designated RHYL-37, was isolated from a soil sample from a forest of Euphrates poplar () in Xinjiang, China. The cells were strictly aerobic, Gram-staining-negative, heterotrophic, non-spore-forming rods that were motile by gliding. Growth occurred at 17–37 °C (optimum, 30 °C), at pH 5.0–9.0 (optimum, pH 7.0) and with 0–1 % NaCl (w/v; optimum, 0 %). Phylogenetic analysis based on 16S rRNA gene sequences indicated that the novel isolate formed a cluster with members of the genera , , , and in the family ‘’ in the phylum . Sequence similarities with the type strains of recognized species ranged from 90.6 to 94.0 %. The predominant isoprenoid quinone was MK-7 and the major cellular fatty acids were iso-C, iso-C, C and iso-C 3-OH. The DNA GC content was 39.7 mol%. On the basis of the phylogenetic, physiological and chemotaxonomic data, strain RHYL-37 represents a novel genus and species in the family ‘’, for which the name gen. nov., sp. nov. is proposed. The type strain of is RHYL-37 (=CCTCC AB 208240=KCTC 22561).

  • Published Online:
© SGM
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.008763-0
2009-12-01
2024-04-18
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/59/12/3058.html?itemId=/content/journal/ijsem/10.1099/ijs.0.008763-0&mimeType=html&fmt=ahah

References

  1. An, D. S., Lee, H. G., Im, W. T., Liu, O. M. & Lee, S. T.(2007).Segetibacter koreensis gen. nov., sp. nov., a novel member of the phylum Bacteroidetes, isolated from the soil of a ginseng field in South Korea. Int J Syst Evol Microbiol 57, 1828–1833.[CrossRef] [Google Scholar]
  2. Bowman, J. P.(2000). Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov. Int J Syst Evol Microbiol 50, 1861–1868. [Google Scholar]
  3. Bowman, J. P., McCammon, S. A., Brown, M. V., Nichols, D. S. & McMeekin, T. A.(1997). Diversity and association of psychrophilic bacteria in Antarctic sea ice. Appl Environ Microbiol 63, 3068–3078. [Google Scholar]
  4. Buczolits, S., Denner, E. B. M., Vybiral, D., Wieser, M., Kämpfer, P. & Busse, H.-J.(2002). Classification of three airborne bacteria and proposal of Hymenobacter aerophilus sp. nov. Int J Syst Evol Microbiol 52, 445–456. [Google Scholar]
  5. Chun, J., Lee, J.-H., Jung, Y., Kim, M., Kim, S., Kim, B. K. & Lim, Y. W.(2007). EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57, 2259–2261.[CrossRef] [Google Scholar]
  6. Collins, M. D., Pirouz, T., Goodfellow, M. & Minnikin, D. E.(1977). Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100, 221–230.[CrossRef] [Google Scholar]
  7. Doetsch, R. N.(1981). Determinative methods of light microscopy. In Manual of Methods for General Bacteriology, pp. 21–33. Edited by P. Gerhardt, R. G. E. Murray, R. N. Costilow, E. W. Nester, W. A. Wood, N. R. Krieg & G. H. Phillips. Washington, DC: American Society for Microbiology.
  8. Felsenstein, J.(1985). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef] [Google Scholar]
  9. Fitch, W. M.(1971). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20, 406–416.[CrossRef] [Google Scholar]
  10. Garrity, G. M. & Holt, J. G.(2001). The road map to the Manual. In Bergey's Manual of Systematic Bacteriology, 2nd edn, vol. 1, pp. 119–166. Edited by D. R. Boone, R. W. Castenholz & G. M. Garrity. New York: Springer.
  11. Kim, B. Y., Weon, H. Y., Yoo, S. H., Hong, S. B., Kwon, S. W., Stackebrandt, E. & Go, S. J.(2007).Niabella aurantiaca gen. nov., sp. nov., isolated from a greenhouse soil in Korea. Int J Syst Evol Microbiol 57, 538–541.[CrossRef] [Google Scholar]
  12. Mesbah, M., Premachandran, U. & Whitman, W. B.(1989). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef] [Google Scholar]
  13. Rainey, F. A., Ward-Rainey, N., Kroppenstedt, R. M. & Stackebrandt, E.(1996). The genus Nocardiopsis represents a phylogenetically coherent taxon and a distinct actinomycete lineage: proposal of Nocardiopsiaceae fam. nov. Int J Syst Bacteriol 46, 1088–1092.[CrossRef] [Google Scholar]
  14. Reichenbach, H.(1992). The order Cytophagales. In The Prokaryotes, 2nd edn, vol. 4, pp. 3631–3675. Edited by A. Balows, H. G. Trüper, M. Dworkin, W. Harder & K. H. Schleifer. New York: Springer.
  15. Saitou, N. & Nei, M.(1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425. [Google Scholar]
  16. Sangkhobol, V. & Skerman, V. B. D.(1981).Chitinophaga, a new genus of chitinolytic myxobacteria. Int J Syst Bacteriol 31, 285–293.[CrossRef] [Google Scholar]
  17. Smibert, R. M. & Krieg, N. R.(1994). Phenotypic characterization. In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by P. Gerhardt, R. G. E. Murray, W. A. Wood & N. R. Krieg. Washington, DC: American Society for Microbiology.
  18. Tamura, K., Dudley, J., Nei, M. & Kumar, S.(2007).mega 4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24, 1596–1599.[CrossRef] [Google Scholar]
  19. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G.(1997). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef] [Google Scholar]
  20. Weon, H. Y., Kim, B. Y., Yoo, S. H., Lee, S. Y., Kwon, S. W., Go, S. J. & Stackebrandt, E.(2006).Niastella koreensis gen. nov., sp. nov. and Niastella yeongjuensis sp. nov., novel members of the phylum Bacteroidetes, isolated from soil cultivated with Korean ginseng. Int J Syst Evol Microbiol 56, 1777–1782.[CrossRef] [Google Scholar]
  21. Wilson, K.(1987). Preparation of genomic DNA from bacteria. In Current Protocols in Molecular Biology, pp. 2.4.1–2.4.5. Edited by F. M. Ausubel, R. Brent, R. E. Kingston, D. D. Moore, J. G. Seidman, J. A. Smith & K. Struhl. New York: Green Publishing & Wiley-Interscience.
  22. Xie, C. H. & Yokota, A.(2003). Phylogenetic analysis of Lampropedia hyalina based on the 16S rRNA gene sequence. J Gen Appl Microbiol 49, 345–349.[CrossRef] [Google Scholar]
  23. Xie, C. H. & Yokota, A.(2006). Reclassification of [Flavobacterium] ferrugineum as Terrimonas ferruginea gen. nov., comb. nov., and description of Terrimonas lutea sp. nov., isolated from soil. Int J Syst Evol Microbiol 56, 1117–1121.[CrossRef] [Google Scholar]
  24. Yoon, M. H. & Im, W. T.(2007).Flavisolibacter ginsengiterrae gen. nov., sp. nov. and Flavisolibacter ginsengisoli sp. nov., isolated from ginseng cultivating soil. Int J Syst Evol Microbiol 57, 1834–1839.[CrossRef] [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.008763-0
Loading
Parasegetibacter luojiensis gen. nov., sp. nov., a member of the phylum Bacteroidetes isolated from a forest soil
Int J Syst Evol Microbiol 59, 3058 (2009); https://doi.org/10.1099/ijs.0.008763-0
/content/journal/ijsem/10.1099/ijs.0.008763-0
/content/journal/ijsem/10.1099/ijs.0.008763-0
Loading

Data & Media loading...

Supplements

vol. , part 12, pp. 3058 - 3062

Fatty acid profiles (%) of strain RHYL-37 and type strains of related taxa. [PDF](80 KB)

PDF

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error